Difference between the cell wall roughnesses of mothers and daughters of Saccharomyces cerevisiae subjected to high pressure stress

Carvalho, Lauanda Milanez; Spagnol, Brígida A.A.; Carneiro, Tarcio; Costa, Ane Catarine Tosi; Quadros, Oeber de Freitas; Ventura, José Aires; Biasi, R.S. de; Fernandes, Antônio Alberto Ribeiro; Fernandes, Patrícia Machado Bueno

doi:10.1016/j.micron.2021.103091

Cited by 2 publications

(1 citation statement)

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“…R a , R q , and R max are common parameters for characterizing roughness (Moura et al 2021 ). Therein, R a is the absolute value of the height deviation measured relative to the center plane in the examined area; R q is the root mean square parameter corresponding to R a ; R max is the height difference between the highest and lowest points relative to the center line within the contour length in the cross-sectional contour profile graphs.…”

Section: Discussionmentioning

confidence: 99%

Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene

Yao,

Ma,

Ran

et al. 2024

Appl Microbiol Biotechnol

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Laccase, a copper-containing polyphenol oxidase, is an important green biocatalyst. In this study, Laccase Lcc5 was homologous recombinantly expressed in Coprinopsis cinerea and a novel strategy of silencing chitinase gene expression was used to enhance recombinant Lcc5 extracellular yield. Two critical chitinase genes, ChiEn1 and ChiE2, were selected by analyzing the transcriptome data of C. cinerea FA2222, and their silent expression was performed by RNA interference (RNAi). It was found that silencing either ChiEn1 or ChiE2 reduced sporulation and growth rate, and increased cell wall sensitivity, but had no significant effect on mycelial branching. Among them, the extracellular laccase activity of the ChiE2-silenced engineered strain Cclcc5-antiChiE2-5 and the control Cclcc5-13 reached the highest values (38.2 and 25.5 U/mL, respectively) at 250 and 150 rpm agitation speeds, corresponding to productivity of 0.35 and 0.19 U/mL·h, respectively, in a 3-L fermenter culture. Moreover, since Cclcc5-antiChiE2-5 could withstand greater shear forces, its extracellular laccase activity was 2.6-fold higher than that of Cclcc5-13 when the agitation speed was all at 250 rpm. To our knowledge, this is the first report of enhanced recombinant laccase production in C. cinerea by silencing the chitinase gene. This study will pave the way for laccase industrial production and accelerate the development of a C. cinerea high-expression system. Key points • ChiEn1 and ChiE2 are critical chitinase genes in C. cinerea FA2222 genome. • Chitinase gene silencing enhanced the tolerance of C. cinerea to shear forces. • High homologous production of Lcc5 is achieved by fermentation in a 3-L fermenter.

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Section: Discussionmentioning

confidence: 99%