Dietary supplementation of papaya (Carica papaya l.) leaf affects abundance of Butyrivibrio fibrisolvens and modulates biohydrogenation of C18 polyunsaturated fatty acids in the rumen of goats

Abstract

Jahromi (2018) Dietary supplementation of papaya (Caricapapaya l.) leaf affects abundance of Butyrivibriofibrisolvens and modulates biohydrogenation of C18 polyunsaturated fatty acids in the rumen of goats,

Dihydropyridine Enhances the Antioxidant Capacities of Lactating Dairy Cows under Heat Stress Condition

“…The procedures for Real-time PCR were as follows: pre-denaturalization for 3 min at 95.0 °C, 40 cycles of denaturalization for 30 s at 95.0 °C, annealing for 30 s at 60.0 °C, and extension for 30 s at 72.0 °C, followed by extension for 7 min at 72.0 °C. The relative contents of the above-mentioned eight microbes in ruminal fluids were calculated using total bacteria as a reference, as previously described [ 18 ] or designed using NCBI primer-Blast ( ).…”

“…The PCR system included 12.5 µL SYBR ® Green Real-Time PCR MasterMix (St. Louis, MO, USA), 0.5 µL forward primer (10 mM), 0.5 µL reverse primer (10 mM), 1 µL DNA template, and 9.5 µL sterile deionized water. The procedures for Real-time PCR were as follows: pre-denaturalization for 3 min at 95.0 • C, 40 cycles of denaturalization for 30 s at 95.0 • C, annealing for 30 s at 60.0 • C, and extension for 30 s at 72.0 • C, followed by extension for 7 min at 72.0 • C. The relative contents of the above-mentioned eight microbes in ruminal fluids were calculated using total bacteria as a reference, as previously described [18] or designed using NCBI primer-Blast (www.ncbi.nlm.nih.gov/tools/primer-blast/).…”

Modifying the farmer field school method to support on-farm adaptation of complex rice systems

“…In Malang, greater challenges with limited water availability and calcareous porous soil weakened fish and resulted in high mortality. Farmers suggested to distribute papaya leaves on the rice field as feed, believing that it has antiseptic, anti-parasitic and antimicrobial effects (Valladão, 2015;Jafari et al, 2018). Thus, three papaya trees were grown on rice bunds.…”

Modulatory effects of dietary tannins on polyunsaturated fatty acid biohydrogenation in the rumen: A meta-analysis

“…This indicates that further verification of the strategic function of tannins as modulators of rumen lipid metabolism is required. The in vitro and in sacco studies conducted by Jayanegara et al [6], Jafari et al [9] and Jafari et al [10] showed the potential of various tropical forage species as a tannin source in modulating BH to increase the flow of C18:3 n-3 and C18:2 n-6 to the duodenum. Several studies have also shown that some tropical forage species can improve production performance by providing bypass protein for ruminants [11,12].…”