Dietary selenium supplementation alleviates low salinity stress in the Pacific white shrimp Litopenaeus vannamei: growth, antioxidative capacity and hepatopancreas transcriptomic responses

Abstract

Selenium (Se) is an essential trace element associated with animal growth and antioxidant and metabolic processes. However, whether Se, especially organic Se with higher bioavailability, can alleviate the adverse effects of low salinity stress on marine economic crustacean species has not been investigated. Accordingly, juvenile Pacific white shrimp (Litopenaeus vannamei) were reared in two culture conditions (low and standard salinity) fed diets supplemented with increasing levels of L-selenomethionine (0.41, 0.84 and 1.14 mg/kg Se) for 56 days, resulting in four treatments: 0.41 mg/kg under standard seawater (salinity 31) and 0.41, 0.84 and 1.14 mg/kg Se under low salinity (salinity 3). The diet containing 0.84 mg/kg Se significantly improved the survival and weight gain of shrimp under low salinity stress and enhanced the antioxidant capacity of the hepatopancreas. The increased numbers of B and R cells may be a passive change in hepatopanclear histology in the 1.14 mg/kg Se group. Transcriptomic analysis found that L-selenomethionine was involved in the regulatory pathways of energy metabolism, retinol metabolism and steroid hormones. In conclusion, dietary supplementation with 0.84 mg/kg Se (twice the recommended level) effectively alleviated the effects of low salinity stress on L. vannamei by regulating antioxidant capacity, hormone regulation and energy metabolism.

Selenomethionine Attenuated H2O2-Induced Oxidative Stress and Apoptosis by Nrf2 in Chicken Liver Cells

“…Additionally, Gallegos et al reported that incubation with 345.86 ng/mL SS markedly increased the mRNA stability of thioredoxin reductase, a homodimeric Sec-containing protein, in HT-29 colon cancer cells [34]. Consistent with previous studies, our results indicated that both SS and SM could improve the mRNA stabil- As Se serves as an important component of GPx [30], its activity was also detected. As shown in Figure 1B,C, both 100 ng/mL SS and SM significantly enhanced GPx activity (p < 0.01).…”

“…As Se serves as an important component of GPx [ 30 ], its activity was also detected. As shown in Figure 1 B,C, both 100 ng/mL SS and SM significantly enhanced GPx activity ( p < 0.01).…”