Dietary synbiotic alters plasma biochemical parameters and fecal microbiota and metabolites in sows

Ma, Cui; Zhang, Wanghong; Gao, Qiankun; Zhu, Qian; Song, Mengya; Ding, Hao; Yin, Yulong; Kong, Xiangfeng

doi:10.1016/j.jff.2020.104221

Cited by 17 publications

(20 citation statements)

References 53 publications

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“…Our previous study showed that dietary synbiotic supplementation increased the piglet survival rate by improving the glycolipids absorption and utilization and altering the gut microbiota composition and abundances of sows (13). The present study hypothesizes that maternal synbiotic supplementation may modify beneficially blood indices, gut microbiota composition and metabolic activity, and the mucosal mRNA expression of genes related to the intestinal barrier function.…”

Section: Introductionmentioning

confidence: 53%

“…The V3-V4 regions was amplified using the primer 338F (5 ′ -GCACCTAA YTGGGYDTAAAGNG-3 ′ ) and 806R (5 ′ -TACNVGGGTATCTA ATCC-3 ′ ). The protocol of PCR amplification was conducted according to our previous study (13). The PCR products were successfully separated using 1.2% agarose gel electrophoresis, purified using Agencourt AMPure Beads (Beckman Coulter, Indianapolis, IN), and further quantified using the PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.…”

Section: Dna Extraction and 16s Rrna Gene Sequencingmentioning

confidence: 99%

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Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets

Gao

Zhang

et al. 2020

Front. Vet. Sci.

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Nutrients in the maternal diet favor the growth and development of suckling piglets and alter their gut microbiota composition and metabolic activity, thus affecting the hosts. The present study analyzed, in suckling piglets from sows receiving antibiotic or synbiotic supplements from pregnancy to lactation, several biochemical parameters, oxidative/anti-oxidative indices, inflammatory cytokines, and ingestion-related factor levels in plasma, as well as colonic microbiota composition and metabolic activity, and mucosal expression of genes related to the intestinal barrier function. Compared with the control group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, AMM, TC, low-density lipoprotein-cholesterol (LDL-C), MDA, H2O2, ghrelin, CCK, PP, IL-1β, IL-2, IL-6, TNF-α, Ala, Cys, Tau, and β-AiBA, the levels of propionate and total short-chain fatty acids (SCFAs) in the colonic luminal content, and colonic abundances of RFN20, Anaerostipes, and Butyricimonas; while increased (P < 0.05) the plasma levels of urea nitrogen (UN), Ile, Leu, α-AAA, α-ABA, and 1-Mehis, as well as colonic abundances of Sphingomonas, Anaerovorax, Sharpea, and Butyricicoccus. Compared with the antibiotic group, maternal synbiotic supplementation decreased (P < 0.05) the plasma levels of glucose, gastrin, and Ala, as well as abundances of Pasteurella and RFN20 and propionate level in the colonic content. Expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α were down-regulated in the colonic mucosa. The synbiotic supplementation increased (P < 0.05) the plasma levels of UN, Leu, α-ABA, and 1-Mehis, the abundances of Anaerovorax, Sharpea, and Butyricicoccus and expression of genes coding for E-cadherin, Occludin, ZO-1, ZO-2, IL-10, and interferon-α. Spearman correlation analysis showed that there was a positive correlation between colonic Anaerostipes abundance and acetate and SCFAs levels; whereas a negative correlation between Fusobacteria and Fusobacterium abundances and acetate level. These findings suggest that synbiotic supplementation in the maternal diet improved nutrient metabolism and intestinal barrier permeability, reduced oxidative stress, and modified colonic microbiota composition and metabolic activity in suckling piglets.

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Section: Introductionmentioning

confidence: 53%

Section: Dna Extraction and 16s Rrna Gene Sequencingmentioning

confidence: 99%

Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets

Gao

Zhang

et al. 2020

Front. Vet. Sci.

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“…In addition, dietary supplementation with Bacillus subtilis C-3102 to sows during gestation and lactation periods and to progeny after weaning modified the fecal microbiota population in sows and nursery piglets (13). Our previous studies also demonstrated that dietary supplementation with compound probiotics (L. Plantarum B90 and S. cerevisiae P11) or synbiotics (compound probiotics + XOS) during pregnancy and lactation periods can improve piglet's survival and lipid metabolism by altering gut microbiota diversity and composition (14,15). However, the long-term effect of providing probiotics or synbiotics to sows and their offspring on the colonic microbiota, antioxidant capacity, and immune function in weaned piglets is still unclear.…”

Section: Introductionmentioning

confidence: 95%

Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets

et al. 2020

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This study was conducted to investigate the effect of dietary probiotics or synbiotics supplementation on colonic microbiota, antioxidant capacity, and immune function in weaned piglets. A total of 64 pregnant Bama mini-sows and then 128 of their weaned piglets were randomly assigned into control group, antibiotics group, probiotics group, or synbiotics group. The results showed that colonic Firmicutes and Bifidobacterium abundances in the probiotics group and total bacteria, Bacteroidetes, and Lactobacillus abundances in the synbiotics group were increased (P < 0.05), while Escherichia coli abundance in the synbiotics group was decreased (P = 0.061) compared with the control group. Firmicutes, Bifidobacterium, and total bacteria abundances were increased (P < 0.05) in the probiotics and synbiotics groups compared with the antibiotics group. Probiotics supplementation up-regulated (P < 0.05) the mRNA expression of GPR109A compared with the control and antibiotics groups. Dietary probiotics or synbiotics supplementation improved the antioxidant capacity by increasing (P < 0.05) the colonic CAT, GSH-Px, SOD, and T-AOC levels and plasma CAT, GSH, GSH-Px, and SOD levels and by decreasing (P < 0.05) the colonic and plasma MDA and H2O2 levels. Compared to the control group, the colonic IL-10, IFN-α, and sIgA concentrations and plasma IgA and IgM concentrations were significantly increased (P < 0.05) in the probiotics and synbiotics groups. Spearman's correlation analysis showed that the changed colonic microbiota, such as Lactobacillus and Bifidobacterium were correlated with the alteration of antioxidant indexes, cytokines, and immunoglobulins. In conclusion, dietary probiotics or synbiotics supplementation during gestation, lactation, and nursery periods could be used as an alternative for antibiotics in terms of gut health of weaned piglets.

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“…Our previous study has found that dietary synbiotic supplementation to sows during pregnancy and lactation can improve piglet's survival and lipid metabolism by altering gut microbiota diversity and composition [ 21 ]. Therefore, we hypothesized that maternal probiotic or synbiotic supplementation could improve the antioxidant capacity and mitochondrial function by altering the gut microbiota of offspring weaned piglets.…”

Section: Introductionmentioning

confidence: 99%

Maternal Probiotic or Synbiotic Supplementation Modulates Jejunal and Colonic Antioxidant Capacity, Mitochondrial Function, and Microbial Abundance in Bama Mini‐piglets

Wang

Kong

Azad

et al. 2021

Oxidative Medicine and Cellular Longevity

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The present study was conducted to investigate the effects of maternal probiotic or synbiotic supplementation during gestation and lactation on antioxidant capacity, mitochondrial function, and intestinal microbiota abundance in offspring weaned piglets. A total of 64 pregnant Bama mini-sows were randomly allocated into the control group (basal diet), antibiotic group ( basal diet + 50 g/t virginiamycin), probiotic group ( basal diet + 200 mL/d probiotics per pig), or synbiotic group ( basal diet + 200 mL/d probiotics per pig + 500 g/t xylo-oligosaccharides). On day 30 of post-weaning, eight piglets per group with average body weight were selected for sample collection. The results showed that maternal probiotic supplementation increased the catalase (CAT) activity in plasma and glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities in plasma, jejunum, and colon of piglets while decreased the malondialdehyde (MDA) and H2O2 concentrations in plasma compared with the control group ( P < 0.05 ). Moreover, maternal synbiotic supplementation increased the plasma CAT activity, jejunal glutathione and GSH-Px activities, jejunal and colonic total antioxidant capacity activity, and plasma and colonic SOD activity while decreased the colonic MDA concentration of offspring piglets compared with the control group ( P < 0.05 ). The mRNA levels of antioxidant enzyme-related genes (copper- and zinc-containing superoxide dismutase, nuclear factor erythroid 2-related factor 1, and nuclear factor erythroid 2-related factor 2) and mitochondrial-related genes (adenosine triphosphate synthase alpha subunit, adenosine triphosphate synthase β, and mitochondrial transcription factor A) in the jejunal mucosa were significantly upregulated, while the level of colonic peroxisome proliferator-activated receptor γ coactivator-1α was downregulated by maternal synbiotic supplementation ( P < 0.05 ). Maternal probiotic supplementation increased ( P < 0.05 ) the Bacteroidetes abundance in the jejunum and Bifidobacterium abundance in the jejunum and colon, and synbiotic supplementation increased ( P < 0.05 ) the abundances of Firmicutes, Bacteroidetes, Bifidobacterium, and Lactobacillus in the jejunum of piglets. Furthermore, correlation analysis revealed that intestinal microbiota abundances were significantly correlated with antioxidant enzyme activities and mitochondrial-related indexes. These findings indicated that maternal probiotic or synbiotic supplementation might be a promising strategy to improve the antioxidant capacity and mitochondrial function of offspring weaned piglets by altering the intestinal microbiota.

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Dietary synbiotic alters plasma biochemical parameters and fecal microbiota and metabolites in sows

Cited by 17 publications

References 53 publications

Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets

Supplementing Synbiotic in Sows' Diets Modifies Beneficially Blood Parameters and Colonic Microbiota Composition and Metabolic Activity in Suckling Piglets

Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets

Maternal Probiotic or Synbiotic Supplementation Modulates Jejunal and Colonic Antioxidant Capacity, Mitochondrial Function, and Microbial Abundance in Bama Mini‐piglets

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