Dietzia papillomatosis sp. nov., a novel actinomycete isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis

Abstract

An actinomycete isolated from an immunocompetent patient suffering from confluent and reticulated papillomatosis was characterized using a polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties that were consistent with its assignment to the genus Dietzia and it formed a distinct phyletic line within the Dietzia 16S rRNA gene tree. It shared a 16S rRNA gene sequence similarity of 98.3 % with its nearest neighbour, the type strain of Dietzia cinnamea, and could be distinguished from the type strains of all Dietzia species using a combination of phenotypic properties. It is apparent from genotypic and phenotypic data that the organism represents a novel species in the genus Dietzia. (Mayilraj, et al., 2006), D. natronolimnaea from a moderately saline and alkaline East African soda lake (Duckworth et al., 1998) and D. psychralcaliphila was isolated from a drain pool of a fish-egg-processing plant (Yumoto et al., 2002). Species of the genus Dietzia have been reported as potential human pathogens in an immunocompetent patient (Pidoux et al., 2001) and in immunocompromised patients (BemerMelchior et al., 1999;Yassin et al., 2006).The aim of the present investigation was to determine the taxonomic position of an actinomycete that had been isolated from the skin of an immunocompetent patient with confluent and reticulated papillomatosis and presumptively assigned to the genus Dietzia (Natarajan et al., 2005). The isolate was the subject of a polyphasic taxonomic investigation which showed that it warrants recognition as a novel species of the genus Dietzia.Strain N 1280 T was isolated from skin scrapings from a patient suffering from confluent and reticulated papillomatosis, as described by Natarajan et al. (2005). The organism was maintained on glucose-yeast extract agar (GYEA; Gordon & Mihm, 1962) at room temperature and as glycerol suspensions (20 %, v/v) at 220 u C. Biomass required for chemotaxonomic and 16S rRNA gene sequence analyses was obtained by growing the novel strain in shake flasks of glucose-yeast extract (GYE) brothThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain N 1280T is AY643401.

Dietzia papillomatosis Bacteremia

“…Our observations of altered life cycle morphology of D. papillomatosis is in accordance with a previous report that describes D. papillomatosis as having forms of both rods and cocci (1). We propose a novel habitat for D. papillomatosis in blood.…”

“…We propose a novel habitat for D. papillomatosis in blood. In comparison to the current literature, the only previously known presence of this bacterium in humans is from skin scrapings in patients with confluent and reticulated papillomatosis (CARP) (1). Besides a reaction to bacterial infection, CARP has also been linked to several other causes, including a reaction to Malassezia, a reaction to UV light, and an eruption related to endocrinopathy.…”

First Isolation of Dietzia cinnamea from a Dog Bite Wound in an Adult Patient

“…can be reliably differentiated from members of other mycolic acid-containing genera by using 16S rRNA gene sequencing analysis (8,11,13). Although the potential of D. cinnamea to cause an infection was not able to be shown in our study, as an effective antimicrobial treatment was introduced without delay, previous reports by other authors suggest that Dietzia species have the potential to act as opportunistic pathogens (1,7,12,13,19). The cell structure of Dietzia spp.…”

Identification of Atypical Rhodococcus -Like Clinical Isolates as Dietzia spp. by 16S rRNA Gene Sequencing

“…The genus Dietzia has been established very recently and includes environmental isolates with great resemblance in morphology and colony appearance to R. equi (13). Several authors have reported the isolation of Dietzia strains, notably Dietzia maris (3, 18) but also Dietzia papillomatosis (12) and Dietzia cinnamea (25), from human clinical material. The 16S rRNA genes of isolates CA138, CA150, CA160, and CA167 showed Ͼ99% identity to the corresponding gene from D. maris strain DSM 43672 (Table 1), the type strain of the genus Dietzia (15).…”