Dietary NaCl affects bleomycin-induced lung fibrosis in mice

Chen, Wensheng; Pilling, Darrell

doi:10.1080/01902148.2017.1385110

Cited by 8 publications

(7 citation statements)

References 72 publications

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“…This effect could not be seen in SD rats due to the aforementioned limitations of our study design; however, increased levels of total CD11b/c in granulocytes and CD3+ lymphocytes, together with the slightly increased frequency of CD11b/c-bearing lymphocyte subpopulations, support the conclusion that the HS diet activates PBL leading to leukocyte adhesion and migration, as well other effector mechanisms involved in inflammation [51]. For example, in the model of bleomycin-induced lung fibrosis, it has been shown that the LS diet normalizes leukocyte numbers and decreases CD11b- and CD11c-expressing cells in the lungs of mice [52]. Contrary to our findings in humans, the aHS group of rats presented with increased CD11a expression in PBL, but similarly to the human study, the frequency of certain highly CD11a-positive cells was decreased, suggesting their transmigration to the sites of inflammation.…”

Section: Discussionmentioning

confidence: 72%

Short-Term High-NaCl Dietary Intake Changes Leukocyte Expression of VLA-4, LFA-1, and Mac-1 Integrins in Both Healthy Humans and Sprague-Dawley Rats: A Comparative Study

Mihalj

Matić

Mihaljević

et al. 2019

Mediators of Inflammation

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This study is aimed at assessing the effects of a short-term high-salt (HS) diet on the peripheral blood leukocyte (PBL) activation status in healthy rats and young human individuals. Distribution of PBL subpopulations and surface expression of integrins were determined using flow cytometry in 36 men and women on a 7-day low-salt diet (<3.2 g salt/day) immediately followed by a 7-day HS diet (~14 g salt/day) or in Sprague-Dawley (SD) rats (n=24) on a 0.4% NaCl diet (aLS group) or a 4% NaCl diet (aHS group) for 7 days. The aHS group presented with an increased frequency of granulocytes, while the frequency of lymphocytes was reduced. Although in humans HS diet reduced the expression of CD11b(act) integrin on lymphocytes, the frequency of CD11b(act)-bearing cells among all PBL subsets was increased. The aHS group of rats exhibited increased expression of total CD11b/c in granulocytes and CD3 lymphocytes. The expression of CD11a was significantly reduced in all PBL subsets from human subjects and increased in the aHS group. CD49d expression on all PBL subsets was significantly decreased in both humans and rats. In human subjects, we found reduced frequencies of intermediate monocytes accompanied by a reciprocal increase in classical monocytes. Present results suggest that a short-term HS diet can alter leukocytes’ activation status and promote vascular low-grade inflammation.

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Section: Discussionmentioning

confidence: 72%

Short-Term High-NaCl Dietary Intake Changes Leukocyte Expression of VLA-4, LFA-1, and Mac-1 Integrins in Both Healthy Humans and Sprague-Dawley Rats: A Comparative Study

Mihalj

Matić

Mihaljević

et al. 2019

Mediators of Inflammation

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“…High-salt intake induces a chronic low-grade inflammation promoting not only hypertension (12), kidney damage (13), and cardiovascular disease (14) but also a more severe outcome in a number of inflammatory diseases (15)(16)(17)(18). This association appears to be related, at least in part, to the ability of sodium to modulate the function of either innate and adaptive immune cells.…”

Section: Introductionmentioning

confidence: 99%

High Salt Induces a Delayed Activation of Human Neutrophils

et al. 2022

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High salt (NaCl) concentrations are found in a number of tissues under physiological and pathological conditions. Here, we analyzed the effects induced by high salt on the function of human neutrophils. The culture of neutrophils in medium supplemented with high salt (50 mM NaCl) for short periods (30-120 min) inhibited the ability of conventional agonists to induce the production of IL-8 and the activation of respiratory burst. By contrast, exposure to high salt for longer periods (6-18 h) resulted in the activation of neutrophils revealed by the production of high levels of IL-8, the activation of the respiratory burst, and a marked synergistic effect on the production of TNF-α induced by LPS. Increasing osmolarity of the culture medium by the addition of sorbitol or mannitol (100 mM) was shown to be completely unable to stimulate neutrophil responses, suggesting that high sodium but not an increased osmolarity mediates the activation on neutrophils responses. A similar biphasic effect was observed when the function of monocytes was analyzed. Short term exposure to high salt suppressed IL-8 and TNF-α production induced by LPS while culture for longer periods triggered the production of IL-8 but not TNF-α in the absence of LPS stimulation. Contradictory results have been published regarding how high salt modulates neutrophil function. Our results suggest that the modulation of neutrophil function by high salt is strongly dependent on the exposure time.

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“…There is thus an urgent need for identification of dietary targets to prevent or limit intestinal fibrosis. Indeed, Western diet promotes fibrosis in studies from other organs 17 , 18 . Western diet is characterized by an insufficient intake of healthy foodstuffs and an excessive amount of saturated fats, sugar and salt.…”

Section: Discussionmentioning

confidence: 99%

Dietary salt exacerbates intestinal fibrosis in chronic TNBS colitis via fibroblasts activation

Amamou

Rouland

Yaker

et al. 2021

Sci Rep

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Intestinal fibrosis is a frequent complication in inflammatory bowel diseases (IBD). It is a challenge to identify environmental factors such as diet that may be driving this risk. Intestinal fibrosis result from accumulation of extracellular matrix (ECM) proteins secreted by myofibroblasts. Factors promoting intestinal fibrosis are unknown, but diet appears to be a critical component in its development. Consumption of salt above nutritional recommendations can exacerbate chronic inflammation. So far, high salt diet (HSD) have not been thoroughly investigated in the context of intestinal fibrosis associated to IBD. In the present study, we analyze the role of dietary salt in TNBS chronic colitis induced in rat, an intestinal fibrosis model, or in human colon fibroblast cells. Here, we have shown that high-salt diet exacerbates undernutrition and promoted ECM-associated proteins in fibroblasts. Taken together, our results suggested that dietary salt can activate intestinal fibroblasts, thereby contributing to exacerbation of intestinal fibrosis. Dietary salt may be considered as a putative environmental factor that drives intestinal fibrosis risk.

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Dietary NaCl affects bleomycin-induced lung fibrosis in mice

Cited by 8 publications

References 72 publications

Short-Term High-NaCl Dietary Intake Changes Leukocyte Expression of VLA-4, LFA-1, and Mac-1 Integrins in Both Healthy Humans and Sprague-Dawley Rats: A Comparative Study

Short-Term High-NaCl Dietary Intake Changes Leukocyte Expression of VLA-4, LFA-1, and Mac-1 Integrins in Both Healthy Humans and Sprague-Dawley Rats: A Comparative Study

High Salt Induces a Delayed Activation of Human Neutrophils

Dietary salt exacerbates intestinal fibrosis in chronic TNBS colitis via fibroblasts activation

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