Dietary Supplementation with Lactic Acid Bacteria Attenuates the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice in a Strain-Dependent Manner

Abstract

Background: Dietary supplementation with lactic acid bacteria (LAB) is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis (AD). However, the influence of different bacterial strains and their immunomodulating capacities is still largely unknown. Methods: AD-like skin lesions were induced by sensitization to and repeated challenges with picrylchloride in the Th2-skewed NC/Nga mouse strain. The effects of LAB supplementation were assessed over time by monitoring clinical scores and plasma IgE levels. In some cases, mast cell infiltration, cutaneous hypersensitivity responses and cytokine mRNA expression in auricles were also examined. Additionally, cytokine production in vitro and cytokine mRNA accumulation in major lymphoid tissues were measured, comparing Lactobacillus paracasei KW3110 with L. rhamnosus GG (LGG). Results: Supplementation with KW3110 significantly reduced the development of AD-like skin lesions, accompanied by less mast cell infiltration and lower plasma IgE levels. KW3110 also suppressed immediate hypersensitivity reactions and IL-4 mRNA expression in the auricles. These preventive effects sustained when supplementation was terminated; moreover, inhibitory effects were also observed even when supplementation was initiated after the onset of symptoms. In accordance with its effects on IL-12 and IL-4 production in vitro, KW3110 prevented the emergence of clinical symptoms more effectively than LGG in vivo. Conclusions: Supplementation with KW3110 significantly attenuated the onset and exacerbation of AD-like symptoms in NC/Nga mice. The effects were more prominent than those obtained with LGG, suggesting the importance of differences between LAB strains and their immunomodulating capacity.

RETRACTED: Preventative and Therapeutic Role of Probiotics in Various Allergic and Autoimmune Disorders: An Up-to-Date Literature Review of Essential Experimental and Clinical Data

“…61 Also, supplementation with KW3110 strain of lactic acid bacteria significantly attenuated the onset and exacerbation of atopic dermatitis-like skin lesions, accompanied by lower plasma IgE levels through its effects on IL-12 and IL-4 production in vitro. 62 Oral administration of Lactobacillus brevis significantly inhibited IgE production and ear swelling, and suppressed the development of dermatitis in a dose-dependent manner in atopic dermatitis model of NC/Nga mice. Cytokines, for example, IL-10 and TGF-b production from Peyer's patch cells significantly increased in the treatment group.…”

Orally AdministeredLactobacillus paracaseiKW3110 Inducesin VivoIL-12 Production

“…Indeed, we have reported that oral administration of KW3110 attenuates the development of atopic-dermatitis-like skin lesions in NC/Nga mice. 24) There are reports indicating IL-12 independent anti-allergic mechanisms of LAB. Kanzato et al suggested the induction of antigen specific apoptosis by oral administration of LAB.…”

Oral Administration of Lactic Acid Bacteria Isolated from Traditional South Asian Fermented Milk `Dahi' Inhibits the Development of Atopic Dermatitis in NC/Nga Mice

“…On the other hand, Segawa et al reported that there was a high correlation between clinical score and serum total IgE level while oral administration of heat-treated LAB (Lactobacillus brevis SBC8803) significantly prevented the development of AD and serum total IgE elevation in NC/ Nga mice (35). Wakabayashi et al reported that the increase in total IgE levels was slower than the rise in clinical score while oral administration of heat-treated LAB (Lactobacillus paracasei KW3110) significantly prevented the development of AD and serum total IgE elevation in NC/Nga mice (36). In our study, it would appear that the clinical score did not correlate well with serum total IgE level.…”