Differences and Clinical Significance of Serum 25-Hydroxyvitamin D3 and Vasohibin-1 (VASH-1) Levels in Patients with Diabetic Nephropathy and Different Renal Injuries

Liu, Hui; Wang, Dongyan; Tang, Jingnan; Yu, Lan; Su, Shanshan

doi:10.2147/dmso.s405554

Cited by 1 publication

(1 citation statement)

References 44 publications

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“…VASH-1, an endogenous negative feedback regulator of angiogenesis, plays a role in regulating various factors to inhibit angiogenesis. It has been reported in diseases such as atherosclerosis, diabetic nephropathy, and pulmonary fibrosis [18,33,34]. Notably, studies have suggested that, apart from its association with the progressive decline in renal function [4], VASH-1 might potentially exert protective effects through negative feedback in renal diseases [18,35].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-10 Family Promotes Renal Fibrosis through the VASH-1/Smad3 Pathway

Shuai,

Xu,

Zhao

et al. 2024

IJMS

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Renal fibrosis (RF) stands as a pivotal pathological process in the advanced stages of chronic kidney disease (CKD), and impeding its progression is paramount for delaying the advancement of CKD. The miR-10 family, inclusive of miR-10a and miR-10b, has been implicated in the development of various fibrotic diseases. Nevertheless, the precise role of miR-10 in the development of RF remains enigmatic. In this study, we utilized both an in vivo model involving unilateral ureteral obstruction (UUO) in mice and an in vitro model employing TGF-β1 stimulation in HK-2 cells to unravel the mechanism underlying the involvement of miR-10a/b in RF. The findings revealed heightened expression of miR-10a and miR-10b in the kidneys of UUO mice, accompanied by a substantial increase in p-Smad3 and renal fibrosis-related proteins. Conversely, the deletion of these two genes led to a notable reduction in p-Smad3 levels and the alleviation of RF in mouse kidneys. In the in vitro model of TGF-β1-stimulated HK-2 cells, the co-overexpression of miR-10a and miR-10b fostered the phosphorylation of Smad3 and RF, while the inhibition of miR-10a and miR-10b resulted in a decrease in p-Smad3 levels and RF. Further research revealed that miR-10a and miR-10b, through binding to the 3’UTR region of Vasohibin-1 (VASH-1), suppressed the expression of VASH-1, thereby promoting the elevation of p-Smad3 and exacerbating the progression of RF. The miR-10 family may play a pivotal role in RF.

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