Dietary Resveratrol Alleviates AFB1-Induced Ileum Damage in Ducks via the Nrf2 and NF-κB/NLRP3 Signaling Pathways and CYP1A1/2 Expressions

Abstract

The aim of this study was to explore the mechanism underlying the protective effects of resveratrol against Aflatoxin B1-induced ileum injury in ducks. A corn–soybean meal-basal diet and two test diets (500 mg/kg resveratrol +0.2 mg Aflatoxin B1/kg, 0.2 mg AFB1/kg) were used in a 10-wk design trial (n = 15 ducks/group). These results showed that the toxicity of Aflatoxin B1 significantly reduced the antioxidant capacity of duck ileum and induced inflammation, oxidative stress, mitochondrial dysfunction and DNA damage in ducks. The expression of genes, including CYP1A2, CYP2A6, and CYP3A4, at the mRNA level was significantly upregulated (p < 0.05) by AFB1. The level of Nrf2 was suppressed (p < 0.05) and the mRNA and protein level of NF-κB was activated (p < 0.05) in the AFB1 group. However, supplementation with 500 mg/kg dietary resveratrol in Aflatoxin B1-induced ducks significantly ameliorated these alterations and decreased the mRNA expression of CYP1A1 and CYP1A2 (p < 0.05) and the production of AFB1-DNA adducts (p < 0.05). The results proved that resveratrol alleviated ileum injury induced by AFB1, decreased the production of AFB1-DNA adducts by downregulating the expression of CYP1A1 and CYP1A2, and reduced DNA damage and oxidative stress via the Nrf2/ Keap1 and NF-κB/NLRP3 signaling pathways.

Alleviation of Oral Exposure to Aflatoxin B1-Induced Renal Dysfunction, Oxidative Stress, and Cell Apoptosis in Mice Kidney by Curcumin

“…AFB1 is the most toxic of many secondary metabolites produced by fungi, posing considerable health risks to people and animals due to its hepatotoxic, immunotoxic, carcinogenic, and teratogenic effects [ 30 , 31 , 32 ]. The role of oxidative stress associated with AFB1-mediated organ toxicity has been widely researched in animal models [ 11 , 33 , 34 ].…”

Effect of Porcine Clostridium perfringens on Intestinal Barrier, Immunity, and Quantitative Analysis of Intestinal Bacterial Communities in Mice

“…Total RNA isolation from the tissues was carried out according to previous studies ( 22 , 23 ), using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA) according to the manufacturer's instructions. NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA) was used to measure the optical density at 260 to 280 and quantified the purity of the RNA.…”

Nutrigenomics in livestock sector and its human-animal interface-a review

“…In chicks fed aflatoxin B1, turmeric blocked the biotransformation expression of antioxidants and immune system genes ( Muhammad et al., 2018 ). Dietary aflatoxin B1 (AFB1) alters serum biochemical parameters, decreases liver anti-oxidative enzymes, suppresses Nrf2 and phase-II enzymes involved in AFB1 detoxification, and significantly increases CYP enzyme expression levels involved in AFB1 biotransformation ( Yang et al., 2022 ).…”