Difference in Onset of Warm Ischemia and Reperfusion Injury between Parenchymal and Endothelial Cells of the Liver. Evaluation by Purine Nucleoside Phosphorylase and Hyaluronic Acid

Nakagami, Mikio; Morimoto, Tetsuya; Mitsuyoshi, Akira; Mashima, Susumu; Shimabukuro, Takashi; Yamaoka, Yoshio

doi:10.1006/jsre.1996.0183

Cited by 16 publications

(10 citation statements)

References 5 publications

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“…This result was surprising, because plasma transaminases activity rapidly returns to basal values in acute liver injury. 14 In addition, Nakagami et al 15 have shown much less (3 to 14 times) in liver enzyme release in plasma after 60 minutes ischemia and 24 hours reperfusion in the dog than after 1 hour of reperfusion.…”

Section: Resultsmentioning

confidence: 99%

Hepatoprotective Effect of Endogenous Nitric Oxide During Ischemia–Reperfusion in the Rat

Cottart¹,

Do²,

Blanc

et al. 1999

Hepatology

114

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Section: Resultsmentioning

confidence: 99%

Hepatoprotective Effect of Endogenous Nitric Oxide During Ischemia–Reperfusion in the Rat

Cottart¹,

Do²,

Blanc

et al. 1999

Hepatology

114

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“…This fact could be related to the difference in the onset of warm ischemia and reperfusion injury. Nakagami et al [28] have recently suggested that a time lag occurs in the onset of injury between parenchymal and endothelial cells, and that endothelial cells are temporarily earlier in failing than parenchymal cells when the liver is exposed to short-term warm ischemia and subsequent reperfusion. The timing of our determinations are probably too short to obtain any difference with respect to hepatocellular damage (although it appears that it does not get to its greatest level).…”

Section: Discussionmentioning

confidence: 99%

Liver conditioning after cardiac arrest: the use of normothermic recirculation in an experimental animal model

García-Vaidecasas¹,

Tabet²,

Valero

et al. 1998

Transplant Int

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Preliminary results of this study were presented at the XVI World Congress of the Transplantation Society, Barcelona, Spain, August 1996 J. C. Garcia Abstract The aim of this study was to compare the possible role of normothermic recirculation with the role of liver transplants from nonheart-beating donor pigs after 20 min of cardiac arrest. Three groups were studied, of which two were control groups: group 1, in which the liver was harvested from a heart-beating donor; group 2, in which the liver was harvested after a period of cardiac arrest followed by total body cooling; and group 3, in which the liver was procured as in group 2, but including a period of 30 rnin of cardiopulmonary bypass and tissue oxygenation at 37 "C before total body cooling. Survival at 5 days; endothelial (hyaluronic acid) and hepatocellular damage (AST, ALT, and a-GST); adenine nucleotides (energy charge), and histological changes were evaluated. Normothermic recirculation during 30 rnin showed a significant effect on survival ( p = .03), endothelial damage (p < .05), and histological changes after reperfusion (p = .04). Cardiopulmonary bypass significantly increased the energy charge during the normothermic recirculation period ( p = .001). Moreover, this study shows that a significant survival (100 % ) can be achieved with a liver allograft after 20 rnin of cardiac arrest. Although the liver suffers a major insult in terms of endothelial damage and hepatocellular damage, lesions caused by the ischemic injury are reversible. Histological changes also indicate lesion reversibility, since they almost disappear after 5 days.

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“…Nakagami et al [29] have suggested that a time lag occurs in the onset of injury between parenchymal and endothelial cells and that endothelial cells are temporarily earlier in failing than parenchymal cells when the liver is exposed to shortterm warm ischemia and subsequent reperfusion. Although both substances showed an overall significant relationship with the period of CA (MANOVA), HA achieved statistically significant differences at the time of NR, while GST did so at the time of RP.…”

Section: Evaluation Of the Ischemic Injurymentioning

confidence: 99%

Evaluation of Ischemic Injury during Liver Procurement from Non-Heart-Beating Donors

García‐Valdecasas¹,

Tabet²,

Valero³

et al. 1999

Eur Surg Res

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The aim of this study was to assess liver viability after different periods of cardiac arrest and the predictive value of two markers of ischemia-reperfusion injury. Methods: A pig liver transplantation model of non-heart-beating donors was studied. Four donor groups were designed; three groups were submitted to different periods of cardiac arrest (20, 30 and 40 min), and the fourth group served as the control group (without cardiac arrest). In the non-heart-beating donor groups, normothermic recirculation was established 30 min prior to total body cooling. Aminotransferase, α-glutathione-S-transferase, and hyaluronic acid determinations as well as liver biopsies, were serially performed. Results: Although hepatocellular function could be preserved after 40 min of cardiac arrest, histological lesions at 5 days were considered irreversible due to the presence of a necrotic biliary tract. An overall significant relationship was found between the time period of cardiac arrest (20, 30 or 40 min) and the levels of hyaluronic acid (p = 0.004) or α-glutathione-S-transferase (p = 0.01) obtained during liver procurement and transplantation. Conclusions: The period of cardiac arrest is the determinant factor of liver viability after liver transplantation from non-heart-beating donors. As early markers of endothelial or hepatocellular damage, hyaluronic acid or α-glutathione-S-transferase levels may help to evaluate the ischemic injury of a potential donor.

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Difference in Onset of Warm Ischemia and Reperfusion Injury between Parenchymal and Endothelial Cells of the Liver. Evaluation by Purine Nucleoside Phosphorylase and Hyaluronic Acid

Cited by 16 publications

References 5 publications

Hepatoprotective Effect of Endogenous Nitric Oxide During Ischemia–Reperfusion in the Rat

Hepatoprotective Effect of Endogenous Nitric Oxide During Ischemia–Reperfusion in the Rat

Liver conditioning after cardiac arrest: the use of normothermic recirculation in an experimental animal model

Evaluation of Ischemic Injury during Liver Procurement from Non-Heart-Beating Donors

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