Dietary myo-inositol deficiency decreased intestinal immune function related to NF-κB and TOR signaling in the intestine of young grass carp (Ctenopharyngodon idella)

Li, Shuang-An; Jiang, Wei‐Dan; Feng, Lin; Liu, Yang; Wu, Pei; Jiang, Jun; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Zhang, Yongan; Yang, Juan; Tang, Xu; Shi, He-Qun; Zhou, Xiao‐Qiu

doi:10.1016/j.fsi.2018.03.017

Cited by 42 publications

(15 citation statements)

References 70 publications

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“…In the present study, the serum TP and haemocyanin contents increased with increasing dietary MI levels and the highest serum AKP activity was found in those crabs fed 1,650 mg/kg MI. Various studies have shown that MI is an important substance for improving immunity of aquatic animals (Chen et al., 2018; Li et al., 2018). It was reported that dietary MI deficiency might break intestinal immune function through the NF‐κB and TOR pathway in grass carp ( Ctenopharyngodon idella ) (Li et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Effects ofmyo‐inositol on growth performance, body composition, antioxidant status, non‐specific immunity and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)

Lin

Liu

et al. 2020

Aquacult. Nutr.

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This study evaluated the effects of dietary myo‐inositol (MI) levels on growth, body composition, antioxidant status, non‐specific immunity and lipid metabolism in juvenile Chinese mitten crab (Eriocheir sinensis). A total of 960 crabs was fed with six diets supplemented with graded levels of MI (0, 204, 406, 810, 1,650 and 3,211 mg/kg) for 8 weeks. The crabs fed with diets containing 1,650 mg/kg MI had significantly higher final body weight, weight gain and specific growth rate than those fed 0, 204, 406 and 810 mg/kg MI (p < .05). The lowest moulting frequency was observed in the crabs fed diets without supplemented MI. The crabs fed with diets containing 1,650 and 3,211 mg/kg MI had significantly lower total lipid content in the whole body than those fed diets without supplemented MI (p < .05). The myo‐inositol level in hepatopancreas increased with increasing dietary MI up to 1,650 mg/kg before reaching plateau. The crabs fed 1,650 mg/kg MI had significantly higher total antioxidant capacity and glutathione peroxidase activity in hepatopancreas than those fed diets without supplemented MI (p < .05). However, the crabs fed diets without supplemented MI had the highest level of malondialdehyde in hepatopancreas compared with those fed with MI supplementation. The highest serum alkaline phosphatase activity was also observed in crabs fed 1,650 mg/kg MI. Total protein and haemocyanin contents in serum were significantly increased with increasing dietary MI levels (p < .05). The triglyceride contents in hepatopancreas significantly decreased with increasing dietary MI levels (p < .05). Results of real‐time quantitative PCR revealed that dietary MI reduced hepatic triglyceride accumulation probably through inhibiting lipid synthesis‐related genes and promoting lipid export‐related genes at the transcriptional level. Broken‐line analysis of weight gain, specific growth rate and MI levels in hepatopancreas against dietary MI levels indicates that the optimal MI requirement for juvenile crabs ranged from 1,613 to 1,707 mg/kg. The dietary MI supplementation can improve growth performance, antioxidant capacity and non‐specific immunity and reduce hepatopancreatic triglyceride accumulation in juvenile crab.

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Section: Discussionmentioning

confidence: 99%

Effects ofmyo‐inositol on growth performance, body composition, antioxidant status, non‐specific immunity and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)

Lin

Liu

et al. 2020

Aquacult. Nutr.

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“…Inositol is also a precursor of various biofunctional compounds containing inositol phosphate and lipid [5][6]. Inositol has attracted the attention of the food and feed industry, mainly used as an additive for drink, milk powder for nutrition supplement, and animal feed to prevent disease [7][8]. As a drug adulterant, inositol has been proved to treat some diseases such as diabetes and fatty liver [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Efficient production of myo-inositol in Escherichia coli through metabolic engineering

You

Wang

Shi

et al. 2020

Preprint

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Background The biosynthesis of high value-added compounds through metabolically engineered strains has received widely attention in recent years. As an effective compound in pharmaceutical, cosmetic and food industry, myo-inositol (inositol) is mainly produced via a harsh set of chemical reactions from phytate. The proper distribution of carbon flux between cell growth and inositol production was a major challenge for constructing an efficient inositol-synthetic pathway. Recombinant E. coli strains have been constructed by metabolic engineering strategies to produce inositol, yet with a low yield. Therefore, construction of E. coli metabolically engineered strains with high stoichiometric yield will be attractive. Results In the present study, the recombinant E. coli strains with high stoichiometric yield (> 0.7 mol inositol/mol glucose) were obtained to efficiently synthesize inositol. Inositol was successfully biosynthesized after introducing two crucial enzymes, inositol-3-phosphate synthase (IPS) from Trypanosoma brucei , and inositol monophosphatase (IMP) from E. coli. Based on starting strains E. coli BW25113 (wild type) and SG104 ( ΔptsG::glk , ΔgalR::zglf , ΔpoxB::acs ), a series of engineered strains for inositol production were constructed by deleting the key genes pgi, pfkA or pykF . Furthermore, the plasmid expression systems of IPS and IMP were optimized, and the gene zwf was regulated to enhance stoichiometric yield. The highest stoichiometric yield (0.96 mol inositol/mol glucose) was achieved with the combined strain R15 of SG104, Δpgi , Δpgm , and RBSL5-zwf. Simultaneously, the engineered strain R04 reached high-density fermentation level in a 1-L fermenter by using glucose and glycerol as mixed carbon source. In the scale-up bioconversion in situ with R04, 0.82 mol inositol/mol glucose was produced by fed-batch within 23 h, corresponding to a titer of 106.3 g/L (590.5 mM). Conclusions The biosynthetic pathway of inositol from glucose in recombinant E. coli was optimized by metabolic engineering strategies. The metabolically engineered E. coli strains represent a promising method for future inositol production. This study provided an essential reference to obtain a suitable distribution of carbon flux between glycolysis pathway and product synthetic pathway.

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“…Inositol is also a precursor of various biofunctional compounds containing inositol phosphate and lipid [5][6]. Inositol has attracted the attention of the food and feed industries, where it is mainly used as an additive in drinks, in milk powder as a nutritional supplement, and in animal feed to prevent diseases [7][8]. Inositol has been proved to treat some diseases such as diabetes and fatty liver [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Efficient production of myo-inositol in Escherichia coli through metabolic engineering

You

Wang

Shi

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The biosynthesis of high value-added compounds using metabolically engineered strains has received wide attention in recent years. Myo-inositol (inositol), an important compound in the pharmaceutics, cosmetics and food industries, is usually produced from phytate via a harsh set of chemical reactions. Recombinant Escherichia coli strains have been constructed by metabolic engineering strategies to produce inositol, but with a low yield. The proper distribution of carbon flux between cell growth and inositol production is a major challenge for constructing an efficient inositol-synthesis pathway in bacteria. Construction of metabolically engineered E. coli strains with high stoichiometric yield of inositol is desirable.Results: In the present study, we designed an inositol-synthesis pathway from glucose with a theoretical stoichiometric yield of 1 mol inositol/mol glucose. Recombinant E. coli strains with high stoichiometric yield (>0.7 mol inositol/mol glucose) were obtained. Inositol was successfully biosynthesized after introducing two crucial enzymes: inositol-3-phosphate synthase (IPS) from Trypanosoma brucei, and inositol monophosphatase (IMP) from E. coli. Based on starting strains E. coli BW25113 (wild-type) and SG104 (ΔptsG::glk, ΔgalR::zglf, ΔpoxB::acs), a series of engineered strains for inositol production was constructed by deleting the key genes pgi, pfkA and pykF. Plasmid-based expression systems for IPS and IMP were optimized, and expression of the gene zwf was regulated to enhance the stoichiometric yield of inositol. The highest stoichiometric yield (0.96 mol inositol/mol glucose) was achieved from recombinant strain R15 (SG104, Δpgi, Δpgm, and RBSL5-zwf). Strain R04 (SG104 and Δpgi) reached high-density in a 1-L fermenter when using glucose and glycerol as a mixed carbon source. In scaled-up fed-batch bioconversion in situ using strain R04, 0.82 mol inositol/mol glucose was produced within 23 h, corresponding to a titer of 106.3 g/L (590.5 mM) inositol.Conclusions: The biosynthesis of inositol from glucose in recombinant E. coli was optimized by metabolic engineering strategies. The metabolically engineered E. coli strains represent a promising method for future inositol production. This study provides an essential reference to obtain a suitable distribution of carbon flux between glycolysis and inositol synthesis.

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Dietary myo-inositol deficiency decreased intestinal immune function related to NF-κB and TOR signaling in the intestine of young grass carp (Ctenopharyngodon idella)

Cited by 42 publications

References 70 publications

Effects ofmyo‐inositol on growth performance, body composition, antioxidant status, non‐specific immunity and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)

Effects ofmyo‐inositol on growth performance, body composition, antioxidant status, non‐specific immunity and lipid metabolism of juvenile Chinese mitten crab (Eriocheir sinensis)

Efficient production of myo-inositol in Escherichia coli through metabolic engineering

Efficient production of myo-inositol in Escherichia coli through metabolic engineering

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