Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia ( Oreochromis niloticus )

Saputra, Febriyansyah; Shiu, Ya‐Li; Chen, Yo-Chia; Puspitasari, Asthervina Widyastami; Danata, Ridha Handriany; Liu, Chun-Hung; Hu, Shao‐Yang

doi:10.1016/j.fsi.2016.09.046

Cited by 66 publications

(32 citation statements)

References 39 publications

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“…Aeromonas hydrophila is a well-known pathogen that generally causes disease in Nile tilapia [23]. The pathogenic bacterium, A. hydrophila KCTC2358, was received from the Department of Biotechnology, Pukyong National University, Busan, Republic of Korea.…”

Section: Challenge Testmentioning

confidence: 99%

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

et al. 2020

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An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), Bacillus subtilis at 107 (BS7) and at 108 CFU/g diet (BS8), Lactococcus lactis at 107 CFU/g (LL7) and at 108 CFU/g diet (LL8), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 ± 0.05 g (mean ± SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS8, LL8 and LL7 diets were significantly higher than those of fish fed CON diet (p < 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS8, LL8, BS7, LL7 and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS8, LL8 and LL7 diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1β), interferon-gamma (IFN-γ) and tumour necrosis factor (TNF-α) gene expression of fish fed BS8 and LL8 diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS8 and LL8 diets were significantly higher than those of fish fed CON, BS7 and OTC diets. Based on these results, B. subtilis and L. lactis at 108 (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia.

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Section: Challenge Testmentioning

confidence: 99%

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

et al. 2020

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“…are considered a safe and friendly bacterium for aquaculture because of its probiotic characteristics (Leonel Ochoa‐Solano & Olmos‐Soto, ; Liu et al, , , , ; Saputra et al, ; Tseng et al, ). Bacillus enzymes are highly efficient at breaking down a large variety of carbohydrates, lipids and proteins into smaller units to facilitate digestion and growth in farmed animals (Inatsu et al, ; Leonel Ochoa‐Solano & Olmos‐Soto, ; Liu et al, ; Saputra et al, ; Tari, Genckal, & Tokatlı, ). B. subtilis E20 is known to improve growth in aquatic animals (Huang et al, ; Liu et al, , , , ; Shiu, Hsieh, et al, ; Tseng et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Jian); similar results were observed in Japanese seabass ( Lateolabrax japonicus ; Ai et al, ). Saputra et al () indicated that dietary supplementation with xylanase‐expressing B. amyloliquefaciens R8 facilitated growth and enhanced immunity in Nile tilapia. In addition, Cheng et al () showed that the addition of X‐xylanase (Hostazym ® , Huvepharma) to a tilapia diet improved feed utilization and consequently enhanced growth among the fish.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the use of exogenous carbohydrases in aquafeed has attracted attention. Multiple responses have addressed the use of exogenous carbohydrases as supplements to improve feed intake, improve growth and nutrient digestibility, and enhance immunity (Castillo & Gatlin, ; Cheng, Chi, & Liu, ; Goda, Mabrouk, Wafa, & El‐Afifi, ; Jiang et al, ; Lin, Mai, & Tan, ; Saputra et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Secretory expression and characterization of xylanase isolated from Bacillus subtilis E20 increase the utilization of plant ingredients in tilapia feed

Tsai

Wang

et al. 2019

Aquac Res

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Bacillus subtilis E20, an isolate from natto – a Japanese fermented soy product – is used in a wide variety of marine and freshwater aquatic species to enhance growth and immunity. This paper describes the cloning, expression and characterization of xylanase isolated from B. subtilis E20 in B. subtilis RIK1285. The BsXynE20 gene was identified to encode a 213‐amino‐acid protein for a glycoside hydrolase family 11 xylanase containing a 28‐residue signal peptide whose cleavage yields a 185‐residue mature protein with a predicted molecular weight of 20.25 kDa. The full length of the BsXynE20 gene was subcloned into the pBE‐S expression vector for secretory production of recombinant BsXynE20 in B. subtilis RIK1285. Western blot and zymogram analysis, respectively, revealed that recombinant BsXynE20 can be secreted into culture medium and is a functional xylanase. The xylanase exhibited optimal activity at pH 5.0–6.0 and 60°C. Using recombinant BsXynE20‐pretreated duckweed meal for feed preparation improved the growth performance of tilapia. Finally, we achieved secretive expression of functional BsXynE20, which can improve the nutrient utilization of a plant‐derived diet. Thus, the use of BsXynE20 may be beneficial for tilapia aquaculture.

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“…Transformants carrying different numbers of copies of xynB were separately inoculated into galactose medium in a fermenter and incubated at 30 °C for 72 h with an agitation speed of 150 rpm. Xylanase activity in the resulting cultures was determined using the DNS method as described previously [18] .…”

Section: Fermentation Of Transformants To Obtain Xylanasementioning

confidence: 99%

Expression and function of an HAC1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae

Zhang

Bao

et al. 2020

Preprint

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To investigate the effects of transcription factor HAC1, which is involved in the unfolded protein response pathway, on the expression of xynB in Saccharomyces cerevisiae , we used overlap extension polymerase chain reaction (PCR), rDNA integration, and droplet digital PCR technology to generate a S . cerevisiae strain (S8) containing eight copies of the xylanase gene, allowing high-yield secretory expression of xylanase. HAC1 was then overexpressed in the S8 strain, and the GeXP system was used to study the effects of HAC1 overexpression on the expression of genes associated with protein folding in the endoplasmic reticulum (ER). Results confirmed the constitutive secretory expression of the multiple copies of the xylanase gene in S . cerevisiae following rDNA-based integration of the expression cassette. Specifically, recombinant S . cerevisiae strain S8, containing eight copies of the xylanase gene, showed maximum xylanase expression, with a yield of 325 U/mL. However, overexpression of HAC1 further improved xylanase production by strain S8, resulting in a yield of 381 U/mL. These results confirmed that overexpression of HAC1 improves the expression of genes associated with protein folding in the ER, enhancing the protein folding and assembly functions of the ER and increasing xylanase expression.

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Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia ( Oreochromis niloticus )

Cited by 66 publications

References 39 publications

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Secretory expression and characterization of xylanase isolated from Bacillus subtilis E20 increase the utilization of plant ingredients in tilapia feed

Expression and function of an HAC1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae

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Dietary supplementation with xylanase-expressing B. amyloliquefaciens R8 improves growth performance and enhances immunity against Aeromonas hydrophila in Nile tilapia ( Oreochromis niloticus )

Cited by 66 publications

References 39 publications

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Secretory expression and characterization of xylanase isolated from Bacillus subtilis E20 increase the utilization of plant ingredients in tilapia feed

Expression and function of&nbsp;an HAC1-regulated multi-copy xylanase&nbsp;gene in Saccharomyces cerevisiae

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Expression and function of an HAC1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae