Difference spectrophotometric method for the determination of Fluphenazine hydrochloride in tablets using peroxomonosulfate

Abstract

The oxidative derivatization method using potassium hydrogenperoxomonosulfate for the indirect spectrophotometric determination of Fluphenazine hydrochloride is presented. Potassium hydrogenperoxomonosulfate is introduced as a derivatizing agent for Fluphenazine hydrochloride, yielding the sulfoxide. This reaction product was successfully used for the spectrophotometric determination of the Fluphenazine hydrochloride. The UV spectroscopic detection of the sulfoxide proved to be a more robust and sensitive method. The elaborated method allowed the determination of Fluphenazine hydrochloride in the concentration range of 0.2-30 µg mL-1. The molar absorptivity at 349 nm is 5.6×103 (dm3cm-1mol-1). The limit of quantification, LOQ (10S) is 0.24 µg/mL. A new spectrophotometric technique was developed and the possibility of quantitative determination of Fluphenazine hydrochloride in tablets 5.0 mg was demonstrated. The present method is precise, accurate and excipients did not interfere. RSD for Fluphenazine Hydrochloride 5.0 mg tablets was 1.37 %.

Spectrophotometric Assay of Prothipendyl through the Determination of Its Sulfoxide

“…Due to their valuable therapeutic and pharmacological properties, phenothiazine and azaphenothiazine derivatives are the subject of many scientific studies. A review of the literature shows that methods for the determination of phenothiazine and azaphenothiazine derivatives mainly include chromatographic (6)(7)(8)(9) and spectrophotometric (10)(11)(12).…”

“…However, the real great importance of difference spectrophotometry is in quantitative analysis as a consequence of its potential to increase the selectivity of the measurement(16).The aim of this research is to develop a new and simple method for prothipendyl hydrochloride determination. It seems promising to carry out the analysis of prothipendyl hydrochloride in the form of the corresponding stable S-oxide, which is easily obtained in a slightly acidic medium using aliphatic diperoxyacids or inorganic Caro peroxoacid(10,15,17,18).in water and a water-ethanol solution 1:1 (v/v) respectively. All other used chemicals were analytical grade.…”