Dietary Polyunsaturated Fatty Acids and Cellular Immune Response in Broiler Chickens

Abstract

immune organ weights as a general indicator, haemocytometric measurements, intestinal microbial count and hindgut acidosis, hindgut volatile fatty acids, and cellular immune response using PHA-test. The use of the different dietary treatments did not affect the general health status of the chickens and the mortality was minimal with no signs of illness or outbreaks. At 3-wk of age, there was no significant effect observed in the microbial counts of the intestine. However, at 5-wk of age, the highest microbial count was significantly observed for broilers fed EO (7.30%), closely followed by SFO (6.95%) and the least microbial counts were observed for CO (5.63%). No significance was observed for LAB and Salmonella. There was no significance observed for the effect of the dietary treatments on the hindgut volatile acid in the broilers. Wattle swelling changes were significant between dietary treatments. The results revealed that dietary FLO, FO, and DHA oils induced higher cellular response than the other treatments (P=0.035), representing higher cellular response in these groups. In conclusion, supplemental oils rich in n-3 fatty acids may enhance the immune response in broiler chickens, represented by the intestinal microbial counts and the cellular immune response.

Dietary Supplementation of Fructooligosaccharides Enhanced Antioxidant Activity and Cellular Immune Response in Broiler Chickens

“…The collected crude extracts were used directly for microbial analysis. LAB, E. coli , and Salmonella counts were determined using standard microbiological methods, as described by Lorch ( 38 ) and Al-Khalaifah et al ( 1 ), and the samples were analyzed by applying spreading technology. E. coli and Salmonella count experiments were conducted using Brilliance E. Coli selective and Xylose-Lysine-Desoxycholate agar media (Oxoid), respectively, while LAB experiments were conducted using de Man, Rogosa, Sharpe (MRS) media (oxoid).…”

“…Keeping the health status of poultry flocks at optimum levels is essential to improve the poultry industry and to ensure food security, especially during global pandemics such as the current coronavirus crisis. Using naturally occurring feed ingredients is one way to achieve this goal ( 1 – 4 ). Prebiotics are short-chain oligosaccharide components that are indigestible and trigger the growth and/or activity of beneficial gastrointestinal microbiota in the digestive system.…”

Evaluation of the lactic acid bacteria based formulated probiotic product for poultry

“…The collected crude extracts were used directly for microbial analysis. The LAB, E. coli, and Salmonella counts were determined using standard microbiological methods, as described by Lorch (1995) and Al-Khalaifah et al (2021), and the samples were analyzed by applying the spreading technique. The E. coli and Salmonella count experiments were conducted using the Brilliance E. coli selective and the Xylose-Lysine-Desoxycholate agar media (Oxoid), respectively, while the LAB experiments were conducted using the de Man, Rogosa, Sharpe (MRS) medium (oxoid).…”