Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets1

Abstract

Weaning is associated with reduced intestinal absorptive capacity in piglets. Our previous study indicated that dietary supplementation with N-carbamylglutamate (NCG) enhanced growth performance and improved intestinal function in weaned piglets. The present study was conducted to test the hypothesis that dietary supplementation with NCG may increase the growth performance of weaned piglets by regulating the expression of intestinal nutrient transporters, thus enhancing nutrient absorption. Twenty-four Huanjiang mini-pig piglets weaned at 21 d of age (3.17 ± 0.21 kg average BW) were randomly assigned to 2 dietary treatments consisting of a basal diet and the basal diet with 0.1% NCG supplementation for a 14-d period with 6 pens per treatment and 1 male and 1 female per pen. On d 14, 1 piglet was randomly selected from each pen for blood and tissue sampling. Dietary NCG supplementation enhanced (P < 0.05) growth rate and the efficiency of feed use in weaned Huanjiang mini-pig piglets. The NCG-supplemented diet increased (P < 0.05) mRNA expression levels of Slc6a19, Slc7a9, and Slc1a1 and the protein abundance of ASCT2, B(0)AT1, b(0,+)AT, y(+)LAT1, and EAAC1 in the jejunum. Furthermore, the contents of low density lipoprotein, ammonia, urea nitrogen, and AA as well as the activity of alkaline phosphatase in plasma were all altered (P < 0.05) by supplementation with NCG. These findings indicate that dietary supplementation with NCG may improve intestinal absorptive function in weaned piglets by increasing the expression of AA transporters in the intestine.

Maternal N-Carbamylglutamate Supplementation during Early Pregnancy Enhances Embryonic Survival and Development through Modulation of the Endometrial Proteome in Gilts

“…Large increases in mRNA levels for EF1Awere observed in rapidly proliferating cells and embryos, which indicate that the level of expression of EF1A correlates with the rate of cell growth and proliferation (42). Previous studies have shown that NCG supplementation enhances protein synthesis in the skeletal muscle of piglets (43) and improves the intestinal absorptive function in weaned piglets by increasing the expression of amino acid transporters in the intestine (44). An adequate endometrial nutrient metabolism is an essential part of endometrial differentiation and decidualization FIGURE 1 The relative mRNA expression in the endometrium tissue on days 14 (A) and 28 (B) of gestating gilts fed a basal diet that was or was not supplemented with 0.05% NCG from days 0 to 28 of gestation.…”

Energy metabolism in intestinal epithelial cells during maturation along the crypt-villus axis

“…Western blot analysis was performed as previously described35. The denatured proteins of cell fractions were separated using SDS-PAGE (10% gradient gel), and transferred to PVDF membranes (Millipore, Billerica, MA) overnight at 12 V using a Bio-Rad Transblot apparatus (Hercules, CA).…”

Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

“…Bama mini-pigs had higher (P < 0.05) mRNA levels for LAT1, SNAT2, and EAAC1, but a lower (P < 0.05) mRNA level for PepT1, compared with Landrace pigs. Collectively, our synthesis in cells and signaling molecules (Wu et al , 2012(Wu et al , b, 2015Li et al 2011;Wang et al 2014aWang et al , b, 2015a but also regulators of gene expression (He et al 2013;Chen et al 2014;Zhang et al 2013;Ren et al 2013aRen et al , b, c, 2014aTan et al 2011;Yang et al 2013;Feng et al 2014;Tang et al 2014Kong et al 2014) and protein posttranslational regulation . Additionally, AAs are key precursors for the biosynthesis of hormones and low-molecular-weight nitrogenous substances (Wu 2013a, b).…”