Dietary methylmercury alters the proteome in Atlantic salmon (Salmo salar) kidney

Nøstbakken, Ole Jakob; Martín, Samuel; Cash, Phillip; Torstensen, Bente E.; Amlund, Heidi

doi:10.1016/j.aquatox.2011.08.017

Cited by 23 publications

(13 citation statements)

References 34 publications

(34 reference statements)

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“…The expression of metallothionein, as a potent antioxidant, was noted in the liver of Cyprinus carpio from a mercury-contaminated river [20]. Nostbakken et al investigated the proteomic analysis in the kidney of Atlantic salmon exposed to methylmercury for 8 weeks [10]. Glutathione peroxidase-2 was increased in abundance in the kidney of methylmercury-exposed fish.…”

Section: Introductionmentioning

confidence: 97%

“…Glutathione peroxidase-2 was increased in abundance in the kidney of methylmercury-exposed fish. The increased glutathione peroxidase observed in their study may be the kidney's transcriptional response to oxidative stress [10].…”

Section: Introductionmentioning

confidence: 98%

“…Methylmercury is readily absorbed and bioaccumulates in fish and other aquatic organisms and is more toxic to fish than the inorganic form, the 96 h LC 50 for CH 3 HgCl and for HgCl 2 being 24 and 275 μg L −1 Hg, respectively [6]. The toxic effects of methylmercury have been revealed in the neurological system, liver, gonads, kidney, and muscles [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

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Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C

Mozhdeganloo

Jafari

Koohi

et al. 2015

Biol Trace Elem Res

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The present study was undertaken to evaluate methylmercury-induced alterations in hepatic enzymes and oxidative stress markers in liver tissue of rainbow trout (Oncorhynchus mykiss) by using a perfusion method, and to explore possible protective effect of vitamin C against these alterations. Forty-eight fish were divided into six groups containing control, test, and amelioration groups. The liver of fish in the test groups were exposed to different doses of methylmercury, i.e., 0.6, 1.2, and 2.4 μg L(-1), respectively, for 120 min. In the amelioration group, liver was treated with vitamin C (17.2 μg L(-1)) along with high dose (2.4 μg L(-1)) of methylmercury. The results of the present study showed that exposure with 0.6, 1.2, and 2.4 μg L(-1) of methylmercury significantly increased (p < 0.05) hepatic enzyme activities (alanine transaminase (ALT), aspartate transaminase (AST), and Lactate dehydrogenase (LDH)) and malondialdehyde (MDA) level, as a marker of lipid peroxidation. On the other hand, the concentration of reduced glutathione (GSH) and total antioxidant capacity of the liver decreased (p < 0.05) in the methylmercury-exposed groups when compared to the control group. Pearson's correlation analysis revealed a positive correlation between MDA concentration and ALT, AST, and LDH activities in the methylmercury groups, suggesting that the enhanced lipid peroxidation may be linked to hepatic damage caused by methylmercury. Treatment with vitamin C in methylmercury-exposed group led to a significant decrease (p < 0.05) in MDA concentration and hepatic enzyme activities and significant increase (p < 0.05) in levels of GSH and total antioxidant capacity. The values of measured parameters in the methylmercury + vitamin C group were comparable to those of the control group. The results of the present study demonstrated that methylmercury exposure induces oxidative stress in the liver of rainbow trout and treatment with vitamin C can protect fish liver against this oxidative insult.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C

Mozhdeganloo

Jafari

Koohi

et al. 2015

Biol Trace Elem Res

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“…previously applied this approach to analyze effects of salinity stress on the renal proteome of dogfish shark (Squalus acanthias) [60]. Bony fish species for which the renal proteome has been analyzed include zebrafish (Brachidanio rerio) [61,62], Atlantic salmon [63][64][65], smallmouth bass (Micropterus dolomieu) [66], channel catfish (Ictalurus punctatus) [67,68], gilthead sea bream (Sparus aurata) [69], and puffer fish (Takifugu rubripes) [70]. Most of these previous studies on teleosts focus on the effects of pathogens or toxins on the proteome of the head kidney.…”

Section: Discussionmentioning

confidence: 99%

Population-specific renal proteomes of marine and freshwater three-spined sticklebacks

Kültz

Paguio

et al. 2016

Journal of Proteomics

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Quantitative proteomics was used to reveal biochemical differences in kidneys of marine and freshwater three-spined sticklebacks. More than 1500 unambiguous proteins were identified, 106 of which are robustly co-translationally modified. Amino-terminal acetylation sites for 94 and proline hydroxylation sites for 12 proteins, including 4 protein disulfide isomerases having the consensus motif APWCGHCK, were determined. More than 1500 proteins were quantified by LC-MS/MS yielding 120 proteins with consistent population-specific abundance differences. Twenty-five of these were selected for validation by data-independent acquisition (DIA) and spectral library based MS2 quantitation. A dense biochemical network was revealed, which promotes the synthesis of the organic osmolytes betaine, sorbitol, trimethylamine oxid (TMAO), and urea. It contains 33 of 49 proteins that are elevated in marine compared to freshwater sticklebacks, including the most highly elevated proteins (dimethylaniline monooxygenase, alanine-glyoxylate aminotransferase, glycine N-methyltransferase). Freshwater stickleback kidneys contain elevated levels of proteolytic, cytoskeletal, extracellular matrix, and calcium signaling proteins. Proteins that are most elevated in freshwater sticklebacks are ES1 protein homolog, apoptosis-associated speck-like protein containing a CARD and caspase 1. Protein-abundance network analysis demonstrates significantly higher levels of synchronized abundance control in marine sticklebacks. The significance of these findings for biochemical diversification of renal function in marine and FW sticklebacks is discussed.

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“…These works lead not only to a better understanding of the molecular mechanisms of pathogenesis as well as provide novel protein candidates for vaccines development. Toxicological studies related to evaluation of chemically induced protein expression response have also been reported in Atlantic salmon [42], rainbow trout [43], cod [44,45] and rare minnow (Gobiocypris rarus) that has been recently selected as a model for aquatic toxicological studies [46][47][48].…”

Section: Vertebratesmentioning

confidence: 98%

PROTEOMICS in aquaculture: Applications and trends

Rodrigues

Silva

Dias

et al. 2012

Journal of Proteomics

122

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Dietary methylmercury alters the proteome in Atlantic salmon (Salmo salar) kidney

Cited by 23 publications

References 34 publications

Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C

Methylmercury-Induced Oxidative Stress in Rainbow Trout (Oncorhynchus mykiss) Liver: Ameliorating Effect of Vitamin C

Population-specific renal proteomes of marine and freshwater three-spined sticklebacks

PROTEOMICS in aquaculture: Applications and trends

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