Dietary sodium butyrate supplementation attenuates intestinal inflammatory response and improves gut microbiota composition in largemouth bass (Micropterus salmoides) fed with a high soybean meal diet

Chen, Weijun; Chang, Kuo-Song; Chen, Jialong; Zhao, Xiaoyu; Gao, Shiyang

doi:10.1007/s10695-021-01004-w

Cited by 42 publications

(15 citation statements)

References 63 publications

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“…Chlorogenic acid can regulate inflammatory reactions by reducing mRNA expression levels of tnfα [ 25 ]. Dietary sodium butyrate can improve intestinal morphology by increasing the villus width and villus height [ 26 ]. Because AMP can promote growth, antioxidant activity, and disease resistance [ 8 , 9 , 11 , 12 , 13 , 14 , 15 ], it could be used as a feed additive in largemouth bass culture.…”

Section: Introductionmentioning

confidence: 99%

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

Chen

et al. 2022

Antioxidants

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Atractylodes macrocephala polysaccharide (AMP) can enhance antioxidant defense and anti-inflammation, as the tolerance levels of AMP in aquaculture is important for additive utilization. However, the tolerance dose of AMP is unknown. We assess the tolerance levels of AMP in juvenile largemouth bass (3.38 ± 0.11 g) by feeding them a 0, 400, 4000, or 8000 mg/kg AMP supplemented diet for 10 weeks. The 400 mg/kg AMP dose increased growth performance. The Nrf2/Keap1 signaling pathway was activated, as indicated by Keap1 and Nrf2 protein levels in the liver. Enhanced activity of antioxidant enzymes (SOD, GPx), together with increased mRNA levels of antioxidant genes (sod, gpx) and decreased accumulation of reactive oxygen species (ROS) and MDA, was found in the liver, implying the antioxidant effect of AMP. Nutrient absorption was enhanced by AMP, as reflected by the increased length of intestinal villi and microvilli. However, 4000 and 8000 mg/kg AMP induced oxidant stress, as indicated by increased plasma ALT and AST content and decreased mRNA levels of antioxidant genes (sod, gpx) in the liver and intestinal tissues. Inflammatory reactions were also induced by high doses of AMP, as reflected by enhanced levels of pro-inflammatory cytokines (tnfα, nfκb) in the liver, intestinal, and kidney tissues and inhibited levels of anti-inflammatory cytokines (tgfβ, iκb). Histological analysis reveals inflammatory cell infiltration and tissue damage. Thus, the safe tolerance margin of AMP supplement for largemouth bass was 400–4000 mg/kg.

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Section: Introductionmentioning

confidence: 99%

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

Chen

et al. 2022

Antioxidants

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“…Tumor necrosis factor α, a pro‐inflammatory cytokine, is a biomarker of inflammation in aquacultured animals (Chen et al 2021; Sakai et al 2021). In this study, P deficiency significantly upregulated the TNF‐α expression in the liver, indicating that hepatic inflammation had occurred.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary phosphorus deficiency on the growth performance, hepatic lipid metabolism, and antioxidant capacity of Yellow River Carp Cyprinus carpio haematopterus

et al. 2023

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Objective The study aimed to evaluate the effects of phosphorus (P) deficiency in diets on growth performance, hepatic lipid metabolism, and antioxidant capacity in Yellow River Carp Cyprinus carpio haematopterus. Methods In this study, 72 healthy experimental fish (initial weight = 12.0 ± 0.1 g [mean ± SE]) were randomly selected and distributed to two groups, with three replicates in each group. The groups were fed either a P‐sufficient diet or a P‐deficient diet for 8 weeks. Result The P‐deficient feed significantly decreased the specific growth rate, feed efficiency, and condition factor of Yellow River Carp. Fish that were fed the P‐deficient feed demonstrated higher contents of triglyceride, total cholesterol (T‐CHO), and low‐density lipoprotein cholesterol in the plasma and a higher T‐CHO content in the liver compared to the P‐sufficient diet group. In addition, the P‐deficient diet significantly reduced the catalase activity level, decreased the glutathione content, and increased the malondialdehyde content in the liver and in the plasma. Furthermore, P deficiency in the diet significantly downregulated the messenger RNA expression of nuclear erythroid 2‐related factor 2 and peroxisome proliferator‐activated receptor α, whereas it upregulated the messenger RNA expression of tumor necrosis factor α and fatty acid synthase in the liver. Conclusion Dietary P deficiency reduced fish growth performance, induced fat deposition and oxidative stress, and impaired liver health.

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“…Dietary supplementation of glutamine or dipeptide products had positive effects on the mucosal thickness and crypt depth of hybrid grouper ( 24 ), turbot ( Scophthalmus maximus ) ( 57 ) and grass carp ( 52 ). The improvement of intestinal structure and morphology may be due to the fact that TB and AGn are the main energy sources of intestinal epithelial cells ( 15 , 58 ), which can provide energy for intestinal epithelial cells and stimulate their proliferation and differentiation, especially when the intestinal tract is damaged ( 34 , 59 , 60 ). In addition, the present study showed that AGn was superior to TB in improving the intestinal morphology of largemouth bass, especially in the fore- and midgut, and 2% AGn was the most effective.…”

Section: Discussionmentioning

confidence: 99%

Effects of tributyrin and alanyl-glutamine dipeptide on intestinal health of largemouth bass (Micropterus salmoides) fed with high soybean meal diet

et al. 2023

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To investigate the effects of dietary tributyrin (TB) and alanyl-glutamine (AGn) on the intestinal health of largemouth bass (Micropterus salmoides) fed with high-level soybean meal (SM) diet, six isonitrogenous (41.36%) and isolipidic (10.25%) diets were formulated and fed to largemouth bass (initial body weight 25.5 ± 0.5g) for 8 weeks. The two control diets contained 34.8% peanut meal (PM) and 41.3% SM, while the other four experimental diets supplemented TB at 0.1% (TB0.1), 0.2% (TB0.2) and AGn at 1% (AGn1), 2% (AGn2) in SM, respectively. The results showed that there were no significant differences in weight gain, survival rate, and hepatosomatic index among all groups (P>0.05), while feed coefficient rate in AGn1, AGn2 and TB0.2 groups was significantly lower than that in SM group (P< 0.05). Compared with the PM group, the intestinal inflammation of largemouth bass in SM group were obvious, accompanied by the damage of intestinal structure, the decrease of digestive enzyme activity, and the up-regulation of proinflammatory cytokines. Compared with the SM group, the activities of intestinal trypsin, lipase and foregut amylase in TB and AGn groups increased significantly (P<0.05), and the gene expression levels of acetyl-CoA carboxylase (ACC), caspase-3, caspase-8, caspase-9, tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β) were down-regulated, while the gene expression levels of target of rapamycin (TOR) and eIF4E-binding protein (4E-BP) were up-regulated in all experimental groups (P<0.05). It can be concluded that supplementation of 1%-2% AGn and 0.1%-0.2% TB can alleviate enteritis caused by high-level soybean meal, and the recommend level is 2% AGn and 0.2% TB.

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Dietary sodium butyrate supplementation attenuates intestinal inflammatory response and improves gut microbiota composition in largemouth bass (Micropterus salmoides) fed with a high soybean meal diet

Cited by 42 publications

References 63 publications

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Effects of dietary phosphorus deficiency on the growth performance, hepatic lipid metabolism, and antioxidant capacity of Yellow River Carp Cyprinus carpio haematopterus

Effects of tributyrin and alanyl-glutamine dipeptide on intestinal health of largemouth bass (Micropterus salmoides) fed with high soybean meal diet

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