Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide

Abstract

This study was to evaluate the protective roles of L-glutamate (Glu) and L-aspartate (Asp) in weaned piglets challenged with H2O2. Forty weaned piglets were assigned randomly into one of five groups (8 piglets/group): (1) control group (NC) in which pigs were fed a corn- and soybean meal-based diet and received intraperitoneal administration of saline; (2) H2O2 group (PC) in which pigs were fed the basal diet and received intraperitoneal administration of 10 % H2O2 (1 ml/kg body weight once on days 8 and repeated on day 11); (3) PC + Glu group (PG) in which pigs were fed the basal diet supplemented with 2.0 % Glu before intraperitoneal administration of 10 % H2O2; (4) PC + Asp group (PA) in which pigs were fed the basal diet supplemented with 1.0 % Asp before intraperitoneal administration of 10 % H2O2; (5) PC + Glu + Asp group (PGA) in which pigs were fed the basal diet supplemented with 2.0 % Glu plus 1.0 % Asp before intraperitoneal administration of 10 % H2O2. Measured parameters included daily feed intake (DFI), average daily gain (ADG), feed conversion rate (FCR), and serum anti-oxidative enzyme activities (catalase, superoxide dismutase, glutathione peroxidase-1), serum malondialdehyde and H2O2 concentrations, serum amino acid (AA) profiles, and intestinal expression of AA transporters. Dietary supplementation with Glu, Asp or their combination attenuated the decreases in DFI, ADG and feed efficiency, the increase in oxidative stress, the alterations of serum AA concentrations, and the changed expression of intestinal AA transporters in H2O2-challenged piglets. Thus, dietary supplementation with Glu or Asp alleviates growth suppression and oxidative stress, while restoring serum the amino acid pool in H2O2-challenged piglets.

Serine alleviates oxidative stress via supporting glutathione synthesis and methionine cycle in mice

“…Recently, our colleagues found that plasma serine concentration was significantly decreased after the pigs were treated with diquat [4,5], which indicated that serine deficiency also existed under oxidative stress in a pig model. And they suggested that glutamate, which is also an indirect precursor of GSH, could alleviate oxidative stress by improving GSH synthesis.…”

N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function

“…Intragastric or intraperitoneal administration of H 2 O 2 could decrease growth performance and caused oxidative stress [21–23]. Furthermore, previous studies showed that addition of H 2 O 2 (300  μ M) to chicken intestinal epithelial cells for 24 h significantly decreased cell survival and SOD activity [24].…”

Nutritional Composition of Apis mellifera Drones from Korea and Denmark as a Potential Sustainable Alternative Food Source: Comparison Between Developmental Stages

“…Adult drones exhibited the highest antioxidant activity followed by pupae and larvae, which is presumably due to the presence of high amounts of polyphenols. Furthermore, high amounts of amino acids like aspartic acid, glutamic acid, cysteine and lysine can improve the consequences of oxidative stress [76,77]. The gradual increase in their amount along with the developmental stages of the drones can justify the higher antioxidant activity of adults.…”