Dietary supplementation with biogenic selenium nanoparticles alleviate oxidative stress-induced intestinal barrier dysfunction

Abstract

Selenium (Se) is an essential micronutrient that promotes body health. Endemic Se deficiency is a major nutritional challenge worldwide. The low toxicity, high bioavailability, and unique properties of biogenic Se nanoparticles (SeNPs) allow them to be used as a therapeutic drug and Se nutritional supplement. This study was conducted to investigate the regulatory effects of dietary SeNPs supplementation on the oxidative stress-induced intestinal barrier dysfunction and its association with mitochondrial function and gut microbiota in mice. The effects of dietary SeNPs on intestinal barrier function and antioxidant capacity and its correlation with gut microbiota were further evaluated by a fecal microbiota transplantation experiment. The results showed that Se deficiency caused a redox imbalance, increased the levels of pro-inflammatory cytokines, altered the composition of the gut microbiota, and impaired mitochondrial structure and function, and intestinal barrier injury. Exogenous supplementation with biogenic SeNPs effectively alleviated diquat-induced intestinal barrier dysfunction by enhancing the antioxidant capacity, inhibiting the overproduction of reactive oxygen species (ROS), preventing the impairment of mitochondrial structure and function, regulating the immune response, maintaining intestinal microbiota homeostasis by regulating nuclear factor (erythroid-derived-2)-like 2 (Nrf2)-mediated NLR family pyrin domain containing 3 (NLRP3) signaling pathway. In addition, Se deficiency resulted in a gut microbiota phenotype that is more susceptible to diquat-induced intestinal barrier dysfunction. Supranutritional SeNPs intake can optimize the gut microbiota to protect against intestinal dysfunctions. This study demonstrates that dietary supplementation of SeNPs can prevent oxidative stress-induced intestinal barrier dysfunction through its regulation of mitochondria and gut microbiota.

Biogenic Selenium Nanoparticles Alleviate Intestinal Epithelial Barrier Damage through Regulating Endoplasmic Reticulum Stress-Mediated Mitophagy

“…Likewise, selenium enhances mitochondrial respiration, increases mitochondrial content, and up-regulates mediators of mitochondrial biogenesis in placental cells and tissues [ 27 ]. Biogenic SeNPs can alleviate oxidative stress-induced mitochondrial dysfunction [ 54 ]. In this study, the autophagy of intestinal epithelial cells is significantly enhanced after oxidative stress and TM-induced ERS, as evidenced by the fact that the p-AMPK/AMPK, p-mTOR, LC3B-II/LC3B-I ratio, Beclin1, ULK1, PINK1, and Parkin are significantly increased, and that the expression of SQSTM1/p62 is decreased remarkably, accompanied by the occurrence of autophagosomes in intestinal epithelial cells.…”

Feeding foliar nano-selenium biofortified panax notoginseng could reduce the occurrence of glycolipid metabolism disorder in mice caused by high-fat diets

“…At present, studies showed that the increase in the abundance of these microorganisms is negatively correlated with the risk of disease induced by HFD ( 49 – 52 ). Interestingly, some studies showed that dietary selenium supplementation can improve their relative abundance ( 53 , 54 ). Therefore, the increase of PNS and selenium content jointly regulate the gut microbiota and reduce the negative effects of HFD.…”

Selenium Nanoparticles-Enriched Lactobacillus casei ATCC 393 Prevents Cognitive Dysfunction in Mice Through Modulating Microbiota-Gut-Brain Axis

“…or toluidine blue staining according to standard procedures. 31 Images of the slices were obtained using inverted microscope (Leica DMIL, Germany).…”

“…4 h prior to the end of the experiment, mice were given 400 mg/kg B.W. of FITC-dextran by gavage, 31 and the concentration of FITC-dextran in serum was measured with a Multi-Mode Microplate Reader (Bio-Rad, Hercules, USA).…”

“…27 Our previous study demonstrated that SeNPs synthesized by Lactobacillus casei ATCC 393 (L. casei ATCC 393) possess significant antioxidant and anti-inflammatory activities in vitro and in vivo and dietary supplementation with SeNPs can prevent oxidative stress-induced intestinal barrier dysfunction through its regulation of gut microbiota. [29][30][31] Moreover, our latest research found that biogenic SeNPs synthesized by L. casei ATCC 393 can effectively alleviate the Aβ 25-35 -induced apoptosis of PC12 cells. Therefore, this study was conducted to investigate the preventive effects of SeNPs-enriched L. casei ATCC 393 (L. casei ATCC 393-SeNPs) on the AD mice model induced by D-galactose (D-gal)/aluminum chloride (AlCl 3 ) and its relationship with microbiota-gut-brain axis.…”