Dietary resveratrol alters lipid metabolism-related gene expression of mice on an atherogenic diet

Ahn, Jiyun; Cho, Il-Jin; Kim, Suna; Kwon, Dae-Young; Ha, Tae‐Youl

doi:10.1016/j.jhep.2008.08.012

Cited by 161 publications

(104 citation statements)

References 42 publications

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“…Moderate exercise increases energy expenditure and has been recommended for the treatment of obesity [21]. Also, it is known that resveratrol treatments reduce body weight and liver weight compared to non-treatment in animals [22]. Thus, it was meaningful to compare the effectiveness of exercise and resveratrol on obesity-induced metabolic complications.…”

Section: Discussionmentioning

confidence: 99%

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

Jeong

Lee

Park

et al. 2015

J. Exerc. Nutr, Biochem.

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The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice. JENB., Vol. 19, No. 2, pp.65-72, 2015 [Purpose] The aim of this study was to compare the effectiveness of either resveratrol supplementation or exercise training on macrophage infiltration and switching from M1 to M2 kupffer cells in high fat diet mice.[Methods] C57BL/6 mice were separated into 5 groups: normal diet (ND; n = 6), high-fat diet (HD; n = 6), high-fat diet with resveratrol (HR; n = 6), high-fat diet with exercise (HE; n = 6) or high-fat diet with resveratrol and exercise (HRE; n = 6). Resveratrol supplementation mice were orally gavaged with resveratrol (25mg/kg of body weight) dissolved in 50% propylene glycol. Exercise mice ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/week for 12 weeks.[Results] After 12 weeks of intervention, the liver was analyzed. F4/80 expression was evaluated by western blot while CD11c and CD163 mRNA expressions were evaluated by RT-PCR. The weights of the body and liver were significantly increased in the HD and HR group compared to the ND group (p < 0.01). However, the weights were most effectively reduced in the HE and HRE groups compared to the HD group (p < 0.05). The macrophage marker, F4/80 expression was significantly lower in the HE and HRE groups compared to the HD group (p < 0.05). mRNA expression of the M1 macrophage marker, CD11c, in the HD group was significantly increased compared to the ND group (p < 0.01). mRNA expression of the M2 macrophage specific marker, CD163, in the HE and HRE groups were significantly increased compared to the HD group (p < 0.05). The mRNA expressions of TLR4, ICAM-1 and VCAM-1, which induce pro-inflammatory cytokine production, were strongly decreased in the HR, HE, and HRE groups compared to the HD group.[Conclusion] These results suggest that moderate exercise training inhibits macrophage infiltration and up regulation of CD163 expression. However, resveratrol supplementation is not enough to ameliorate obesity-induced macrophage infiltration and switching.

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Section: Discussionmentioning

confidence: 99%

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

Jeong

Lee

Park

et al. 2015

J. Exerc. Nutr, Biochem.

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show abstract

“…In the field of obesity and related metabolic alterations, some of these polyphenols have shown a clear anti-obesity action in animal models. One of the most frequently studied molecules is resveratrol, which belongs to the group of stilbenes (Ahn et al 2008a;Baile et al 2011;Baur et al 2006;DalPan et al 2010;Macarulla et al 2009;Rivera et al 2009;Szkudelska et al 2009). With regard to flavonoids, and more specifically to quercetin, little is known of any potential role in obesity prevention or treatment.…”

Section: Discussionmentioning

confidence: 99%

Quercetin can reduce insulin resistance without decreasing adipose tissue and skeletal muscle fat accumulation

et al. 2013

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Quercetin exhibits a wide range of biological functions. The first aim of the present work was to analyze the effects of quercetin on fat accumulation in adipose tissue and glycemic control in rats. Any potential involvement of muscle fatty acid oxidation in its effect on glycemic control was also assessed. Animals were fed a high-fat high-sucrose diet either supplemented with quercetin (30 mg/kg body weight/day), or not supplemented, for 6 weeks. One week before killing, a glucose tolerance test was carried out. Muscle triacylglycerol content, serum glucose, insulin, fructosamine and free fatty acids were measured, and homeostatic model assessment for insulin resistance (HOMA-IR) was calculated. The activities of lipogenic enzymes and lipoprotein lipase in adipose tissue, carnitine palmitoyl transferase-1b (CPT-1b) and citrate synthase in skeletal muscle, and the expression of several genes, ACO, CD36, CPT-1b, PPAR-a, PGC-1a, UCP 3 , TFAM and COX-2 in skeletal muscle were analyzed. Quercetin caused no significant reduction in body weight or adipose tissue sizes. However, fructosamine, basal glucose and insulin, and consequently HOMA-IR, were significantly reduced by quercetin. No changes were observed in the activity of lipogenic enzymes and lipoprotein lipase. Muscle triacylglycerol content was similar in both experimental groups. The expression of ACO, CD36, CPT-1b, PPAR-a, PGC-1a, UCP 3 , TFAM and COX-2 remained unchanged. It can be concluded that quercetin is more effective as an anti-diabetic than as an anti-obesity biomolecule. The improvement in insulin resistance induced by this flavonoid is not mediated by a delipidating effect in skeletal muscle.

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“…The mRNA expressions were examined with ABI software and calculated with the 2-△△Ct, as previous reports (Livak & Schmittgen, 2001). Hepatic lipid content was analysed as described by Ahn et al (2008). Briefly, portions of liver samples from each pig were weighed and homogenized in Tris-HCl solution with a glass Dounce homogenizer on ice.…”

Section: Methodsmentioning

confidence: 99%

Altered tissue mineralization, increased hepatic lipid and inhibited autophagy in intrauterine growth retardation piglets

Wang¹,

Zheng²,

Siyal³

et al. 2018

SA J. An. Sci.

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Mineral homeostasis is often disrupted in intrauterine growth retardation (IUGR) infants. Most studies focus on calcium or phosphorus metabolism of IUGR infants via determining serum mineral concentrations instead of tissues. This study was conducted to investigate the effects of IUGR on the mineralization and physiological functions of tissue in a piglet model. Six normal birth weight (NBW) and six IUGR neonatal piglets were slaughtered at 35 days. Mineral concentrations in blood and selected tissues (liver, kidney, lungs, heart, and longissimus dorsi muscle (LDM)), hepatic lipid, mRNA expressions of magnesium (Mg) metabolism, and autophagy were analysed. Results showed that IUGR pigs showed significantly lower phosphorus (P) in LDM, and lower Mg in the liver and LDM, and higher Mg in lungs than NBW pigs. There were no significant differences in concentrations of selenium (Se), calcium (Ca), copper (Cu), aluminium (Al), and lithium (Li) in selected tissues. IUGR pigs had similar mRNA expression of TRPM7 and MagT1 to NBW pigs, but significantly lower expressions of HNF1B and Mrs2 in the liver than NBW pigs. Hepatic triglyceride was significantly increased, and MAP1LC3B expression was significantly decreased in IUGR pigs compared with those of NBW pigs. These result suggested that IUGR pigs had tissue mineralization disturbance, especially for Mg, and liver dysfunction (increased hepatic lipid and inhibited autophagy). Hepatic Mg deficiency might result from increased Mg efflux via reducing HNF1B expression. ______________________________________________________________________________________

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Dietary resveratrol alters lipid metabolism-related gene expression of mice on an atherogenic diet

Cited by 161 publications

References 42 publications

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

The effects of either resveratrol or exercise on macrophage infiltration and switching from M1 to M2 in high fat diet mice

Quercetin can reduce insulin resistance without decreasing adipose tissue and skeletal muscle fat accumulation

Altered tissue mineralization, increased hepatic lipid and inhibited autophagy in intrauterine growth retardation piglets

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