Dietary Pattern Specific Protein Biomarkers for Cardiovascular Disease: A Cross‐Sectional Study in 2 Independent Cohorts

Lemming, Eva Warensjö; Byberg, Liisa; Stattin, Karl; Ahmad, Shafqat; Lind, Lars; Elmståhl, Sölve; Larsson, Susanna C.; Wolk, Alicja; Michaëlsson, Karl

doi:10.1161/jaha.118.011860

Cited by 25 publications

(19 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To the best of our knowledge, only two prior studies have examined proteomic profiles of dietary patterns [38,39] Prior experimental studies indicate that gene and/or protein expression of ERBB1, PDL2, and CNTN1 is atheroprotective [40][41][42]. Further, multiple studies have reported that low concentrations of IGEBP1 are directly associated with glucose intolerance and risk of diabetes mellitus [43,44].…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Metabolomic Correlates of Healthy Dietary Patterns: The Framingham Heart Study

Walker

Song

et al. 2020

Nutrients

View full text Add to dashboard Cite

Data on proteomic and metabolomic signatures of healthy dietary patterns are limited. We evaluated the cross-sectional association of serum proteomic and metabolomic markers with three dietary patterns: the Alternative Healthy Eating Index (AHEI), the Dietary Approaches to Stop Hypertension (DASH) diet; and a Mediterranean-style (MDS) diet. We examined participants from the Framingham Offspring Study (mean age; 55 years; 52% women) who had complete proteomic (n = 1713) and metabolomic (n = 2284) data; using food frequency questionnaires to derive dietary pattern indices. Proteins and metabolites were quantified using the SomaScan platform and liquid chromatography/tandem mass spectrometry; respectively. We used multivariable-adjusted linear regression models to relate each dietary pattern index (independent variables) to each proteomic and metabolomic marker (dependent variables). Of the 1373 proteins; 103 were associated with at least one dietary pattern (48 with AHEI; 83 with DASH; and 8 with MDS; all false discovery rate [FDR] ≤ 0.05). We identified unique associations between dietary patterns and proteins (17 with AHEI; 52 with DASH; and 3 with MDS; all FDR ≤ 0.05). Significant proteins enriched biological pathways involved in cellular metabolism/proliferation and immune response/inflammation. Of the 216 metabolites; 65 were associated with at least one dietary pattern (38 with AHEI; 43 with DASH; and 50 with MDS; all FDR ≤ 0.05). All three dietary patterns were associated with a common signature of 24 metabolites (63% lipids). Proteins and metabolites associated with dietary patterns may help characterize intermediate phenotypes that provide insights into the molecular mechanisms mediating diet-related disease. Our findings warrant replication in independent populations

show abstract

Section: Discussionmentioning

confidence: 99%

Proteomic and Metabolomic Correlates of Healthy Dietary Patterns: The Framingham Heart Study

Walker

Song

et al. 2020

Nutrients

View full text Add to dashboard Cite

show abstract

“…In the present study, we observed diet‐protein associations consistent with those reported in 3 prior studies. 32 , 33 , 34 For example, the unhealthy dietary patterns characterized in these studies were associated with higher concentrations of PAI1 (plasminogen activator inhibitor 1), MPO (myeloperoxidase), APOB (apolipoprotein B), GDF15, and HPX (hemopexin). We found that higher diet quality scores, reflecting healthier diet, were associated with lower levels of these proteins.…”

Section: Discussionmentioning

confidence: 87%

“… 1 Although the association between diet and protein biomarkers has not been well studied, we observed associations similar to those reported in previous studies. 32 , 33 The cross‐sectional Toronto Nutrigenomics and Health study demonstrated that a Western‐style dietary pattern was associated with 25 proteins involved in coagulation and lipid metabolism among 54 putative CVD biomarker proteins. 32 A Swedish study of 2 population‐based cohorts analyzed 184 CVD‐related circulating proteins and demonstrated that dietary patterns were associated with 21 proteins.…”

Section: Discussionmentioning

confidence: 99%

“… 32 A Swedish study of 2 population‐based cohorts analyzed 184 CVD‐related circulating proteins and demonstrated that dietary patterns were associated with 21 proteins. 33 These dietary pattern‐associated proteins are involved in multiple pathways such as inflammation and lipid metabolism. In the present study, we observed diet‐protein associations consistent with those reported in 3 prior studies.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Proteins as Mediators of the Association Between Diet Quality and Incident Cardiovascular Disease and All‐Cause Mortality: The Framingham Heart Study

Kim

et al. 2021

JAHA

View full text Add to dashboard Cite

Background Biological mechanisms underlying the association of a healthy diet with chronic diseases remain unclear. Targeted proteomics may facilitate the understanding of mechanisms linking diet to chronic diseases. Methods and Results We examined 6360 participants (mean age 50 years; 54% women) in the Framingham Heart Study. The associations between diet and 71 cardiovascular disease (CVD)‐related proteins were examined using 3 diet quality scores: the Alternate Healthy Eating Index, the modified Mediterranean‐style Diet Score, and the modified Dietary Approaches to Stop Hypertension diet score. A mediation analysis was conducted to examine which proteins mediated the associations of diet with incident CVD and all‐cause mortality. Thirty of the 71 proteins were associated with at least 1 diet quality score ( P <0.0007) after adjustment for multiple covariates in all study participants and confirmed by an internal validation analysis. Gene ontology analysis identified inflammation‐related pathways such as regulation of cell killing and neuroinflammatory response (Bonferroni corrected P <0.05). During a median follow‐up of 13 years, we documented 512 deaths and 488 incident CVD events. Higher diet quality scores were associated with lower risk of CVD ( P ≤0.03) and mortality ( P ≤0.004). After adjusting for multiple potential confounders, 4 proteins (B2M [beta‐2‐microglobulin], GDF15 [growth differentiation factor 15], sICAM1 [soluble intercellular adhesion molecule 1], and UCMGP [uncarboxylated matrix Gla‐protein]) mediated the association between at least 1 diet quality score and all‐cause mortality (median proportion of mediation ranged from 8.6% to 25.9%). We also observed that GDF15 mediated the association of the Alternate Healthy Eating Index with CVD (median proportion of mediation: 8.6%). Conclusions Diet quality is associated with new‐onset CVD and mortality and with circulating CVD‐related proteins. Several proteins appear to mediate the association of diet with these outcomes.

show abstract

“…When compared with national data, the full cohort well represents the Swedish population in terms of age distribution, educational level, prevalence of overweight and obesity, and smoking status ( 12 ) and the subcohort has similar distributions of these characteristics with the exception of an on average higher educational level. ( 13‐15 )…”

Section: Methodsmentioning

confidence: 99%

Serum 25-hydroxyvitamin D is associated with fracture risk only during periods of seasonally high levels in women with a high body mass index

Michaëlsson

Byberg

Svennblad

et al. 2020

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

Serum 25-hydroxyvitamin D (S-25OHD) is used to assess vitamin D status and is known to be affected by season and fat mass. Because these factors are often ignored when interpreting S-25OHD, assessment of vitamin D associations with disease outcomes may be distorted. We aimed to investigate the impact of season of blood draw and fat mass on the association of S25OHD with fracture risk. We enrolled 5000 women, mean AE SD age 68 AE 7 years, with dual-energy x-ray absorptiometry (DXA) scans and blood collection in a population-based cohort. Proportional hazards regression, stratified by season and fat mass, was used to determine hazard ratios (HRs) of fracture according to categories of S-25OHD. Our secondary exposures were serum 1,25-dihydroxycholecalciferol (1,25-(OH) 2 D 3 ), the most active vitamin D metabolite and plasma parathyroid hormone (P-PTH). During an average of 9.2 years of follow-up, 1080 women had a fracture. Women with S-25OHD <30 nmol/L drawn during sunny months (May-October) had a multivariable-adjusted fracture HR of 2.06 (95% CI, 1.27-3.35) compared with those with S-25OHD >60 nmol/L; those with S-25OHD 30-40 nmol/L had an HR of 1.59 (95% CI, 1.12-2.26). In contrast, S-25OHD drawn during November through April was unrelated to fracture risk. The increased risk with low sunny season S-25OHD was seen only among women with body mass index (BMI) ≥25 kg/m 2 or fat mass index (FMI) ≥9.8 kg/m 2 . High fat mass and low S-25OHD were independently related to lower S-1,-25-dihydroxycholecalciferol, which itself predicted fracture risk with samples collected during the sunny season. Irrespective of season, P-PTH was unrelated to fracture risk. We conclude that S-25OHD is associated with fracture risk only if drawn during periods of seasonally high levels in women with a high BMI. These results have implications for the evaluation of vitamin D status and can explain the lack of effect seen with vitamin D supplementation in many fracture trials.

show abstract

Dietary Pattern Specific Protein Biomarkers for Cardiovascular Disease: A Cross‐Sectional Study in 2 Independent Cohorts

Cited by 25 publications

References 50 publications

Proteomic and Metabolomic Correlates of Healthy Dietary Patterns: The Framingham Heart Study

Proteomic and Metabolomic Correlates of Healthy Dietary Patterns: The Framingham Heart Study

Proteins as Mediators of the Association Between Diet Quality and Incident Cardiovascular Disease and All‐Cause Mortality: The Framingham Heart Study

Serum 25-hydroxyvitamin D is associated with fracture risk only during periods of seasonally high levels in women with a high body mass index

Contact Info

Product

Resources

About