Dietary of Lactobacillus paracasei and Bifidobacterium longum improve nonspecific immune responses, growth performance, and resistance against Vibrio parahaemolyticus in Penaeus vannamei

Huang, Huai-Ting; Hu, Yeh-Fang; Lee, Bao-Hong; Huang, Chih‐Yang; Lin, Yu-Ru; Huang, Shu-Ning; Chen, Yin-Yu; Chang, Jui-Jen; Nan, Fan-Hua

doi:10.1016/j.fsi.2022.07.062

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…Dewi et al [10] reported that a guava (P. guajava) leaf extract enhanced the PO activity, phagocytic activity, and O 2 − production of whiteleg shrimp hemocytes in in vitro experiments. The phagocytic activity, O 2 − production, and PO activity of whiteleg shrimp hemocytes increased after an in vitro culture with a hot-water extract of Bidens alba and P. amboinicus [11]. Therefore, this in vitro hemocyte experiment was performed to determine whether hydrolyzable tannins affect the nonspecific immunity of shrimp.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, plants are safe and have minimal side effects on aquatic animals [8]. Many studies have reported that plants, such as Moringa oleifera leaves, Psidium guajava leaves, Bidens alba, and Plectranthus amboinicus, can be used to promote growth and enhance immunity and resistance to pathogens for whiteleg shrimp [9][10][11][12]. Plants synthesize aromatic substances, such as terpenoids, tannins, and quinones [13].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Supplementation with Hydrolyzable Tannin Improves Nonspecific Immune Responses, Intestinal Morphology, and Disease Resistance against Vibrio alginolyticus in Whiteleg Shrimp

Huang,

Hu,

Liao

et al. 2024

Aquaculture Research

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This research aimed to evaluate the impact of hydrolyzable tannin on immune responses, intestinal morphology, and resistance to Vibrio alginolyticus in whiteleg shrimp (Penaeus vannamei). In preliminary in vitro experiments, shrimp hemocytes were exposed to different concentrations of hydrolyzable tannin. The findings indicated that hydrolyzable tannin exhibited nontoxic characteristics and could stimulate respiratory burst activity. For the subsequent in vivo trial, shrimp (11.83 ± 0.98 g) were subjected to a 28-day oral administration of diets containing diverse hydrolyzable tannin concentrations (0, 1, 2, 4, and 8 g/kg denoted as control, T1, T2, T4, and T8, respectively). Immune parameters, antimicrobial peptide genes, and intestinal morphology were measured. Significant enhancements were observed in total hemocyte count (THC), phagocytic activity, respiratory burst activity, and phenoloxidase activity (PO) across all treatment groups compared to the control group, particularly in T2 and T4 groups (p<0.05). The gene expressions of Penaeidin-2, Penaeidin-3, Penaeidin-4, crustin, and lysozyme were markedly higher in the treatment groups, especially in T2 (p<0.05). Additionally, hydrolyzable tannin contributed to increased intestinal wall thickness after the 28-day feeding trial (p <0.05). In a separate challenge experiment, shrimps (11.36 ± 0.66 g) were exposed to V. alginolyticus after 7 days of culture. Notably, T2 and T4 exhibited enhanced resistance, resulting in survival rates of 66.67% and 69.70%, respectively. To conclude, administering 4 g of hydrolyzable tannin per kilogram of feed proved effective in enhancing nonspecific immune responses, improving intestinal morphology, and augmenting resistance to V. alginolyticus in whiteleg shrimp.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation with Hydrolyzable Tannin Improves Nonspecific Immune Responses, Intestinal Morphology, and Disease Resistance against Vibrio alginolyticus in Whiteleg Shrimp

Huang,

Hu,

Liao

et al. 2024

Aquaculture Research

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“…Microorganisms can exhibit both attraction and antagonism toward each other, depending on various factors such as their ecological niche, competition for resources, and the specific microorganisms involved [ 64 ]. The antagonistic interactions among microorganisms are considered a promising alternative approach for treating bacterial infections and are being explored for applications in aquaculture or disease prevention in humans [ 65 , 66 , 67 ]. Numerous studies are underway to investigate the mechanisms behind antagonistic interactions, with a growing emphasis on characterizing bacteriocins.…”

Section: Discussionmentioning

confidence: 99%

Safety Assessment and Probiotic Potential Comparison of Bifidobacterium longum subsp. infantis BLI-02, Lactobacillus plantarum LPL28, Lactobacillus acidophilus TYCA06, and Lactobacillus paracasei ET-66

Chen,

Hsia,

Kuo

et al. 2023

Nutrients

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Bifidobacterium longum subsp. infantis BLI-02, Lactobacillus paracasei ET-66, Lactobacillus plantarum LPL28, and Lactobacillus acidophilus TYCA06, isolated from healthy breast milk, miso, and the healthy human gut, were assessed for safety in this study. BLI-02, LPL28, TYCA06, and ET-66 exhibited no antibiotic resistance and mutagenic activity in the Ames test at the highest dosage (5000 μg/plate). No genotoxicity was observed in micronucleus and chromosomal aberration assays in rodent spermatogonia at the maximum dosage of 10 g/kg body weight (BW). No acute and sub-chronic toxicity occurred in mice and rats at the maximum tested dosage of 10 g/kg BW and 1.5 g/kg BW, respectively. The lyophilized powder of these strains survived a low pH and high bile salt environment, adhering strongly to Caco-2 cells. Unique antimicrobial activities were noted in these strains, with BLI-02 demonstrating the best growth inhibition against Vibrio parahaemolyticus, LPL28 exhibiting the best growth inhibition against Helicobacter pylori, and ET-66 showing the best growth inhibition against Aggregatibacter actinomycetemcomitans. Based on the present study, the lyophilized powder of these four strains appears to be a safe probiotic supplement at tested dosages. It should be applicable for clinical or healthcare applications.

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“…plantarum strains against Gram-negative ichthyopathogens, such as A. salmonicida [56], A. hydrophila [57], and Vibrio harveyi [58] Similarly, Lc. paracasei strains have previously been associated with probiotic effects in several farmed aquatic species, such as Nile tilapia [59], and white-leg shrimp (Penaeus vannamei) [60], as well as by demonstrating antimicrobial activity against Gram-negative aquatic pathogens such as Vibrio parahaemolyticus [61]. Moreover, the use of Lc.…”

Section: St Parauberis Lmg225mentioning

confidence: 99%

Antimicrobial Activity, Genetic Relatedness, and Safety Assessment of Potential Probiotic Lactic Acid Bacteria Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis) Used as Live Feed in Fish Larviculture

Contente,

Díaz-Formoso,

Feito

et al. 2024

Animals

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Aquaculture is a rapidly expanding agri-food industry that faces substantial economic losses due to infectious disease outbreaks, such as bacterial infections. These outbreaks cause disruptions and high mortalities at various stages of the rearing process, especially in the larval stages. Probiotic bacteria are emerging as promising and sustainable alternative or complementary strategies to vaccination and the use of antibiotics in aquaculture. In this study, potential probiotic candidates for larviculture were isolated from a rotifer-rearing tank used as the first live feed for turbot larvae. Two Lacticaseibacillus paracasei and two Lactiplantibacillus plantarum isolates were selected for further characterization due to their wide and strong antimicrobial activity against several ichthyopathogens, both Gram-positive and Gram-negative. An extensive in vitro safety assessment of these four isolates revealed the absence of harmful traits, such as acquired antimicrobial resistance and other virulence factors (i.e., hemolytic and gelatinase activities, bile salt deconjugation, and mucin degradation, as well as PCR detection of biogenic amine production). Moreover, Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) analyses unveiled their genetic relatedness, revealing two divergent clusters within each species. To our knowledge, this work reports for the first time the isolation and characterization of Lactic Acid Bacteria (LAB) with potential use as probiotics in aquaculture from rotifer-rearing tanks, which have the potential to optimize turbot larviculture and to introduce novel microbial management approaches for a sustainable aquaculture.

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Dietary of Lactobacillus paracasei and Bifidobacterium longum improve nonspecific immune responses, growth performance, and resistance against Vibrio parahaemolyticus in Penaeus vannamei

Cited by 20 publications

References 30 publications

Dietary Supplementation with Hydrolyzable Tannin Improves Nonspecific Immune Responses, Intestinal Morphology, and Disease Resistance against Vibrio alginolyticus in Whiteleg Shrimp

Dietary Supplementation with Hydrolyzable Tannin Improves Nonspecific Immune Responses, Intestinal Morphology, and Disease Resistance against Vibrio alginolyticus in Whiteleg Shrimp

Safety Assessment and Probiotic Potential Comparison of Bifidobacterium longum subsp. infantis BLI-02, Lactobacillus plantarum LPL28, Lactobacillus acidophilus TYCA06, and Lactobacillus paracasei ET-66

Antimicrobial Activity, Genetic Relatedness, and Safety Assessment of Potential Probiotic Lactic Acid Bacteria Isolated from a Rearing Tank of Rotifers (Brachionus plicatilis) Used as Live Feed in Fish Larviculture

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