Dietary supplementation with <i>Clostridium butyricum</i>
 helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic <i>Escherichia coli</i>
 K88

Li, Haihua; Li, Yupeng; Zhu, Qi; Qiao, Jie; Wang, Wenjie

doi:10.1111/jam.13936

Cited by 51 publications

(59 citation statements)

References 34 publications

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“…The trillions of intestinal microbes harboured in the mammalian gut profoundly influences the host’s health and disease status (Vaarala ; Frese et al ; Lin et al ). An increasing number of studies have shown that the small intestinal microbiota has a profound impact on host metabolism and growth (El Aidy et al ; Burbach et al ; Li et al ). The present study characterized the small intestinal microbiota profiles of newborn piglets with IUGR.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the microbial community in the small intestine has a lower diversity and abundance than the colonic microbiota (Donaldson et al ). Li et al () showed that low birth weight piglets have a different faecal microbial community structure and metabolite profiles, suggesting the gut microbial potentially associated with the impaired growth and development of these piglets. However, the composition of the small intestinal microbiota in IUGR piglets still remains unknown.…”

Section: Introductionmentioning

confidence: 99%

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Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances

Zhang

Xie

et al. 2019

J of Applied Microbiology

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Aim Intrauterine growth retardation (IUGR) is a prevalent problem in mammals. The present study was conducted to unveil the alterations in intestinal microbiota in IUGR piglets. Methods and Results We identified the alterations of small intestinal microbiota in IUGR piglets on 7, 21 and 28 days of age using 16S rRNA sequencing. The results showed that IUGR piglets had a decreased alpha diversity of jejunum microbiota at 7 and 21 days of age; had lower abundances of Bacteroidetes and Bacteroides in the jejunum at 7, 21 and 28 days of age, Oscillibacter in the jejunum at 21 days of age, and Firmicutes in the ileum at 21 days of age; whereas they had higher abundances of Proteobacteria and Pasteurella in the ileum at 21 days of age and Escherichia–Shigella in the jejunum at 28 days of age. Correlation analysis showed that Bacteroides, Oscillibacter and Ruminococcaceae_UCG‐002 compositions were positively associated with the body weight (BW) of IUGR piglets, nevertheless Proteobacteria and Escherichia–Shigella relative abundances were negatively correlated with the BW of IUGR piglets. Gene function prediction analysis indicated that microbiota‐associated carbohydrate metabolism, lipid metabolism, glycan biosynthesis and metabolism, amino acid metabolism, and xenobiotics biodegradation and metabolism were downregulated in the IUGR piglets compared to control piglets. Conclusions The present study profiled the intestinal microbiota of newborn piglets with IUGR and the newborn IUGR piglets have lower diversity and different taxonomic abundances. Alterations in the abundances of Bacteroidetes, Bacteroides, Proteobacteria Escherichia–Shigella and Pasteurella may be involved in nutrient digestion and absorption, as well as the potential mechanisms connecting to the growth and development of IUGR in mammals. Significance and Impact of the Study The small intestinal microbiota were highly shaped in the IUGR piglets, which might further mediate the growth and development of IUGR piglets; and the gut microbiota could serve as a potential target for IUGR treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances

Zhang

Xie

et al. 2019

J of Applied Microbiology

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“…Saccharomyces boulardii (SB) is beneficial for gut health [14][15][16] and could reduce the abundance of intestinal aerobic bacteria [17,18]. Clostridium butyricum (CB) is known as a regulator for gut health, and the dietary supplementation of CB can improve early intestinal barrier function [19][20][21][22]. However, whether SB or/and CB could improve the effect of early intervention remains elusive.…”

Section: Introductionmentioning

confidence: 99%

Early Intervention Using Fecal Microbiota Transplantation Combined with Probiotics Influence the Growth Performance, Diarrhea, and Intestinal Barrier Function of Piglets

Xiang

Pan

et al. 2020

Applied Sciences

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Early intervention with fecal microbiota transplantation (FMT) improves the growth performance and intestinal barrier function of piglets. Accelerating intestinal oxygen concentration is beneficial for symbiotic bacterial colonization. Saccharomyces boulardii (SB) is an aerobic fungus, which may contribute to the colonization of anaerobic symbiotic bacteria by competing for oxygen. Clostridium butyricum (CB) improves intestinal barrier function and performance, via regulating the gut microbiota composition of piglets. The objective of this study was to investigate the effect of early intervention with FMT combining CB and SB on growth performance, diarrhea, and intestinal barrier function in piglets. A total of 77 litters of neonatal piglets assigned to one of six treatments, which treated with antibiotics (AB), placebo (CON), and FMT (FMT), FMT-added CB (FMT+C), FMT-added SB (FMT+S), and FMT-added CB and SB (FMT+C+S), respectively. FMT+C+S treated piglets had higher body weight (BW) and average daily gain (ADG) both in weaning and finial period, and it significantly increased the levels of fecal mucin-2 (MUC2), fecal short-chain fatty acids (SCFAs), and relative abundance of fecal Lactobacillus spp., and Bifidobacterium genus. Moreover, early intervention with FMT+C+S reduced the diarrhea rate during the experiment. FMT+C+S also decreased the level of plasma diamine oxidase (DAO) and D-lactate (D-LA), and relative abundance of fecal E. coli during the suckling period. In summary, early intervention with FMT combining CB and SB improved the growth performance, intestinal barrier function, fecal SCFAs concentration, and fecal Lactobacillus and Bifidobacterium of piglets.

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“…Several studies have reported the nutritional and health functions of C. butyricum in piglets, and the mechanisms by which the specific bacterial stain contributes to the regulation of the intestinal microecological balance, improves intestinal barrier function, modulates immune responses and regulates fat metabolism (Chen et al, ; Liao et al, ; Sato et al, ; Takahashi et al, ; Zhang, Chen, et al, ; Zhang, Sun, et al, ). We previously found that C. butyricum supplementation exerted beneficial effects in improving intestinal integrity of weaned piglets, and these protective effects of C. butyricum on the intestine could be closely related to the reduced expression of intestinal proinflammatory cytokines via inhibition of the ASC‐independent NLRP3 inflammasome signalling pathway (Li, Li, Zhu, Qiao, & Wang, ).…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we investigated the liver transcriptome profile of piglets administered with C. butyricum (the same strain used in our previous experiment) (Li et al, ) to identify new molecular mechanisms which enhance health and growth performance in weaned animals.…”

Section: Introductionmentioning

confidence: 99%

Dietary Clostridium butyricum supplementation modifies significantly the liver transcriptomic profile in weaned piglets

Qiao

2020

Animal Physiology Nutrition

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The addition of probiotics in swine nutrition is known to positively influence both health and growth. The current study investigates differences in the hepatic transcriptome profiles between weaned piglets supplemented with Clostridium butyricum (C. butyricum) and control animals that received no probiotic. The liver is an important metabolic organ that plays a critical role in oxidizing triglycerides for energy production, lipid synthesis and degradation, as well as immune regulation in animals. RNA‐Seq analysis was carried out on total RNA harvested from the liver of piglets fed with (n = 3) or without (n = 3) 5 × 105 C. butyricum CFU/g. Compared to the control piglets, 588 of the genes examined (352 up‐regulated and 236 down‐regulated) were significantly differentially expressed at a fold change > 2 and p < .05 in animals fed with C. butyricum. Quantitative real‐time reverse transcription PCR (qRT‐PCR) analysis was further used to validate the microarray expression results for 28 genes tested. The functional annotation analyses revealed several genes, processes and pathways with putative involvement in piglet growth and performance. Feeding swine with 5 × 105 C. butyricum CFU/g appears to reinforce their immune status as well as foster the cell cycle and improve the metabolism of carbohydrates, lipids and amino acids. This study provides valuable information about the expression profiles of mRNAs in piglet liver and in‐depth functional investigations of these mRNAs that could provide new insights into the molecular networks of growth, immune responses and nutrient metabolism in the porcine liver.

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Dietary supplementation with Clostridium butyricum helps to improve the intestinal barrier function of weaned piglets challenged with enterotoxigenic Escherichia coli K88

Abstract: The novelty of this study lies in the beneficial effects of C. butyricum on intestinal health, likely by improving intestinal barrier function and alleviating inflammatory reactions.

Cited by 51 publications

References 34 publications

Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances

Gut microbiota of newborn piglets with intrauterine growth restriction have lower diversity and different taxonomic abundances

Early Intervention Using Fecal Microbiota Transplantation Combined with Probiotics Influence the Growth Performance, Diarrhea, and Intestinal Barrier Function of Piglets

Dietary Clostridium butyricum supplementation modifies significantly the liver transcriptomic profile in weaned piglets

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