Dietary supplementation with metformin improves testis function and semen quality and increases antioxidants and autophagy capacity in goats

Zhao, Jie; Yang, Pengcheng; Yang, Hua; Wang, Zhi-bo; El-Samahy, M.A.; Wang, Rui

doi:10.1016/j.theriogenology.2022.05.030

Cited by 5 publications

(3 citation statements)

References 69 publications

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“…Despite this, its potential role in enhancing reproductive performance in female livestock animals has not been evaluated, so far. To date, investigations into the bene ts of metformin in animal reproduction remain limited, with only a small number of studies examining its effects on sperm kinematics in boar(Li et al 2020), dog (Grandhaye et al 2020), and buck(Zhao et al 2022). Though, we have not observed any untoward effect of metformin, yet the data are still insu cient to completely con rm or disprove negative effects of metformin.…”

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confidence: 60%

A novel action of insulin sensitizing drug as a potential promotor of preovulatory follicles, ovulation rate and prolificacy in sheep

Kumawat,

Kumar,

Mahla

et al. 2023

Vet Res Commun

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Impact of insulin-sensitizing drug metformin on preovulatory follicle (POF) turnover, ovulation rate, and proli cacy was investigated in forty-six cyclic Malpura ewes. Following estrus synchronization, the ewes were equally divided into two groups (n = 23). Treatment group (MET) received a daily oral dose of metformin at the rate of 500 mg/animal for around 12 wk, spanning ve estrous cycles, as against untreated control (CON). All the ewes were bred with proven sires at the end of treatment. Ultrasonographic ovarian scans were carried out on every estrus and D 9 of each cycle to evaluate the number and diameter of POFs and corpora lutea (CL), respectively. A comprehensive assessment was conducted on circulating hormones including, estradiol, progesterone, androstenedione, and insulin as well as metabolic indicators like glucose, and lipid pro le parameters. By the end of medication, the treatment showed a stimulatory effect on follicular turnover with a 53.2% (P < 0.001) rise in the number of POFs. It further elevated the ovulation rate by 67.4% (P < 0.01), with a higher proportion (χ 2 df1 = 10.7, P < 0.001) of ewes in MET group having multiple ovulations compared to the CON (82.6 vs. 30.4%, respectively). The proportion of ewes delivering multiple lambs was 2.9-fold higher in MET group than the CON. The plasma estradiol, insulin, glucose, total cholesterol, and LDL-cholesterol concentrations were lower (P < 0.05) in the MET ewes than in the CON. The ndings of the present study indicate that metformin can augment POF numbers, ovulation rate, and proli cacy in ewe concurrent with reduced plasma estradiol, insulin, glucose and cholesterols in MET ewes.

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confidence: 60%

A novel action of insulin sensitizing drug as a potential promotor of preovulatory follicles, ovulation rate and prolificacy in sheep

Kumawat,

Kumar,

Mahla

et al. 2023

Vet Res Commun

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“…RNA-Seq methods have been broadly utilized to determine DEGs or gene expression patterns, new transcripts, AS events and SNPS, and have empowered studies examining porcine [24,25], cattle [26,27] and mouse [28,29] testicular development. In goats, the profiles of ovarian [30,31], uterine [32,33] and testicular [34,35] tissues under different conditions were recently compared by RNA-Seq. However, limited data on testicular development in goats were available.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of the Developing Testis and Spermatogenesis in Qianbei Ma Goats

Zou,

Chen,

Tian

et al. 2023

Genes

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Reproductive competence in male mammals depends on testicular function. Testicular development and spermatogenesis in goats involve highly complex physiological processes. In this study, six testes were, respectively, obtained from each age group, immature (1 month), sexually mature (6 months) and physically mature (12 months old) Qianbei Ma goats. RNA-Seq was performed to assess testicular mRNA expression in Qianbei Ma goats at different developmental stages. Totally, 18 libraries were constructed to screen genes and pathways involved in testis development and spermatogenesis. Totally, 9724 upregulated and 4153 downregulated DEGs were found between immature (I) and sexually mature (S) samples; 7 upregulated and 3 downregulated DEGs were found between sexually mature (S) and physically mature (P) samples, and about 4% of the DEGs underwent alternative splicing events between I and S. Select genes were assessed by qRT-PCR, corroborating RNA-Seq findings. The detected genes have key roles in multiple developmental stages of goat testicular development and spermatogenesis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to determine differentially expressed genes (DEGs). GO analysis revealed DEGs between S and P contributed to “reproduction process”, “channel activity” and “cell periphery part” between I and S, and in “ion transport process”, “channel activity” and “transporter complex part”. KEGG analysis suggested the involvement of “glycerolipid metabolism”, “steroid hormone biosynthesis” and “MAPK signaling pathway” in testis development and spermatogenesis. Genes including IGF1, TGFB1, TGFBR1 and EGFR may control the development of the testis from immature to sexually mature, which might be important candidate genes for the development of goat testis. The current study provides novel insights into goat testicular development and spermatogenesis.

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“…With the rapid development of next-generation sequencing, RNA sequencing (RNA-Seq) provides researchers with a powerful toolbox for characterisation and quantification of the transcriptome and is widely used in studying the regulation of gene expression [ 13 ]. For instance, Zhao et al [ 14 ] identified differentially expressed genes (DEGs) between goats treated with and without the antioxidant metformin using RNA-Seq technology and found that metformin regulates testis function, semen quality, antioxidant levels, and autophagy capacity in goats. Moreover, Cheng et al [ 15 ] found that the antioxidant capacity of young ruminants acts as an indicator of health; they performed Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis of their RNA-Seq results and found that the upregulated DEGs are mainly related to the ECM-receptor interaction and axon guidance and that the downregulated DEGs are mainly related to arachidonic acid metabolism, complement and coagulation cascades, and alpha-linolenic acid metabolism in neonatal goats with diarrhoea compared with control goats.…”

Section: Introductionmentioning

confidence: 99%

Differentially Expressed Genes and Signalling Pathways Regulated by High Selenium Involved in Antioxidant and Immune Functions of Goats Based on Transcriptome Sequencing

Wang

Ban

et al. 2023

IJMS

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The objective of this study is to observe the effect of high selenium on the antioxidant and immune functions of growing goats based on transcriptome sequencing. Eighteen goats were randomly divided into three groups: (1) the control (CON) group was fed a basal diet, and (2) the treatment 1 group (LS) and treatment 2 group (HS) were fed a basal diet with 2.4 and 4.8 mg/kg selenium-yeast (SY), respectively. The results indicate that HS treatment significantly (p < 0.05) increased the apparent digestibility of either extract and significantly increased (p < 0.05) total antioxidant capacity, whereas it significantly (p < 0.05) decreased plasma aspartate aminotransferase and malondialdehyde relative to the control group. The LS treatment had significantly (p < 0.05) increased glutathione S-transferase and catalase compared to CON. A total of 532 differentially expressed genes (DEGs) between the CON and HS were obtained using transcriptome sequencing. Kyoto Encyclopedia of Genes and Genomes analysis identified upregulated (p < 0.05) DEGs mainly related to vascular smooth muscle contraction, alpha-linolenic acid metabolism, biosynthesis of unsaturated fatty acids, the VEGF signalling pathway, and proteoglycans in cancer; downregulated (p < 0.05) DEGs mainly related to the NOD-like receptor signalling pathway, influenza A, cytokine-cytokine receptor interaction, haematopoietic cell lineage, and African trypanosomiasis. Ontology analyses of the top genes show that the identified DEGs are mainly involved in the regulation of granulocyte macrophage colony-stimulating factor production for biological processes, the external side of the plasma membrane for cellular components, and carbohydrate derivative binding for molecular functions. Seven genes are considered potential candidate genes for regulating antioxidant activity, including selenoprotein W, 1, glutathione peroxidase 1, glutathione S-transferase A1, tumour necrosis factor, tumour necrosis factor superfamily member 10, tumour necrosis factor superfamily member 8, and tumour necrosis factor superfamily member 13b. The experimental observations indicate that dietary supplementation with 4.8 mg/kg SY can enhance antioxidant and immune functions by improving muscle immunity, reducing the concentrations of inflammatory molecules, and modulating antioxidant and inflammatory signalling pathways in growing goats.

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Dietary supplementation with metformin improves testis function and semen quality and increases antioxidants and autophagy capacity in goats

Cited by 5 publications

References 69 publications

A novel action of insulin sensitizing drug as a potential promotor of preovulatory follicles, ovulation rate and prolificacy in sheep

A novel action of insulin sensitizing drug as a potential promotor of preovulatory follicles, ovulation rate and prolificacy in sheep

Transcriptomic Analysis of the Developing Testis and Spermatogenesis in Qianbei Ma Goats

Differentially Expressed Genes and Signalling Pathways Regulated by High Selenium Involved in Antioxidant and Immune Functions of Goats Based on Transcriptome Sequencing

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