Difference in measured and indicated stirring speed of a commercial aggregometer

Stibbe, J.; Grashuis, J. L.; Gomes, Milena da Rocha

doi:10.1016/0049-3848(79)90262-7

Cited by 1 publication

(2 citation statements)

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“…One of the effects of the gelatin is an increase in viscosity. As we have shown before, differences in viscosity (buffer, Haemaccel 35 mg/ml or PPP) did not affect the stirring speed of the stirring rod in the aggregometer cuvette [12], Although the stirring speed is not influenced by an increased viscosity, the effective mixing of the aggregation inducer and products secreted by the platelet may be decreased, thus resulting in a decreased aggregation response. However, as one of the gelatins, Haemaccel, has an inhibitory, while the others, Plasmagel and Plasmion, have an enhancing effect on aggregation when compared with the control, this explanation is not very likely.…”

Section: Discussionmentioning

confidence: 84%

See 1 more Smart Citation

Effects of the Gelatin Plasma Substitutes Haemaccel®, Plasmagel® and Plasmion® (Geloplasma®) on Collagen-, ADP- and Adrenaline-Induced Aggregation of Human Platelets in vitro

Stibbe¹,

Plas²,

Ong³

et al. 1981

Pathophysiol Haemos Thromb

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The effect of some gelatin plasma substitutes (Haemaccel^®, Plasmagel^® and Plasmion^® (Geloplasma^®), which are widely used in Europe) on collagen-, ADP- and adrenaline-induced platelet aggregation in human PRP in vitro was studied under controlled conditions (pH, electrolyte composition). Haemaccel inhibited these aggregations, both in citrated as well as in heparinised PRP, whereas they were enhanced by both Plasmagel and Plasmion as compared to the appropriate control. Increasing the concentration of the inducer overcame the inhibition by Haemaccel. Haemaccel inhibited, while Plasmion enhanced ¹⁴C-serotonin release induced by collagen, ADP or adrenaline. Also in the presence of indomethacin (90 µM) Haemaccel inhibited aggregation induced by high concentrations of collagen and the primary aggregation induced by ADP and adrenaline, while Plasmion enhanced these aggregations. The inhibition by Haemaccel was not caused by binding of Ca²⁺ to Haemaccel.

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Section: Discussionmentioning

confidence: 84%

“…Ont., Canada) using a stirring speed of 450 rpm (the intentional stirring speed was 950 rpm. During this study we measured the actual stirring speed and found it to be 450 rpm while the rpm-meter on the aggregometer indicated 950 rpm [12]). Final platelet counts were 200-300 X 109 cells/1.…”

Section: Aterials and Methodsmentioning

confidence: 99%