Dietary nucleotides extend the life span in Sprague-Dawley rats

Xu, Mingzhen; Liang, Rui; Guo, Qingping; Wang, Shengkai; Zhao, Ming; Zhang, Z.; Wang, Junbo; Li, Yong

doi:10.1007/s12603-012-0399-z

Cited by 26 publications

(30 citation statements)

References 32 publications

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“…MDA is one of the degradation products of lipid peroxidation, an important indicator for evaluating cellular oxidative stress [25]. Studies indicated that NTs exhibit remarkable anti-oxidative activities [11,13]. Our results suggested that the anti-fatigue effects of NTs are closely related to the protection of corpuscular membrane by improving the activities of several enzymes and preventing lipid oxidation.…”

Section: Discussionmentioning

confidence: 57%

“…A number of reports have demonstrated that adding NTs to dietary formulas increases the production of immunoglobulins, improves response to vaccines, reduces morbidity, and increases tolerance to dietary antigens [12,14]. Our previous studies found that NTs are not toxic or carcinogenic in rats at a concentration of up to 0.64% (of body weight) for their entire lifetime, and could extend the lifespan in SD rats in a dose-dependent manner [13]. To the best of our knowledge, the present study is the first to report that dietary NTs supplementation improves the fatigue.…”

Section: Discussionmentioning

confidence: 99%

“…NTs have many beneficial functions, including antitumor acitivity, immune modulation, liver protective ability, and normalization of metabolism [9–12]. In addition, NTs exhibit superior antioxidant and anti-aging properties, as confirmed by our previous study [13]. However, studies on the anti-fatigue effects of NTs have rarely been reported.…”

Section: Introductionmentioning

confidence: 87%

“…Mice in the three experimental groups were fed with 0.01%, 0.16%, or 0.64% (wt/wt) NTs in the diet, respectively. The doses refer to the previous study in our laboratory [11–13]. Experimental mice were administrated by gavage for 30 days, and then were used for further experiments.…”

Section: Methodsmentioning

confidence: 99%

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Anti-fatigue effects of dietary nucleotides in mice

Liang

et al. 2017

Food & Nutrition Research

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As the building blocks of nucleic acids, nucleotides are conditionally essential nutrients that exhibit multifaceted activities. The present study aimed to evaluate the anti-fatigue effects of dietary nucleotides (NTs) on mice and explore the possible underlying mechanism. Mice were randomly divided into four experimental sets to detect different indicators. Each set of mice was then divided into four groups: (i) one control group and (ii) three NTs groups, which were fed diets supplemented with NTs at concentrations of 0%, 0.04%, 0.16%, and 0.64% (wt/wt). NTs could significantly increase the forced swimming time, enhance lactate dehydrogenase activity and hepatic glycogen levels, as well as delay the accumulation of blood urea nitrogen and blood lactic acid in mice after 30 days of treatment. NTs also markedly improved fatigue-induced alterations in oxidative stress biomarkers and antioxidant enzymes. Notably, NTs increased the mitochondrial energy metabolic enzyme activities in the skeletal muscles of mice. These results suggest that NTs exert anti-fatigue effects, which may be attributed to the inhibition of oxidative stress and the improvement of mitochondrial function in skeletal muscles. NTs could be used as a novel natural agent for relieving exercise fatigue.Abbreviations: ATP: adenosine triphosphate; BLA: blood lactic acid; GSH-Px: glutathione peroxidase; LDH: lactate dehydrogenase; MDA: malondialdehyde; NTs: dietary nucleotides; SDH: succinate dehydrogenase; SOD: superoxide dismutase; BUN: blood urea nitrogen

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

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Anti-fatigue effects of dietary nucleotides in mice

Liang

et al. 2017

Food & Nutrition Research

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“…Nucleoprotein may also have antioxidant capacity since dietary nucleotide, a component of nucleoprotein, has been shown the activating antioxidant enzymes in serum of rat (Xu et al. ).…”

Section: Introductionmentioning

confidence: 99%

Preventive effects of nucleoprotein supplementation combined with intermittent loading on capillary regression induced by hindlimb unloading in rat soleus muscle

et al. 2017

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Physical inactivity leads to muscle atrophy and capillary regression in the skeletal muscle. Intermittent loading during hindlimb unloading attenuates the muscle atrophy, meanwhile the capillary regression in the skeletal muscle is not suppressed. Nucleoprotein has antioxidant capacity and may prevent capillary regression. Therefore, we assessed the combined effects of intermittent loading with nucleoprotein supplementation on capillary regression induced by hindlimb unloading. Five groups of rats were assigned: control (CON), 7 days hindlimb unloading (HU), HU plus nucleoprotein supplementation (HU + NP), intermittent loading during HU (HU + IL), and intermittent loading combined with nucleoprotein supplementation during HU (HU + IL + NP). Seven days HU resulted in decrease in capillary number‐to‐fiber number (C/F) ratio accompanied with disuse‐associated changes in fetal liver kinase‐1 (Flk‐1), a proangiogenesis factor, and thrombospondin‐1 (TSP‐1), an antiangiogenesis factor, in the soleus muscle. In addition, citrate synthase (CS) activity was decreased and protein level of superoxide dismutase (SOD)‐2 was increased. Neither nucleoprotein supplementation nor intermittent loading prevented the decrease in the C/F ratio, whereas nucleoprotein supplementation combined with intermittent loading prevented the regression of capillary during unloading. Moreover, the levels of Flk‐1, TSP‐1, and SOD‐2 protein and the CS activity were maintained up to control levels. These results suggested that nucleoprotein supplementation combined with intermittent loading was effective to prevent capillary regression induced by muscle atrophy.

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