Dietary supplementation with arginine and glutamic acid alters the expression of amino acid transporters in skeletal muscle of growing pigs

Hu, Chengjun; Li, F N; Duan, Yehui; Zhang, T; Li, H W; Yin, Yulong; Wu, Guoyao; Kong, Xiangfeng

doi:10.1007/s00726-019-02748-9

Cited by 14 publications

(12 citation statements)

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“…However, no changes were observed in serum metabolites of the pigs fed with betaine or glycine. The latest study indicates that specific amino acids could enhance protein synthesis and promote skeletal muscle growth through activating the key signaling pathway ( Cholewa et al., 2014 ; Hu et al., 2019 ). In the present experiment, betaine or glycine partly changed the free amino acid profiles in serum and LD muscle.…”

Section: Discussionmentioning

confidence: 99%

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Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

et al. 2021

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The objective of the study is to evaluate and compare the effects of betaine or glycine on carcass trait, meat quality and lipid metabolism of finishing Huan Jiang mini-pigs. Betaine called trimethylglycine is a methyl derivative of glycine, but few researches were conducted to compare the impact of dietary betaine and glycine on pigs. One hundred and forty-four Huan Jiang mini-pigs (body weight = 10.55 ± 0.15 kg; 70 d) were randomly divided to 3 treatment groups (basal diet, glycine or betaine). Results indicated that dietary betaine increased the average daily gain (ADG) and final weight ( P < 0.05). Dietary glycine or betaine markedly reduced average backfat thickness ( P < 0.05) and heightened lean percentage ( P < 0.01) compared to the control group. Moreover, in comparison with the control group, betaine significantly improved the redness (a∗) and tenderness (shear force) of the longissimus dorsi (LD) muscle ( P < 0.05), whereas glycine only raised the value of a∗ of the LD muscle ( P < 0.05). These results showed that diet supplemented with 0.25% betaine and equimolar amounts of glycine could regulate cascass trait and meat quality of finishing Huan Jiang mini-pigs, and the effect of betaine was superior to that of glycine.

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Section: Discussionmentioning

confidence: 99%

“…The free amino acid profile in serum was measured as previously described ( Hu et al., 2019 ). Firstly, 1 mL of 8% 5-sulfosalicylic acid was added to tube including 1 mL of serum, mixed, stood for 15 min, and then centrifuged at 9,390 × g at room temperature for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

et al. 2021

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“…The free amino acid concentration in the LD and PM muscles was determined as described previously 17 …”

Section: Methodsmentioning

confidence: 99%

“…The free amino acid concentration in the LD and PM muscles was determined as described previously. 17 Muscle fiber characteristics Muscle tissues fixed in 40 g/kg paraformaldehyde were paraffinembedded and sectioned at 5 μm thickness, followed by staining with hematoxylin-eosin (HE). Five fascicles were randomly selected from each section and used for image analysis using a light microscope (Olympus CX41, Tokyo, Japan).…”

Section: Determination Of Muscle Free Amino Acid Concentrationmentioning

confidence: 99%

Effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in both low and normal birth weight piglets

Peng

Yang

et al. 2020

J Sci Food Agric

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BACKGROUND: Maternal nutrition during gestation plays a vital role in fetal development. The effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in low and normal birth weight offspring were investigated by using 80 gilts randomly allotted to T1 and T2 groups and treated respectively with a gradual-increase (T1) and a convex transition (T2) feeding strategy, with no difference in total feed intake. RESULTS: T2 group was seen to have a higher percentage of piglets with birth weight less than 500 g, while T1 group was shown to have a higher percentage of piglets with birth weight over 700 g. Meanwhile, for both low and normal birth weight piglets, T1 group was higher than T2 group in terms of muscle free amino acid concentration, mRNA expression levels of muscle growthrelated factors, relative muscle fiber number and cross-sectional area. We must emphasize that the T2 group was shown to improve glucose tolerance, slow-twitch muscle fiber protein levels, and muscle mitochondrial function only in low birth weight piglets. CONCLUSION: The convex transition feeding strategy can decrease the percentage of piglets with birth weight over 700 g, while improving glucose tolerance, slow-twitch muscle fiber protein levels, and muscle mitochondrial function in low birth weight piglets. Our findings provide new evidence for the potential importance of nutritional strategies during gestation, especially for improving the glucose tolerance and muscle development of low birth weight neonatal.

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“…ASCT2 is a neutral amino acid transporter and EAAC1 is an acidic amino acid transporter. [29][30][31][32][33] These transporters mainly function in the absorption of amino acids. Based on the qRT-PCR results, there were decreases in the messenger RNA (mRNA) levels of the amino acid transporters y + LAT1, SLC7A1, ASCT2, and EAAC1 in IPEC-J2 cells triggered by FB 1 exposure in a roughly dosage-dependent manner (Figure 3A-D).…”

Section: Fb 1 Altered the Transcriptional Levels Of Key Nutrient Trmentioning

confidence: 99%

Effect of fumonisin B₁on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells

Chen

Zhou

Yuan

et al. 2021

J Biochem & Molecular Tox

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Fumonisin B1 (FB1) is a common environmental mycotoxin produced by molds such as Fusarium verticillioides. The toxin poses health risks to domestic animals, including pigs, through FB1‐contaminanted feed. However, the cytotoxicity of FB1 to porcine intestines has not been fully analyzed. In the present study, the effects of FB1 on oxidative stress and nutrient transporter‐associated genes of the porcine intestinal IPEC‐J2 cells were explored. FB1 decreased IPEC‐J2 proliferation but did not trigger reactive oxygen species (ROS) overproduction. Meanwhile, FB1 reduced the expression levels of the transporters l‐type amino acid transporter‐1 (y+LAT1), solute carrier family 7 member 1 (SLC7A1), solute carrier family 1 member 5 (ASCT2), and excitatory amino acid carrier 1 (EAAC1); in addition, FB1 reduced the levels of the fatty acid transporters long‐chain fatty acid transport protein 1 (FATP1) and long‐chain fatty acid transport protein 4 (FATP4) as well as glucose transporters Na+/glucose cotransporter 1 (SGLT1) and glucose transporter 2 (GLUT2). FB1 stimulation increased the expression levels of peptide transporter peptide transporter 1 (PepT1) and metal ion transport‐related gene zinc transporter 1 (ZNT1). Moreover, metal ion transporter divalent metal transporter 1 (DMT1) expression was depressed by a higher dosage of FB1. The data indicate that FB1 results in aberrant expression of nutrient transporters in IPEC‐J2 cells, thereby exerting its toxicity even though it fails to exert ROS‐dependent oxidative stress.

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Dietary supplementation with arginine and glutamic acid alters the expression of amino acid transporters in skeletal muscle of growing pigs

Cited by 14 publications

References 52 publications

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

Effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in both low and normal birth weight piglets

Effect of fumonisin B₁on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells

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Dietary supplementation with arginine and glutamic acid alters the expression of amino acid transporters in skeletal muscle of growing pigs

Cited by 14 publications

References 52 publications

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

Dietary supplementation with betaine or glycine improves the carcass trait, meat quality and lipid metabolism of finishing mini-pigs

Effects of different maternal feeding strategies from day 1 to day 85 of gestation on glucose tolerance and muscle development in both low and normal birth weight piglets

Effect of fumonisin B1on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells

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Effect of fumonisin B₁on oxidative stress and gene expression alteration of nutrient transporters in porcine intestinal cells