Diethylaminoethyl chitosan–hyaluronic acid polyelectrolyte complexes

Raik, Sergei V.; Gasilova, Ekaterina R.; Dubashynskaya, Natallia V.; Добродумов, А. В.; Skorik, Yury A.

doi:10.1016/j.ijbiomac.2019.10.054

Cited by 26 publications

(20 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hyaluronic acid-chitosan NPs synthesised in this research via ionic gelation have a smooth surface, are spherical, and their diameters are around 200–300 nm. Similar findings, as well as for particle size and particle yield, are reported by a number of authors, namely, Raik [ 28 ], Pornpitchanarong [ 33 ], Zhou [ 26 ] and de la Fuente [ 27 ]. Relevantly, a higher TPP concentration leads to a smaller particle size as a result of a more compacted nanostructure derived from strong electrostatic interactions [ 27 , 31 ].…”

Section: Discussionsupporting

confidence: 88%

“…For this research, chitosan (CS), a chitin-derived natural polysaccharide, and hyaluronic acid (HA), a non-sulphated glycosaminoglycan, were selected to create the nanoparticles. While hyaluronan is used in a variety of clinical applications [ 26 ] due to its non-immunogenic, mucoadhesive, analgesic and biodegradable properties [ 27 , 28 ], chitosan is biocompatible and biodegradable, and has high stability and low immunogenicity [ 26 , 29 ]. These have been used to fabricate tissue engineering scaffolds, which promote cell attachment, proliferation and viability [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…While hyaluronan is used in a variety of clinical applications [ 26 ] due to its non-immunogenic, mucoadhesive, analgesic and biodegradable properties [ 27 , 28 ], chitosan is biocompatible and biodegradable, and has high stability and low immunogenicity [ 26 , 29 ]. These have been used to fabricate tissue engineering scaffolds, which promote cell attachment, proliferation and viability [ 28 ]. Relevantly, chitosan can create nanostructures through electrostatic interactions, hydrophobic interactions, hydrogen bonds and van der Waals forces [ 29 ], and hyaluronic acid has the ability to target specific cells by binding with receptors on the cell surface such as CD44 [ 30 , 31 ], which makes it suitable for drug delivery targeted at tumours [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…HA and CS nanoparticles have been synthesised by a number of methods, including complex coacervation [ 26 ] and ionic gelation [ 27 , 32 , 33 ]. Briefly, HA, a relatively high-molecular weight weak polyanion, and CS, a lower molecular weight weak polycation, create an asymmetric polyelectrolyte pair [ 28 , 34 ]. They bind together due to electrostatic interactions between the free amino groups in chitosan and the carboxyl group in hyaluronan [ 28 ] ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Controlled-sized nanostructures, such as HA-CS polyelectrolyte complex nanoparticles, are particularly useful for TE applications [ 29 ]. These nanosystems interact well with cell surfaces, which are negatively charged [ 28 ], and therefore offer prolonged residence time at the target site [ 28 , 35 ]. As reported by de la Fuente [ 27 ], systems incorporating HA have been used to modify surfaces with the aim of improving their adhesive properties, and have excellent capacity to associate either hydrophilic or hydrophobic macromolecules [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Electrospun Fibres with Hyaluronic Acid-Chitosan Nanoparticles Produced by a Portable Device

Fuenteslópez

2020

Nanomaterials

View full text Add to dashboard Cite

Electrospinning is a versatile technique to produce nano/microscale fibrous scaffolds for tissue engineering and drug delivery applications. This research aims to demonstrate that hyaluronic acid-chitosan (HA-CS) nanoparticles can be electrospun together with polycaprolactone (PCL) and gelatine (Ge) fibres using a portable device to create scaffolds for tissue repair. A range of polymer solutions of PCL-gelatine at different weight/volume concentrations and ratios were electrospun and characterised. Fibre–cell interaction (F11 cells) was evaluated based on cell viability and proliferation and, from here, a few polymer blends were electrospun into random or aligned fibre arrangements. HA-CS nanoparticles were synthesised, characterised, and used to functionalise electrospun fibres (8% w/v at 70 PCL:30 Ge), which were chosen based on cell viability. Different concentrations of HA-CS nanoparticles were tested to determine cytotoxicity. A single dosage (1 × 10−2 mg/mL) was associated with higher cell proliferation compared with the cell-only control. This nanoparticle concentration was embedded into the electrospun fibres as either surface modification or blend. Fibres with blended NPs delivered a higher cell viability than unmodified fibres, while NP-coated fibres resulted in a higher cell proliferation (72 h) than the NP-blended ones. These biocompatible scaffolds allow cell attachment, maintain fibre arrangement, promote directional growth and yield higher cell viability.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Electrospun Fibres with Hyaluronic Acid-Chitosan Nanoparticles Produced by a Portable Device

Fuenteslópez

2020

Nanomaterials

View full text Add to dashboard Cite

show abstract

Colloidal Polyelectrolyte Complexes from Hyaluronic Acid: Preparation and Biomedical Applications

Cerf

2022

Small

View full text Add to dashboard Cite

nanocomplexes, [8,9] nanocomposites, [10,11] nanoassemblies [12] and coacervates. [13,14] Such colloidal systems, especially when prepared from biopolymers, have been extensively investigated due to their promising properties for numerous applications in biomedical fields. [1] Their merits come from the combination of three aspects: nanomedicine, biomaterials, and green chemistry. With the emergence of nanomedicine, the use of nanocarriers can offer tremendous advantages in drug encapsulation and delivery, namely enhancing the chemical stability of drugs and improving drug solubility and bioavailability. [15] Due to their small size and the possibility of surface modification with biological ligands or molecular imprinting, nanocarriers can easily cross biological barriers, which have limited pore sizes (fenestrations) under 1 micron in most cases, then target and enter the tissues or cells of interest. [16,17] Due to these aspects and the possibility of controlling drug distribution and release through particle engineering, nanocarriers can thus reduce systemic side effects and enhance the therapeutic efficacy of drugs. [15] Nanosystems can also offer unique advantages for biomedical imaging with their ability of sensing, image enhancement, and incorporating concomitantly therapeutic agents for theranostics, that is, simultaneous therapeutic and diagnostic applications. [18][19][20] When constructed from biomaterials, especially naturally occurring polysaccharides or proteins, PECs can become significantly biocompatible and biodegradable with much less immunogenicity and toxicity. [21] Besides such biorelevant aspects, the elaboration of biopolymerbased PECs is also in accordance with green chemistry principles since their preparation process usually requires only gentle mixing of polyelectrolyte solutions at room temperature, which is a simple and rapid procedure without the need for chemical agents, surfactants, organic solvents or high mechanical or thermal energy. [15] Beyond the above-mentioned basic advantages, the potential of colloidal PEC systems is increased by the inherent biological and chemical properties of individual constituent polymers. Hyaluronic acid (HA), also called hyaluronan to generally mention both its acid and salt forms, has been one of the most common polyanions among several biopolymers reported so far for the elaboration of colloidal PECs. The significant attention dedicated to HA in biomedical fields is associated with its unique advantages, stemming from not only its outstanding biocompatibility but also interesting biological activities. [22] Hyaluronic acid (HA) is a naturally occurring polysaccharide which has been extensively exploited in biomedical fields owing to its outstanding biocompatibility. Self-assembly of HA and polycations through electrostatic interactions can generate colloidal polyelectrolyte complexes (PECs), which can offer a wide range of applications while being relatively simple to prepare with rapid and "green" processes. The advantages of colloidal HA-base...

show abstract

Polyelectrolyte Complexes between Hyperbranched and Linear Polysaccharides: Fucoidan/Chitosan

Gasilova,

Poshina,

Sitnikova

et al. 2023

Chin J Polym Sci

View full text Add to dashboard Cite

Diethylaminoethyl chitosan–hyaluronic acid polyelectrolyte complexes

Cited by 26 publications

References 47 publications

Electrospun Fibres with Hyaluronic Acid-Chitosan Nanoparticles Produced by a Portable Device

Electrospun Fibres with Hyaluronic Acid-Chitosan Nanoparticles Produced by a Portable Device

Colloidal Polyelectrolyte Complexes from Hyaluronic Acid: Preparation and Biomedical Applications

Polyelectrolyte Complexes between Hyperbranched and Linear Polysaccharides: Fucoidan/Chitosan

Contact Info

Product

Resources

About