Dietary regulation of Ucp2 and Ucp3 expressions in white adipose tissues of beef cattle

Shigematsu, Mei; Yamada, Tomoya; Wong, Yun Yi; Kanamori, Yohei; Murakami, Masaru; Fujimoto, Yusuke; Suzuki, Mika; Kida, Ryosuke; Qiao, Yuhang; Tomonaga, Shozo; Matsui, Tohru; Funaba, Masayuki

doi:10.1139/cjas-2016-0020

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Since the regulation of gene transcription is generally cell‐type dependent, the present results are likely in part, a reflection of the regulation of Ucp1 in myogenic cells. In fact, we previously showed that diet‐related changes in Ucp1 expression were closely related to those in Ucp2 expression in bovine fat 25,33,41 . In the current study, we found no similar changes between Ucp1 expression and Ucp2 expression in response to A‐83‐01 and LDN‐193189 treatments in myogenic cells.…”

Section: Resultscontrasting

confidence: 84%

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Regulatory expression of uncoupling protein 1 and its related genes by endogenous activity of the transforming growth factor‐β family in bovine myogenic cells

Eldaim

Zhao

Murakami

et al. 2020

Cell Biochemistry & Function

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Uncoupling protein 1 (UCP1) is responsible for non‐shivering thermogenesis, with restricted expression in brown/beige adipocytes in humans and rodents. We have previously shown an unexpected expression of UCP1 in bovine skeletal muscles. This study evaluated factors affecting Ucp1 gene expression in cultured bovine myogenic cells. Myosatellite cells, which were isolated from the bovine musculus longissimus cervicis, were induced to differentiate into myotubes in the presence of 2% horse serum. Previous studies using murine brown/beige adipocytes revealed that Ucp1 expression levels are directly increased by forskolin and all‐trans retinoic acid (RA). The transforming growth factor‐β (TGF‐β)/activin pathway negatively regulated Ucp1 expression, whereas activation of the bone morphogenetic protein (BMP) pathway indirectly increases Ucp1 expression through the stimulation of brown/beige adipogenesis. Neither forskolin nor RA significantly affected Ucp1 mRNA levels in bovine myogenic cells. A‐83‐01, an inhibitor of the TGF‐β/activin pathway, stimulated myogenesis in these cells. A‐83‐01 significantly increased the expression of some brown fat signature genes such as Pgc‐1α, Cox7a1, and Dio2, with a quantitative but not significant increase in the expression of Ucp1. Treatment with LDN‐193189, an inhibitor of the BMP pathway, did not affect the differentiation of bovine myosatellite cells. Rather, LDN‐193189 increased Ucp1 mRNA levels without modulating the levels of other brown/beige adipocyte‐related genes. The current results indicate that the regulation of Ucp1 expression in bovine myogenic cells is distinct from that in murine brown/beige adipocytes, which has been more intensely characterized. Significance of the study We previously reported unexpected expression of Ucp1 in bovine muscle tissues; Ucp1 expression has been known to be detected predominantly in brown/beige adipocytes. This study examined regulatory expression of bovine Ucp1 in myogenic cells. Consistent with the changes in expression levels of brown/beige adipocyte‐selective genes, Ucp1 expression tended to be increased by inhibition of endogenous TGF‐β activity. In contrast, inhibition of endogenous BMP significantly increased Ucp1 expression without affecting brown/beige adipocyte‐selective gene expression. The current results indicate that regulatory expression of Ucp1 in bovine myogenic cells is distinct from that in murine brown/beige adipocytes that is more intensely characterized.

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Section: Resultscontrasting

confidence: 84%

“…We previously showed a close relationship between Ucp1 expression and Ucp2 expression in bovine fat 33 . PPARγ stimulates transcription of murine Ucp1 and Ucp2 34,35 .…”

Section: Resultsmentioning

confidence: 90%

Regulatory expression of uncoupling protein 1 and its related genes by endogenous activity of the transforming growth factor‐β family in bovine myogenic cells

Eldaim

Zhao

Murakami

et al. 2020

Cell Biochemistry & Function

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“…The UCP2 gene is almost ubiquitously expressed throughout body tissues [49]. It is regulated by TR and PPARγ and was recently shown to be regulated by diet in subcutaneous white adipose tissue of cattle [50]. However, our heifers had been offered the same diet for 6 mo before PREG biopsises were taken.…”

Section: Resultsmentioning

confidence: 99%

Long-term effects of prior diets, dietary transition and pregnancy on adipose gene expression in dairy heifers

et al. 2019

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Adipose tissue is highly involved in whole-body metabolism and is the main site for lipid synthesis, storage and mobilization in ruminants. Therefore, knowledge about adipose tissue responses to different diets is important, especially in growing heifers as the feeding regimes of replacement heifers affect their future success as dairy cows. However, at gene expression level such knowledge is limited. As part of a larger feed trial, adipose tissue biopsies from 24 Norwegian Red heifers were collected at 12 months of age (12MO) and at month seven of gestation (PREG) and analyzed by next-generation mRNA sequencing. Between these two sampling points, all heifers had gone through a successful conception and a feed change from four dietary treatments of high or low energy (HE/LE) and protein (HP/LP) content (treatments LPHE, HPHE, LPLE and HPLE) to a low-energy, low-protein pregnancy feed given to all animals. Gene expression differences between different feed treatments at 12MO are described in an earlier publication from our group. The main objectives of this study were to investigate the long-term effects of diets differing in protein and energy density level on gene expression in adipose tissue of growing replacement dairy heifers. To achieve this, we examined the post-treatment effects between the treatment groups at month seven of gestation; 6 months after the termination of experimental feeding, and the long-term gene expression changes occurring in the adipose tissue between 12MO and PREG. Post-treatment group comparisons showed evidence of long-term effects of dietary treatment on adipose gene expression. Differences between protein treatments were smaller than between energy treatments. Adipose gene expression changes from 12MO to PREG were much larger for the HE than the LE treatments and seemed to mostly be explained by the characteristics of the diet change. 97 genes displayed a unidirectional expression change for all groups from 12MO to PREG, and are considered to be treatment-independent, possibly caused by pregnancy or increased age. This study provides candidate genes and key regulators for further studies on pregnancy preservation (TGFB1, CFD ) and metabolic regulation and efficiency (PI3K, RICTOR, MAP4K4,) in dairy cattle.

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Relationships between expression levels of genes related to adipogenesis and adipocyte function in dogs

et al. 2019

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Dietary regulation of Ucp2 and Ucp3 expressions in white adipose tissues of beef cattle

Cited by 5 publications

References 15 publications

Regulatory expression of uncoupling protein 1 and its related genes by endogenous activity of the transforming growth factor‐β family in bovine myogenic cells

Regulatory expression of uncoupling protein 1 and its related genes by endogenous activity of the transforming growth factor‐β family in bovine myogenic cells

Long-term effects of prior diets, dietary transition and pregnancy on adipose gene expression in dairy heifers

Relationships between expression levels of genes related to adipogenesis and adipocyte function in dogs

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