Dietary Supplementation of Selenoneine-Containing Tuna Dark Muscle Extract Effectively Reduces Pathology of Experimental Colorectal Cancers in Mice

Masuda, Junko; Umemura, Chiho; Yokozawa, Miki; Yamauchi, Ken; Seko, Takuya; Yamashita, Michiaki; Yamashita, Yumiko

doi:10.3390/nu10101380

Cited by 19 publications

(13 citation statements)

References 59 publications

(76 reference statements)

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“…Selenoneine is the selenium-analog of EGT and was discovered in 2010 in the blood of tuna fish [ 13 , 14 ]. Similar in function to EGT, selenoneine possesses powerful antioxidant properties in vitro and has also been shown to detoxify heavy metals such as mercury in vivo [ 14 , 54 , 55 ]. Humans obtain selenoneine by consuming fish and accumulating it in their cells via the cation/carnitine transporter OCTN1 [ 14 , 54 , 55 ].…”

Section: Resultsmentioning

confidence: 99%

“…Similar in function to EGT, selenoneine possesses powerful antioxidant properties in vitro and has also been shown to detoxify heavy metals such as mercury in vivo [ 14 , 54 , 55 ]. Humans obtain selenoneine by consuming fish and accumulating it in their cells via the cation/carnitine transporter OCTN1 [ 14 , 54 , 55 ]. It is interesting to note that selenoneine binds less tightly to Sec-TrxR, as reflected by a 5-fold higher K M value in comparison to ESSE but is only turned over 2-fold faster.…”

Section: Resultsmentioning

confidence: 99%

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Oxidized Forms of Ergothioneine Are Substrates for Mammalian Thioredoxin Reductase

et al. 2022

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Ergothioneine (EGT) is a sulfur-containing amino acid analog that is biosynthesized in fungi and bacteria, accumulated in plants, and ingested by humans where it is concentrated in tissues under oxidative stress. While the physiological function of EGT is not yet fully understood, EGT is a potent antioxidant in vitro. Here we report that oxidized forms of EGT, EGT-disulfide (ESSE) and 5-oxo-EGT, can be reduced by the selenoenzyme mammalian thioredoxin reductase (Sec-TrxR). ESSE and 5-oxo-EGT are formed upon reaction with biologically relevant reactive oxygen species. We found that glutathione reductase (GR) can reduce ESSE, but only with the aid of glutathione (GSH). The reduction of ESSE by TrxR was found to be selenium dependent, with non-selenium-containing TrxR enzymes having little or no ability to reduce ESSE. In comparing the reduction of ESSE by Sec-TrxR in the presence of thioredoxin to that of GR/GSH, we find that the glutathione system is 10-fold more efficient, but Sec-TrxR has the advantage of being able to reduce both ESSE and 5-oxo-EGT directly. This represents the first discovered direct enzymatic recycling system for oxidized forms of EGT. Based on our in vitro results, the thioredoxin system may be important for EGT redox biology and requires further in vivo investigation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Oxidized Forms of Ergothioneine Are Substrates for Mammalian Thioredoxin Reductase

et al. 2022

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“…Salmon-fed animals showed a slightly lower intestinal carcinogenesis when compared to a standard diet and to diets containing meat from terrestrial animals [57]. More recently, dietary supplementation with tuna muscle extract rich in selenoeine (an Se-protein with antioxidant activity present in the blood and muscle of tuna) was reported to decrease colon carcinogenesis in a colitis-associated model of CRC in mice [58].…”

Section: Fish and Fish-oil Intake And Colorectal Carcinogenesis In Animal Modelsmentioning

confidence: 99%

Fish Consumption and Colorectal Cancer Risk: Meta-Analysis of Prospective Epidemiological Studies and Review of Evidence from Animal Studies

Caini¹,

Chioccioli

Pastore³

et al. 2022

Cancers

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Background: Epidemiological studies on the association between fish consumption and colorectal cancer (CRC) risk have yielded inconsistent results, despite evidence from preclinical studies that long-chain ω-3 polyunsaturated fatty acids inhibit colorectal carcinogenesis. We conducted a meta-analysis of prospective epidemiological studies investigating the association between fish consumption and CRC risk among humans and reviewed studies examining the link between fish components and colorectal carcinogenesis in animal models. Methods: We included studies published until November 2020. We calculated the summary risk ratio (SRR) and 95% confidence intervals (CI) through random effects meta-analysis models in order to summarize evidence from studies among humans. Results: Twenty-five prospective epidemiological studies encompassing 25,777 CRC cases were included. Individuals in the highest (vs. lowest) category of fish consumption had a significantly reduced risk of CRC (SRR 0.94, 95%CI 0.89–0.99). In dose–response meta-analysis, a 50-g increment in the daily consumption of fish was associated with a statistically significant 4% reduction in CRC risk (SRR 0.96, 95%CI 0.92–0.99). Preclinical studies (n = 25) identified multiple mechanisms of action of fish and fish components on colorectal carcinogenesis. Conclusions: Dietary recommendations for cancer prevention should take into account the evidence from epidemiological and preclinical studies that increasing fish consumption may be effective in preventing CRC.

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“…Support for this idea comes from the demonstration that Schizosaccharomyces pombe grown in selenium‐enriched media produces small but detectable amounts of 2 in a sulfoxide synthase‐dependent manner . The limited accessibility of selenoneine by fermentation or by isolation from marine fish is a major roadblock for exploring and exploitation of selenoneine as a therapeutic . A strategy for chemical synthesis has been published recently …”

Section: Introductionmentioning

confidence: 99%

Selenocysteine as a Substrate, an Inhibitor and a Mechanistic Probe for Bacterial and Fungal Iron‐Dependent Sulfoxide Synthases

Goncharenko

Flückiger

Liao

et al. 2020

Chemistry A European J

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Sulfoxide synthases are non-hemei ron enzymes that participatei nt he biosynthesis of thiohistidines, such as ergothioneine ando vothiol A. The sulfoxide synthase EgtB from Chloracidobacterium thermophilum (CthEgtB) catalyzes oxidative coupling between the side chains of N-a-trimethyl histidine( TMH) and cysteine (Cys) in ar eaction that entails complete reduction of molecular oxygen,c arbon-sulfur (CÀ S) and sulfur-oxygen (SÀO) bond formationa sw ell as carbon-hydrogen (CÀH) bond cleavage. In this report, we show that CthEgtB and other bacterial sulfoxide synthases cannote fficiently accept selenocysteine (SeCys) as as ubstrate in place of cysteine. In contrast, the sulfoxide synthase from the filamentous fungus Chaetomium thermophilum (CthEgt1) catalyzes CÀSa nd CÀSe bond formation at almost equale fficiency.W ed iscuss evidence suggesting that this functional differenceb etween bacterial and fungal sulfoxide synthases emerges from different modes of oxygen activation.[a] K.

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Dietary Supplementation of Selenoneine-Containing Tuna Dark Muscle Extract Effectively Reduces Pathology of Experimental Colorectal Cancers in Mice

Cited by 19 publications

References 59 publications

Oxidized Forms of Ergothioneine Are Substrates for Mammalian Thioredoxin Reductase

Oxidized Forms of Ergothioneine Are Substrates for Mammalian Thioredoxin Reductase

Fish Consumption and Colorectal Cancer Risk: Meta-Analysis of Prospective Epidemiological Studies and Review of Evidence from Animal Studies

Selenocysteine as a Substrate, an Inhibitor and a Mechanistic Probe for Bacterial and Fungal Iron‐Dependent Sulfoxide Synthases

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