Dietary Plasma Protein Affects the Immune Response of Weaned Rats Challenged with S. aureus Superantigen B

Abstract

The aim of this study was to determine the potential modulatory effects of diets supplemented with spray-dried animal plasma (SDAP) or immunoglobulin concentrates (IC) on the immune response of rats challenged with Staphylococcus aureus enterotoxin B (SEB). Lewis rats were fed diets containing 80 g of SDAP/kg diet, 22.7 g of IC/kg diet, or milk proteins (Control diet) from postnatal d 21 (weaning) for 14 d. On d 30 and 33, rats were given SEB (0.5 mg/kg body weight; i.p.). Organized gut-associated lymphoid tissue (GALT) populations, intestinal secretion, mucosal and serum immunoglobulin concentrations, and neutrophil infiltration were studied. On d 35, blood was collected under anesthesia and samples of intestinal mucosa, Peyer's patches, mesenteric lymph nodes (MLN), and spleen were taken. SEB increased the water content of feces, which was prevented by diets containing either SDAP (P < 0.002) or IC (P < 0.001), indicating that plasma protein-supplemented diets can reverse the SEB-induced secretory response. In Peyer's patches, the diet containing SDAP partially prevented the SEB-induced increase in T lymphocytes (P < 0.1) and reduced the percentage of activated T helper cells (P < 0.05). In MLN, activated T lymphocytes were increased by SEB but they were not affected by diet. No effects of SEB or dietary supplementation on mucosal IgA and serum IgA and IgG were observed. The effects of SDAP supplementation on the lymphocyte populations of GALT in rats challenged with SEB support the view that SDAP can modulate the immune response and suggest that plasma protein supplementation can prevent GALT from possible activation by luminal bacterial superantigens.

Lemon Verbena Infusion Consumption Attenuates Oxidative Stress in Dextran Sulfate Sodium-Induced Colitis in the Rat

“…(T c ) cells. A similar trend was observed in inflamed rats exposed to staphylococcal enterotoxin [48]. Lemon verbena infusion consumption tends to counteract the modifications in immune cell subsets observed during colitis.…”

Effects of Yeast-Derived ProteinvsSpray-Dried Porcine Plasma Supplementation on Growth Performance, Metabolism and Immune Response of Weanling Piglets

“…Furthermore, energy metabolism status of piglets fed SDPP diet may be optimised, as indicated by the lower serum level of TG (-29%, P<0.05) compared to pigs fed control diet (Table 5), which may be also associated with metabolism of ingested fat (Jones et al, 1992). In addition, the SDPP can alleviate the activation of gut-associated lymphatic tissue system and systematic immune cells, thus allowing more energy for growth (Demas et al, 1997;Perez-Bosque et al, 2004). In our previous study, serum level of cortisol has been successfully measured to reflect the stress-relief effect of dietary treatments in weaning piglets (Che et al, 2012).…”

Substitution of blood plasma with soy protein concentrate in piglet diet

“…As described by Meyer and Harvey (2004), the cell counts in the present study are within standard counts for swine. Furthermore, plasma can relieve the activation of the lymphatic tissue system associated with the intestine and cells from the systemic immune system, allowing more energy for growth (Demas et al, 1997;Perez-Bosque et al, 2004). The weaning process, along with its challenges and changes, is associated with a greater activation of the immune system in piglets, which is generally associated with intestinal inflammation and a systemic proinflammatory response with increased production of defense cells and cytokines (Moretó and Pérez-Bosque, 2009).…”