Dietary supplementation with casein/cyanidin-3-O-glucoside nanoparticles alters the gut microbiota in high-fat fed C57BL/6 mice

Lyu, Qiyan; Deng, Hongting; Wang, Shunxin; El‐Seedi, Hesham R.; Cao, Hui; Chen, Lei; Teng, Hui

doi:10.1016/j.foodchem.2023.135494

Cited by 18 publications

(11 citation statements)

References 38 publications

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“…Elevated levels of TC, TG, and LDL-C, along with low or high levels of HDL-C can contribute to obesity and increase the risk of developing other chronic illnesses, and high levels of TC and TG are often seen in individuals with obesity [ 23 , 24 ]. The effects of different dosages of LH on blood lipids among groups are shown in Figure 3 A–D.…”

Section: Resultsmentioning

confidence: 99%

“…The H&E-stained sections ( Figure 3 E) showed that the liver tissue structure of the NC group was intact, with hepatocytes organized in a neat cord-like pattern and no signs of steatosis. In the M group, liver cells showed small fat cavities and large fat droplets, indicating the presence of excessive hepatic fat, which is a characteristic feature of non-alcoholic fatty liver disease (NAFLD) [ 23 ]. Additionally, the size of the lipid droplets noticeably decreased following LH treatment, leading to a visible improvement in liver tissue damage compared to the M group.…”

Section: Resultsmentioning

confidence: 99%

“…An increase in the gut microbiota abundance and diversity often predicts improvement of physical function [ 23 ]. Hence, Ace and Chao 1 indices (species richness) and the Shannon index (species diversity) were used to evaluate α-diversity among the samples.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, HFD reduced the abundance of Oscillibacter , but LH reshaped it ( Figure 8 B). Studies have indicated that Oscillibacter has a negative correlation with weight, epididymal fat index, and levels of TC and HDL-C [ 23 ]. In our study, this particular species showed a strong negative correlation with body weight, weight gain, and LDL-C levels.…”

Section: Resultsmentioning

confidence: 99%

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Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Zhu,

Hou

2024

Foods

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Lactarius hatsudake (LH), a great wild endemic fungus, contains rich nutritional components with medicinal properties. The effects of LH on body weight, liver weight, liver injury, blood lipids, and gut microbiota in C57BL/6 mice fed a high-fat diet (HFD) for 8 weeks was examined in this research. Though there was no clear impact on weight loss, the findings indicate that LH treatment effectively decreased liver damage caused by HFD, as well as lowered serum total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels. Additionally, it positively influenced gut microbiota to resemble that of mice on a normal diet. In HFD-fed mice, LH markedly boosted the levels of Parabacteroides, unclassified Muribaculaceae, Oscillibacter, and unclassified Oscillospiraceae, while reducing the abundance of Lachnospiraceae NK4A136 group and Erysipelatoclostridium, as well as the ratio of Firmicutes to Bacteroidetes. Further analysis of correlation indicate a possible connection between obesity and gut microbiota. Obesity-related indices show a positive association with unclassified Eubacterium coprostanoligenes group, Blautia, and Erysipelatoclostridium, while displaying a negative correlation with unclassified Muribaculaceae, unclassified Clostridia vadinBB60 group, Helicobacter, Oscillibacter, unclassified Ruminococcaceae, Parabacteroides, and unclassified Oscillospiraceae. The results suggest that LH can help combat obesity and may have the potential to be utilized as a functional food.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Zhu,

Hou

2024

Foods

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“…The results of H&E staining and Oil Red O staining clearly demonstrated that C3G effectively reduced hepatic lipid accumulation. This illustrated that C3G intervention had a modulatory effect on lipid metabolism, Lyu et al also discovered casein-C3G could prevent the accumulation of liver lipid in HFD-fed mice [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Cyanidin-3-O-Glucoside Alleviates Alcoholic Liver Injury via Modulating Gut Microbiota and Metabolites in Mice

Zhu,

Cao,

et al. 2024

Nutrients

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Alcoholic liver disease (ALD) is primarily caused by long-term excessive alcohol consumption. Cyanidin-3-O-glucoside (C3G) is a widely occurring natural anthocyanin with multiple biological activities. This study aims to investigate the effects of C3G isolated from black rice on ALD and explore the potential mechanism. C57BL/6J mice (male) were fed with standard diet (CON) and Lieber-DeCarli liquid-fed (Eth) or supplemented with a 100 mg/kg/d C3G Diet (Eth-C3G), respectively. Our results showed that C3G could effectively ameliorate the pathological structure and liver function, and also inhibited the accumulation of liver lipids. C3G supplementation could partially alleviate the injury of intestinal barrier in the alcohol-induced mice. C3G supplementation could increase the abundance of Norank_f_Muribaculaceae, meanwhile, the abundances of Bacteroides, Blautia, Collinsella, Escherichia-Shigella, Enterococcus, Prevotella, [Ruminococcus]_gnavus_group, Methylobacterium-Methylorubrum, Romboutsia, Streptococcus, Bilophila, were decreased. Spearman’s correlation analysis showed that 12 distinct genera were correlated with blood lipid levels. Non-targeted metabolic analyses of cecal contents showed that C3G supplementation could affect the composition of intestinal metabolites, particularly bile acids. In conclusion, C3G can attenuate alcohol-induced liver injury by modulating the gut microbiota and metabolites, suggesting its potential as a functional food ingredient against alcoholic liver disease.

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Cyanidin‐3‐O‐glucoside ameliorates hydrogen peroxide‐induced oxidative stress by regulating HMGCR‐mediated cholesterol anabolism in HEK‐293T cells

Liu,

Zhang,

Dai

et al. 2024

Food Science & Nutrition

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Cyanidin‐3‐O‐glucoside (C3G), as a typical anthocyanin, exhibits excellent antioxidant effects. This study aimed to demonstrate the role and mechanism of C3G in regulating 3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase (HMGCR)‐mediated cholesterol anabolism on H2O2‐induced oxidative stress in HEK‐293T cells. Firstly, the inhibitory effect of C3G on oxidative stress was confirmed by CCK‐8, ROS, and mitochondrial membrane potential (MMP) experiments. Then, proteomics was used to investigate and screen differentially expressed proteins in inhibiting cellular oxidative stress by C3G. HMGCR was screened as a key differentially expressed protein by proteomic analysis. The results verified that C3G could reduce cholesterol levels by inhibiting sterol regulatory element‐binding protein (SREBP2)/HMGCR pathway, increasing ATP, and reducing acetyl‐CoA. Finally, HMGCR had been shown to positively increase ROS accumulation and decrease MMP, which were reversed by intervention of C3G through a series of knockdown and overexpression experiments. In conclusion, the results demonstrated that C3G could inhibit the disorder of cholesterol synthesis in oxidative stress cells by regulating the ROS/SREBP2/HMGCR pathway.

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Dietary supplementation with casein/cyanidin-3-O-glucoside nanoparticles alters the gut microbiota in high-fat fed C57BL/6 mice

Cited by 18 publications

References 38 publications

Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

Cyanidin-3-O-Glucoside Alleviates Alcoholic Liver Injury via Modulating Gut Microbiota and Metabolites in Mice

Cyanidin‐3‐O‐glucoside ameliorates hydrogen peroxide‐induced oxidative stress by regulating HMGCR‐mediated cholesterol anabolism in HEK‐293T cells

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