Difference in EGFR expression and mean vascular density in normal oral mucosa, oral epithelial dysplasia and oral squamous cell carcinoma.

Fernández, Alejandra; Fernández, Javier D.; Marshall, Maureen; Martínez, Rolando; Niklander, Sven; Haidar, Ziyad S.

doi:10.17126/joralres.2017.016

Cited by 4 publications

(4 citation statements)

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“…Fernandez et al (2017) reported the presence of EGFR in normal oral mucosa, indicative of the proliferation of epithelial cells. Receptors were found to be not only limited to basal stratum cells but were also expressed by suprabasal cells [38]. Similar fi ndings regarding the distribution of EGFR immunoreactive epithelial cells were observed in soft tissues of the control group and in CLP aff ected tissues in our patients.…”

Section: Discussionsupporting

confidence: 88%

Prevalence of Egfr, Ki-67, Nf-Κb, Ma-1 marker in cleft affected tissue of soft palate

Tellermann

Reiser

Pilmane

2019

PoA

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Introduction. Midline orofacial defects bear a high degree of morbidity for the affected individual, often associated with retardation in neonatal development and the need for surgical intervention. The etiology of the deformities, despite their high prevalence, remains, however, largely unknown. We report on the evaluation and quantification of proliferative and inflammatory markers in the cleft affected soft palate. Material and methods. This study included soft palate samples of eight cleft lip and palate affected individuals and a control group of six soft tissue specimens obtained during correctional surgery of hyperdentia. All samples were processed via immunohistochemistry for marker EGFR, NF-κB, Ki-67, Ma-1. The results were evaluated semi-quantitatively and statistically analysed by IBM SPSS 25.0. Results. Histopathological signs of inflammatory changes were continuous in cleft affected specimen. The quantitative distribution of the markers in the cleft affected group displayed a significant correlation between EGFR, Ki-67 and NF-κB (p< 0.05), along with a correlation among Ki-67 and NF-κB (p< 0.05). Immunoreactive structures in control group showed lower numbers in all evaluated specimen. A statistical significance between cleft affected and non-cleft tissue was observed in EGFR and Ma-1 (p< 0.05). Conclusion. Results are suggestive of a tissue phenotype modification in cleft affected palate. Observation of distribution and statistical correlation hint towards the involvement of epithelial growth inducer EGFR and inflammatory/ proliferative marker in epithelial changes.

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Section: Discussionsupporting

confidence: 88%

Prevalence of Egfr, Ki-67, Nf-Κb, Ma-1 marker in cleft affected tissue of soft palate

Tellermann

Reiser

Pilmane

2019

PoA

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“…With a global incidence of 500,000 per annum, squamous cell carcinoma has been reported to be the most prevalent cancer of the oral cavity [ 1 ]. In South Asia, head and neck cancers are the third leading cause of cancer-related morbidity and mortality [ 2 – 4 ].…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical expression of epidermal growth factor receptor (EGFR) in South Asian head and neck squamous cell carcinoma: association with various risk factors and clinico-pathologic and prognostic parameters

et al. 2018

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BackgroundIn this study, we intend to determine the immunohistochemical expression of EGFR in cases of head and neck squamous cell carcinoma and its association with prognostic clinico-pathologic features.MethodsA total of 115 cases of head and neck squamous cell carcinoma treated at Liaquat National Hospital, Karachi, Pakistan, were included in the study. Clinico-pathologic features, risk factors, and recurrence status of cases were evaluated, and EGFR immunohistochemistry was performed.ResultsIn our study, 52 cases (45.2%) of head and neck SCC were positive and 10 cases (8.7%) were focal positive for EGFR expression, while 53 cases (46.1%) were negative for EGFR expression. High EGFR expression (> 70%) was noted in 6.1% (7 cases), while 12.2% (14 cases) and 26.1% (30 cases) revealed 51–70% and 11–50% EGFR expression respectively. On the basis of intensity, strong EGFR expression was noted in 13.9% (16 cases) while 16.5% (19 cases) and 23.5% (27 cases) revealed intermediate and weak EGFR expression respectively. Significant association of EGFR expression was noted with tumor stage and disease-free survival.ConclusionWe found a significant association of EGFR expression with tumor stage and disease-free survivals, which are the most important prognostic factors in head and neck squamous cell carcinoma; therefore, EGFR expression can help as a prognostic biomarker in head and neck squamous cell carcinoma. On the other hand, we suggest that molecular studies should be performed in squamous cell carcinoma of head and neck in our setup to identify patients that can avail response from anti-EGFR therapy.

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“…Studies on CD31 expression revealed no significant difference in the expression of CD31 between fibromas, OEDs, and OSCCs, as well as in normal mucosa, adenoma and adenocarcinoma of colon. It had been proposed that these conflicting results could be attributed to the use of different markers and methods in the micro vessel counting, antigenic retrieval, and inter-observer variations; that all of these factors affect the assessment of MVD 19 . Moreover, the inability of CD31 to distinguish newly formed tumoral blood vessels from normal pre-existing ones in both neoplastic and non-neoplastic tissues 20 .…”

Section: Discussionmentioning

confidence: 99%

Stromal Cell Diversity in Tumor Microenvironment Promote Invasion of Oral Squamous Cell Carcinoma

wahba,

Mourad

2023

Egyptian Dental Journal

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Background and objectives: Epithelial neoplastic cells only prosper in aberrant microenvironmental conditions. That aberrant environment contains an altered extracellular matrix and multiple stromal cells including, fibroblasts, macrophages, and endothelial cells. In parallel with neoplastic cells, the previous cell types have a pivotal role in tumor pathogenesis and progression. The current study evaluated changes in stromal cells in the tumor microenvironment in patients diagnosed with oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).Method: Thirty-six archival OED and 83 archival OSCC cases were included in our research. Immunohistochemistry was performed for α-SMA, CD31, and CD163. Macrophage and Fibroblast counts were performed manually. We also morphologically determined the microvessel density (MVD). Results:In different types of epithelial dysplasia (mild, moderate, and severe), the number of stromal cells that express α-SMA, CD31, and CD163 increased from mild to severe epithelial dysplasia. As well, in different variants of oral squamous cell carcinoma (well, moderate, and poorly), there was a significant difference in stromal cells that express α-SMA, CD31 and CD163. Conclusion:These findings reveal that changes in stromal cells beneath oral epithelial dysplasia and in OSCC stroma might play a potential regulatory role in OSCC invasion as well as progression.

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Difference in EGFR expression and mean vascular density in normal oral mucosa, oral epithelial dysplasia and oral squamous cell carcinoma.

Cited by 4 publications

References 15 publications

Prevalence of Egfr, Ki-67, Nf-Κb, Ma-1 marker in cleft affected tissue of soft palate

Prevalence of Egfr, Ki-67, Nf-Κb, Ma-1 marker in cleft affected tissue of soft palate

Immunohistochemical expression of epidermal growth factor receptor (EGFR) in South Asian head and neck squamous cell carcinoma: association with various risk factors and clinico-pathologic and prognostic parameters

Stromal Cell Diversity in Tumor Microenvironment Promote Invasion of Oral Squamous Cell Carcinoma

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