Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice

Alvarado, Carmen; Álvarez, Pedro; Puerto, Marta; Gausserès, Nicolas; Jiménez, Liliana; Fuente, Mónica De la

doi:10.1016/j.nut.2006.05.007

Cited by 91 publications

(67 citation statements)

References 51 publications

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“…In the present study, -tocopherol at a concentration of 0.1 mg/ml did not cause an increase in the chemotactic activity of leukocytes, as it did at concentrations of 0.2 mg/ml and 0.5 mg/ml. Contrasting results were obtained in research on mice by Alvarado et al (2006) who observed a significant increase in the chemotactic activity of macrophages after administration of small amounts of -tocopherol or ascorbic acid. There have also been changes observed in human subjects given both antioxidants, including changes in the production of inflammatory cytokines IL-1F2 and TNF-, which was found to be significantly greater than in the case of cells isolated from subjects who received only -tocopherol or ascorbic acid.…”

Section: Discussioncontrasting

confidence: 65%

“…Antioxidant administration may inhibit the inflammatory process by decreasing expression of cell adhesion molecules, reduce pro-inflammatory activity of neutrophils, i.e. inhibit pro-inflammatory cytokine production, but may enhance the anti-microbial activity of the leukocytes (Alvarado et al 2006;EkstrandHammarström et al 2006). In addition, increased supplementation of -tocopherol in vitro enhanced the chemotactic index but had no effect on antibody-dependent cellular cytotoxicity (Eicher et al 1994).…”

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confidence: 99%

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In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

et al. 2009

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The aim of the present study was to evaluate the effect of two different antioxidants, -tocopherol and ascorbic acid, on the chemotactic activity and susceptibility to Mannheimia haemolytica leukotoxin (Lkt) of white blood cells obtained from calves exposed to transport in vitro.Both antioxidants used were found to affect the chemotactic activity of white blood cells. Supplementation of -tocopherol at a concentration of 0.2 mg/ml and 0.5 mg/ml induced significant (P  0.05) increase of chemotactic activity in white blood cells compared to control. In case of ascorbic acid supplementation the highest chemotactic activity was observed at a concentration of 0.5 mg/ml. The results obtained in microtitration (MTT) assay also suggest that high concentrations of -tocopherol (0.5 mg/ml) play a role in protecting bovine leukocytes against the cytotoxic effect of Lkt, the susceptibility of the leukocytes on M. haemolytica Lkt was lower in comparison to control about 6%.The antioxidants used in this study have been found to have a protective effect on cells participating in the immune response to M. haemolytica. This particularly applies to high concentrations of -tocopherol. The clinical aspect of these results may lie in protection of leukocytes exposed to leukotoxin of M. haemolytica.It should be emphasized that the results provide only partial information on the effect of the antioxidants studied on cells participating in an organism's defence mechanisms. For an evaluation that would enable proper prevention of bovine respiratory diseases, research should be continued and expanded to include analysis of cellular structures.

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Section: Discussioncontrasting

confidence: 65%

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confidence: 99%

In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

et al. 2009

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“…Since FR were accused as causal factors in a large number of diseases, these were sometimes referred as ''Free Radical Diseases''. Some of the important diseases and health issues in this category are cancer [61][62][63][64][65][66][67], cardiovascular diseases [68][69][70][71][72][73], atherosclerosis [74][75][76][77][78][79][80][81], neurological disorders [82][83][84][85][86], renal disorders [87][88][89][90], liver disorders [91][92][93], hypertension [50,94,95], rheumatoid arthritis [96,97], adult respiratory distress syndrome, auto-immune deficiency diseases [98,99], inflammation, degenerative disorders associated with aging [100][101][102][103], diabetes mellitus [104][105]…”

Section: Reactive Oxygen Species and Reactive Nitrogen Species Transimentioning

confidence: 99%

Reconvene and Reconnect the Antioxidant Hypothesis in Human Health and Disease

Singh

Chandra

Mahdi

et al. 2010

Indian J Clin Biochem

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The antioxidants are essential molecules in human system but are not miracle molecules. They are neither performance enhancers nor can prevent or cure diseases when taken in excess. Their supplemental value is debateable. In fact, many high quality clinical trials on antioxidant supplement have shown no effect or adverse outcomes ranging from morbidity to all cause mortality. Several Chochrane Meta-analysis and Markov Model techniques, which are presently best available statistical models to derive conclusive answers for comparing large number of trials, support these claims. Nevertheless none of these statistical techniques are flawless. Hence, more efforts are needed to develop perfect statistical model to analyze the pooled data and further double blind, placebo controlled interventional clinical trials, which are gold standard, should be implicitly conducted to get explicit answers. Superoxide dismutase (SOD), glutathione peroxidase and catalase are termed as primary antioxidants as these scavenge superoxide anion and hydrogen peroxide. All these three enzymes are inducible enzymes, thereby inherently meaning that body increases or decreases their activity as per requirement. Hence there is no need to attempt to manipulate their activity nor have such efforts been clinically useful. SOD administration has been tried in some conditions especially in cancer and myocardial infarction but has largely failed, probably because SOD is a large molecule and can not cross cell membrane. The dietary antioxidants, including nutrient antioxidants are chain breaking antioxidants and in tandem with enzyme antioxidants temper the reactive oxygen species (ROS) and reactive nitrogen species (RNS) within physiological limits. Since body is able to regulate its own requirements of enzyme antioxidants, the diet must provide adequate quantity of non-enzymic antioxidants to meet the normal requirements and provide protection in exigent condition. So far, there is no evidence that human tissues ever experience the torrent of reactive species and that in chronic conditions with mildly enhanced generation of reactive species, the body can meet them squarely if antioxidants defense system in tissues is biochemically optimized. We are not yet certain about optimal levels of antioxidants in tissues. Two ways have been used to assess them: first by dietary intake and second by measuring plasma levels. Lately determination of plasma/serum level of antioxidants is considered better index for diagnostic and prognostic purposes. The recommended levels for vitamin A, E and C and beta carotene are 2.2-2.8 lmol/l; 27.5-30 lmol/l; 40-50 lmol/l and 0.4-0.5 lmol/l, respectively. The requirement and recommended blood levels of other dietary antioxidants are not established. The resolved issues are (1) essential to scavenge excess of radical species (2) participants in redox homeostasis (3) selective antioxidants activity against radical species (4) there is no universal antioxidant and 5) therapeutic value in case of deficiency. The overar...

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“…The importance of dietary antioxidant components in the prevention of chronic disease and improvement of health quality has attracted huge research attention in the past few decades (Alvarado et al 2006;Skotti et al 2014). *Corresponding authors.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis, antioxidant and antilipid peroxidation effects of a medicinal plant, Adhatoda vasica

Bajpai

Agrawal

Bang

et al. 2015

Frontiers in Life Science

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Many diseases are associated with oxidative stress caused by reactive oxygen species and free radicals generated in living cells. This study deals with finding naturally occurring antioxidants of plant origin. The study investigated the phytochemistry, antioxidant and free radical scavenging potential of ethanolic leaf extract of Adhatoda vasica (ELEAV) using different antioxidant models. The phytochemical analysis of ELEAV revealed the presence of alkaloids, flavonoids, terpenoids, saponins, phenols and steroids. ELEAV also showed an antioxidant capacity, with inhibition of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by 69.23%. ELEAV had potent inhibitory effects on scavenging nitric oxide, superoxide and hydroxyl radicals, by 65.24%, 61.54% and 61.24%, respectively. Moreover, ELEAV displayed a concentration-dependent reducing power activity and potent inhibitory effects on ferric ion-induced lipid peroxidation (68.26%) in bovine brain extract. These findings confirm the biological efficacy of A. vasica as a potential source of natural antioxidants.

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Dietary supplementation with antioxidants improves functions and decreases oxidative stress of leukocytes from prematurely aging mice

Cited by 91 publications

References 51 publications

In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

Reconvene and Reconnect the Antioxidant Hypothesis in Human Health and Disease

Phytochemical analysis, antioxidant and antilipid peroxidation effects of a medicinal plant, Adhatoda vasica

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