Dietary vitamin A deficiency reduces growth performance, immune function of intestine, and alters tight junction proteins of intestine for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Liang, Dazhi; Yang, Qihui; Dong, Xiaohui; Chi, Shuyan; Liu, Hongyu; Zhang, Shuang

doi:10.1016/j.fsi.2020.10.016

Cited by 22 publications

(12 citation statements)

References 47 publications

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“…Fish samples were kept fast for 24 h before collection at the end of the trial and anesthetized with MS-222 (1:10,000) (Sigma, St Louis, MO, USA) (Wu et al, 2021). The fish samples of each experimental group were calculated and weighed, and it was determined that weight gain rate, survival rate and specific growth rate (Liang et al, 2020b). Afterward, three fish samples of each floating cage were randomly selected and then stored at -20°C to detect the whole-body composition (Wang et al, 2020a).…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

et al. 2022

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Glycerol monolaurate (GML), a representative fatty acid glyceride, was used to promote growth. This study investigated the effects of GML in diets on growth, serum biochemical parameters, antioxidant capacity, liver morphology and lipid metabolism for juvenile pompano Trachinotus ovatus. Six groups of T. ovatus (mean weight = 14.00 ± 0.70 g) were fed with six diets, containing varying GML levels of 0.00% (G0), 0.05% (G5), 0.10% (G10), 0.15% (G15), 0.20% (G20), and 0.25% (G25) for 8 weeks. Fish were reared for in floating cages (length: 1 m; width: 1 m; and height: 2 m). The highest weight gain rate (WGR), specific growth rate (SGR), and protein deposit rate (PDR) were observed in the G15 group (P < 0.05). Hepatic glutathione peroxidase, total antioxidant capacity and superoxide dismutase activities were significantly increased in the G10 and G15 groups compared with the G0 group (P < 0.05), whereas hepatic malondialdehyde (MDA) was significantly reduced in the G10 and G15 groups (P < 0.05). The alkaline phosphatase activity was significantly higher in the G5, G10, G15, and G20 groups than in the G0 group (P < 0.05), and the highest AKP activity was observed in the G15 group. Alanine aminotransferase (ALT) activity was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). The addition of GML to the feed significantly reduced triglyceride (TG) content (P < 0.05). In comparison with the G0 group, the G15, G20, and G25 groups had significantly lower total cholesterol content and significantly higher high-density lipoprotein content (P < 0.05). Low-density lipoprotein content was significantly lower in the G5 and G10 groups than in the G0 group (P < 0.05). Serious vacuolation occurred in the G0 group, but the cell boundaries in all added groups were obvious, and the rate of intracellular vacuolization decreased. As the GML level increased, carnitine palmitoyl-transferase Ι mRNA level was significantly up-regulated (P < 0.05). The expression of fatty acid synthesis in the G10, G15, G20, and G25 groups was significantly lower than that in the G0 group (P < 0.05). The expression of sterol regulatory element-binding protein-Ι was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). Based on the broken-line model of WGR and PDR corresponding to GML levels, the optimum addition level of dietary GML was 0.14%-0.16% for juvenile T. ovatus. In conclusion, 0.15% GML significantly improved the growth performance, hepatic antioxidant ability, and lipid metabolism and protected the liver for juvenile pompano T. ovatus.

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Section: Sample Collectionmentioning

confidence: 99%

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

et al. 2022

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“…In addition, the activation of the Nrf2-Keap1 signaling pathway affected by VA could weaken the proinflammatory cytokine production [81,101]. Similarly, VA's anti-inflammatory and immunity-stimulating role has also been reported in hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ [105] and grass carp, Ctenopharyngodon Idella [88]. The attenuation of proinflammatory response corresponds to the enhanced antioxidant system by VA supplementation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin A Supplementation on Growth Performance, Lipid Deposition, Antioxidant Ability, and Immunity in Juvenile Chinese Mitten Crab Eriocheir sinensis Fed Diet with Fish Oil Totally Replaced by Palm Oil

Huang

Wang

Liu

et al. 2022

Aquaculture Nutrition

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This research evaluated the protective effect of vitamin A (VA) on the adverse effect of fish oil (FO) substitution with palm oil (PO) in an economical crab Eriocheir sinensis. Three diets of FO, PO, and PO + 8000 IU / kg VA as the main lipid sources were fed to crabs, respectively, for 8 weeks. Compared to crabs fed FO diet, crabs fed PO diet showed reduced hemolymph VA concentration, feed utilization efficiency, and growth performance. Besides, crabs fed PO diet showed elevated lipid content in hepatopancreas and body and triglyceride content in hepatopancreas, leading to decreased antioxidant enzyme and immune parameters activities from biochemical analysis, enzymatic determination, and quantitative polymerase chain reaction. In contrast, compared to crabs fed PO only, VA supplementation in PO improved the growth performance and utilization of fatty acids and reduced lipid deposition in the hepatopancreas. In addition, VA supplementation suppressed gene expression related to triglyceride synthesis (dgat1) and positively affected gene expression related to lipid catabolism (cpt1a, cpt1b, cpt2, and caat). Furthermore, VA supplementation upregulated antioxidant genes (CuZnSOD and CAT) through downregulating gene expression of upstream regulator Keap1. Furthermore, VA supplementation upregulated immune genes (Lzm and proPO) expression and reduced proinflammatory genes (LITAF, ADAM17, and IL-16) expression related to Toll2/MyD88/Relish signaling pathway. This study shows the necessity of VA addition in the feed with FO totally replaced by PO because it can relieve PO’s adverse effects and improve the growth of crabs.

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“…The PCR primers were listed in Table 4. The PCR cycling protocol by Liang et al (2020b) was used, and all the real-time PCR reactions were performed on a Roche LightCycler480II (Switzerland) using an SYBR @ Premix Ex Taq TM Kit (Takara). The relative mRNA expressions were calculated using the 2 − CT method.…”

Section: Real-time Pcr Analysis Of the Organizationmentioning

confidence: 99%

A Challenge to Conventional Fish Meal: Effects of Soy Protein Peptides on Growth, Histomorphology, Lipid Metabolism and Intestinal Health for Juvenile Pompano Trachinotus ovatus

et al. 2022

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This experiment was conducted to assess the possibility of replacing fish meal (FM) with soy protein peptide (SPP) at different levels—0% (FM), 14.29% (S5), 28.57% (S10), 57.14% (S20), 71.42%(S25)—and its effects on growth, histology, gene expression related to liver lipid metabolism and intestinal immunity in juvenile pompano Trachinotus ovatus (initial mean weight = 39.88 ± 0.15 g). 600 healthy and uniformed-size fish were distributed to five groups of three replicates, each with 40 fish in each floating cage and fed twice daily for 8 weeks. Results showed that no significant difference in the growth was observed with SPP replacing FM (P > 0.05). Serum glutathione peroxidase activity in the S10 group was significantly higher than that in the FM group, and serum malondialdehyde content significantly decreased (P < 0.05). SPP significantly improved intestinal immunity by increasing alkaline phosphatase and lysozyme activities and up-regulating interleukin 10 and complement 4 mRNA levels while simultaneously decreasing triglyceride and total cholesterol content and down-regulating interleukin 1β mRNA expression. Villus length and muscle thickness in the S10 group were significantly higher than those in the FM group (P < 0.05). SPP significantly improved liver fat metabolism by increasing carnitine palmitoyl transferase I mRNA levels, and down-regulating fatty acid synthesis mRNA expression (P < 0.05). In summary, SPP substitution for FM promoted intestinal health, liver lipid metabolism and reduced liver fat accumulation for juvenile pompano T. ovatus, with no significant effect on growth performance. Based on the second-order polynomial analysis model of LYZ activity, the optimal replacement SPP level for juvenile pompano T. ovatus was 11.82%.

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Dietary vitamin A deficiency reduces growth performance, immune function of intestine, and alters tight junction proteins of intestine for juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)

Cited by 22 publications

References 47 publications

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

Effect of Vitamin A Supplementation on Growth Performance, Lipid Deposition, Antioxidant Ability, and Immunity in Juvenile Chinese Mitten Crab Eriocheir sinensis Fed Diet with Fish Oil Totally Replaced by Palm Oil

A Challenge to Conventional Fish Meal: Effects of Soy Protein Peptides on Growth, Histomorphology, Lipid Metabolism and Intestinal Health for Juvenile Pompano Trachinotus ovatus

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