Difference in cell surface 5'-nucleotidase activity, protein iodination and membrane properties between mouse lymphoma and rat adenocarcinoma cell variants with low and high-metastatic potential

Haeffner, E.W.; Doenges, Karl H.; Buchholz, J.

doi:10.1016/0020-711x(88)90177-2

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Pro and eukaryotic cells bear a net negative electrical charge which influences the adhesiveness and selective affinity between cells, playing an important role in morphogenesis during the embryonic development (Schaeffer et al 1973), in the malignancy and ability of the cells to metastasize (Haeffner et al 1988, Carter et al 1989) and in parasite-host cell interaction (Meirelles et al 1984, Araújo-Jorge et al 1992, Monteiro et al 1998.…”

Section: Surface Chargementioning

confidence: 99%

The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.

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Section: Surface Chargementioning

confidence: 99%

The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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Differential Alterations in the Distribution of Three Phosphatase Enzymes During the Plasma Membrane Transformation of Uterine Epithelial Cells in the Rat

Bucci

Murphy

1999

Cell Biology International

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Ultrastructural and light microscopic cytochemical methods were used to study the distribution and changes in distribution of three phosphatase enzymes: 5'-nucleotidase (5N); thiamine pyrophosphatase (TPP); and adenosine triphosphatase (ATP) in the rat endometrium during early pregnancy up to the time of blastocyst attachment. The authors were particularly interested in changes in the apical plasma membrane and reaction product for all three enzymes was clearly localized along this membrane especially on day 1 of pregnancy. However, the three enzymes showed markedly different patterns of organization of reaction product at later times during early pregnancy. 5N, while showing a continuous lining along the microvilli on day 1 was virtually undetectable by day 6. TPP was also strongly present apically on day 1, but reaction product was not always found as a continuous lining. Again, by day 6, there was no presence of this enzyme along the apical surface. ATP differed from the other two in that it produced a strong, and relatively unchanged reaction product along the apical plasma membrane from day 1 through to day 6 of pregnancy. The changes in distribution of these enzymes was particularly obvious at the electron microscopic level and we consider their contribution to the process of 'plasma membrane transformation' of early pregnancy.

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Hormonal Control of Enzyme Activity During the Plasma Membrane Transformation of Uterine Epithelial Cells

Bucci

Murphy

2001

Cell Biology International

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Ultrastructural and light microscopic catalytic histochemical methods were used to study the distribution and changes in distribution of four phosphatase enzymes; alkaline phosphatase, 5'-nucleotidase, thiamine pyrophosphatase and adenosine triphosphatase in uterine epithelial cells in response to the ovarian hormones, oestrogen, progesterone or a combination of both used in different regimes on ovariectomised rats. Reaction product for all four enzymes was clearly localised in the epithelial cells, especially with oestrogen priming. However, the four enzymes showed markedly different patterns of organisation of reaction product in response to other hormonal treatments. Our findings clearly show that the expression of these enzymes is under ovarian hormonal control. However, while all of the enzymes are upregulated by oestrogen, the response to progesterone is variable, which can upregulate or downregulate different enzymes. The findings are particularly obvious at the electron microscopic level on the apical plasma membrane of the uterine epithelial cells, which was the main focus of our study.

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Difference in cell surface 5'-nucleotidase activity, protein iodination and membrane properties between mouse lymphoma and rat adenocarcinoma cell variants with low and high-metastatic potential

Cited by 4 publications

References 32 publications

The surface charge of trypanosomatids

The surface charge of trypanosomatids

Differential Alterations in the Distribution of Three Phosphatase Enzymes During the Plasma Membrane Transformation of Uterine Epithelial Cells in the Rat

Hormonal Control of Enzyme Activity During the Plasma Membrane Transformation of Uterine Epithelial Cells

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