Dietary Polyunsaturated Fatty Acid Supplementation Improves the Quality of Stallion Cryopreserved Semen

Rodrigues, Paula Gomes; Moura, Regina; Rocha, Luiz Célio Souza; Bottino, M. P.; Nichi, Marcílio; Maculan, Renata; Bertechini, Antônio Gilberto; Souza, José Camisão de

doi:10.1016/j.jevs.2016.08.007

Cited by 11 publications

(7 citation statements)

References 32 publications

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“…Because the cholesterol to phospholipids proportion cannot be altered in sperm due to its dependence on species’ genetic factor [32], addition of PUFAs to diets has been suggested in order to increase its proportion relative to sperm membrane phospholipids and improve its fluidity and resistance to thermal stress [33]. Moreover, dietary fatty acids supplementation has been reported to improve the cryopreserved stallion semen [34]. We have also shown in the very recent publication from our laboratory that fatty acids could be responsible for the spermatoprotective effect of Telfairia occidentalis leaves in rats [35].…”

Section: Discussionmentioning

confidence: 99%

Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

Alagbonsi

Olayaki

Abdulrahim

et al. 2019

BMC Complement Altern Med

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Background Nigerian Cannabis sativa (hemp) causes male gonadotoxicity by inducing hyperprolactinemia, down-regulation of hypothalamic-pituitary-testicular axis, and oxidative stress. Benin republic hemp has been preferred by illicit users in Nigeria but its effect on male fertility is not understood. This study determined and compared the compositions of Benin republic hemp ethanol extract (BHE) and Nigerian hemp. The effects of BHE on semen parameters, reproductive hormones, and anti-oxidant status, and the possibility of bromocriptine (prolactin inhibitor) to abolish hemp-induced toxicities in rats were also investigated. Methods Thirty-six male Wistar rats were blindly randomized into 6 oral treatment groups ( n = 6 each). Groups I (control) and II received normal saline and bromocriptine (3 mg/kg) respectively. Groups III and IV received 2 mg/kg of BHE alone and in combination with bromocriptine respectively, while groups V and VI received 10 mg/kg BHE alone and in combination with bromocriptine respectively. Comparisons among the groups were done by one-way analysis of variance, followed by post-hoc Tukey multiple comparison test. Statistical significance was considered at p < 0.05. Results The BHE has no cannabichromene and tetrahydrocannabinol but a very small quantity of cannabinol and higher quantity of fatty acids when compared to Nigerian hemp. Both doses of BHE increased sperm count, morphology and viability but not motility. Co-administration of BHE with bromocriptine lowered sperm count but increased sperm morphology and viability. Bromocriptine and/or BHE caused reduction in the plasma prolactin level, increase in the plasma superoxide dismutase activity, but no significant change in the plasma gonadotropin releasing hormone, follicle stimulating hormone (except for the increase in rats that received bromocriptine+ 10 mg/kg BHE), luteinizing hormone, estradiol, malondialdehyde and glutathione peroxidase. The 10 mg/kg BHE or bromocriptine+BHE (both doses) increased total anti-oxidant capacity and catalase. Conclusions The BHE improves semen parameters by reducing plasma prolactin and enhancing plasma anti-oxidant status. Its pro-fertility potential might be associated with its deficiency in the widely known gonadotoxic phytocannabinoids.

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Section: Discussionmentioning

confidence: 99%

Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

Alagbonsi

Olayaki

Abdulrahim

et al. 2019

BMC Complement Altern Med

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“…Rodrigues and collaborators (Rodrigues et al., 2017 ) assessed the effects of linseed oil supplementation, as a source of DHA, on the quality of fresh, cooled and frozen‐thawed semen from ten Mangalarga Marchador stallions. In this cross‐over study, horses from the experimental group received 150 ml of linseed‐based oil supplement to their diet for 60 days and, after a sixty‐day washout period, groups were reversed.…”

Section: Introductionmentioning

confidence: 99%

Use of nutraceuticals in the stallion: Effects on semen quality and preservation

Bazzano

Laus

Spaterna

et al. 2021

Reprod Domestic Animals

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Nutritional supplements are widely used in the equine industry with the aim of improving horse health, sports or reproductive performances. Over the years, a number of studies have focused on investigating the effects of several dietary compounds on the quality and preservation of stallion semen. This paper reviews the literature available on the use of nutritional supplementation for the improvement of reproductive performance and semen quality in equine species, critically appraising the benefits and negative effects of several compounds found in complementary feeds such as PUFAs from different sources, vitamins and antioxidants, carnitine and botanical extracts. Different nutraceuticals have been highlighted to improve stallion fertility by providing optimal levels of antioxidants, with the most promising results obtained by the combination of PUFAs and antioxidants that resulted to be essential for the maintenance of normal reproductive functions and the reduction of cryodamage in cooled and frozen equine semen.

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“…Animals cannot synthesize n-6 and n-3 fatty acids de novo owing to a lack of appropriate fatty acid desaturase enzymes and the need to obtain these fatty acids from their diet (Wathes et al, 2007). In addition, dietary FA are known to affect the FA composition of sperm membranes in humans (Conquer et al, 2000), bulls (Moallem et al, 2015), stallions (Brinsko et al, 2005;Rodrigues et al, 2017), pigs (Cook & McMaster, 2002) and roosters (Cerolini et al, 2006). In many mammalian species, the plasma membrane has more than 60% polyunsaturated fatty acids (PUFAs), although sperm membrane lipids vary between species (Saether et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Persia fat ® (PF) is an Iranian product with the same concentration of n-3 and n-6 FA as supplementary fat. Many studies have demonstrated that dietary n-3 and n-6 fatty acids enhance the preservation of spermatozoa after cold shock and ice crystal formation in humans (Vireque et al, 2016), bulls (Gholami et al, 2010;Khoshvaght et al, 2016), goats (Dolatpanah et al, 2008), stallions (Rodrigues et al, 2017) and roosters (Cerolini et al, 2006). On the other hand, Karimi et al (2017) demonstrated that supplementation of the diet of bulls with conjugated linoleic acid (CLA) did not affect fresh and frozen semen quality.…”

Section: Introductionmentioning

confidence: 99%

Influence of various dietary fat sources on freezing capacity of Moghani ram semen

Hedayat-Evrigh¹,

Moradi²,

Vahedi³

et al. 2019

SA J. An. Sci.

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The aim of this study was to investigate the effects of dietary supplementation of protected fish oil (FO) and Persia fat ® (PF) on the quality of Moghani ram semen. For this purpose, a total of 96 ejaculates were collected from 12 healthy mature Moghani rams, which were divided into three distinct groups (n = 4) and were assigned to one of three experimental diets. The first group (control) received a diet supplemented with palm oil (PO), while the second and third groups received encapsulated FO and PF, respectively. After primary evaluation, semen samples of each group were pooled to eliminate individual differences, and then evaluated for semen concentration and volume. Afterwards, the samples were diluted with a Tris-based extender and frozen with a standard protocol. After thawing, motion kinetics, viability, membrane functionality and abnormality were assessed. The results showed that the group that received FO had significanty higher viability (quadratic), progressive motility (PM) (%), average path velocity (VAP) (µm/s), curvilinear velocity (VCL) (µm/s) (linear), amplitude of lateral head displacement (ALH) (µm) (quadratic) and sperm concentration (linear) than the others. Additionally, total motility (TM) (%) and straight-line velocity (VSL) (µm/s) were significantly higher in the groups that received FO and PF compared with the control (quadratic) The results indicated that sperm abnormalities in the control group were significantly higher than the other groups. In conclusion, enrichment of the diet with FO or Persia fat could enhance ram sperm quality after freeze-thawing process.

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Dietary Polyunsaturated Fatty Acid Supplementation Improves the Quality of Stallion Cryopreserved Semen

Cited by 11 publications

References 32 publications

Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

Cannabinoid-deficient Benin republic hemp (Cannabis sativa L.) improves semen parameters by reducing prolactin and enhancing anti-oxidant status

Use of nutraceuticals in the stallion: Effects on semen quality and preservation

Influence of various dietary fat sources on freezing capacity of Moghani ram semen

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