Dietary methionine restriction improves the gut microbiota and reduces intestinal permeability and inflammation in high-fat-fed mice

Yang, Yuhui; Zhang, Yuanhong; Xu, Yao; Luo, Tingyu; Ge, Yueting; Jiang, Yuge; Shi, Yonghui; Sun, Jin; Li, Guowei

doi:10.1039/c9fo00766k

Cited by 74 publications

(85 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SCFAs play an important role in maintaining the integrity of the intestinal epithelial barrier by regulating tight junction proteins. 29,30 As expected, we found that NLRP3 deficiency compensates for gut barrier destruction induced by AP, as revealed by increased expression of Claudin-1 and Occludin in NLRP3 KO mice with AP. Collectively, we speculate that the knockout of NLRP3 resists the gut microbial dysbiosis induced by AP, including the enrichment of beneficial bacterial and reduction of pathogenic bacterial, which partly contributes to the alleviation of systematic inflammation (Figure 8).…”

Section: Discussionsupporting

confidence: 83%

The interplay between the gut microbiota and NLRP3 activation affects the severity of acute pancreatitis in mice

et al. 2020

Gut Microbes

View full text Add to dashboard Cite

Early dysbiosis of the gut microbiota is associated with the severity of acute pancreatitis (AP), although the underlying mechanism is unclear. Here, we investigated the role of crosstalk between NLRP3 and the gut microbiota in the development of AP utilizing gut microbiota deficient mice, as well as NLRP3 knockout (KO) mouse models. Pancreatic damage and systemic inflammation were improved in antibiotic-treated (Abx) and germ-free (GF) mice, accompanied by weakened activity of the intestinal NLRP3 inflammasome. Interestingly, fecal microbiota transplantation (FMT) reactivated the intestinal NLRP3 inflammasome and exacerbated the disease in Abx and GF mice. Although the gut barrier in GF and Abx mice was disrupted, gut microbiota deficiency ameliorated the severity of AP, probably due to the reduction in bacterial translocation from the gut to the pancreas. The composition of the gut microbiota was significantly different between NLRP3 KO mice and wild-type (WT) mice at baseline, and there were alterations in response to the induction of AP. While a dramatic shift in the gut microbiota with overgrowth of Escherichia-Shigella was observed in WT mice suffering from AP, there was no significant change in NLRP3 KO mice with or without AP, suggesting that NLRP3 deficiency counteracts AP-induced microbial disturbance. With a strengthened gut barrier and decreased systemic inflammation, NLRP3 KO mice showed less severe AP, as revealed by reduced pancreatic neutrophilic infiltration and necrosis. Taken together, these results identified the bidirectional modulation between the gut microbiota and NLRP3 in the progression of AP, which suggests the interplay of the host and microbiome during AP.

show abstract

Section: Discussionsupporting

confidence: 83%

The interplay between the gut microbiota and NLRP3 activation affects the severity of acute pancreatitis in mice

et al. 2020

Gut Microbes

View full text Add to dashboard Cite

show abstract

“…Consistent with these results, methionine biosynthesis was decreased in the post-Kasai group. Previous research has demonstrated that dietary methionine restriction improves the gut microbiota and reduces intestinal permeability and inflammation ( 27 ). We concluded that the gut microbiota, intestinal permeability, and inflammation were improved in the post-Kasai group.…”

Section: Discussionmentioning

confidence: 99%

Effects of Previous Kasai Surgery on Gut Microbiota and Bile Acid in Biliary Atresia With End-Stage Liver Disease

2021

View full text Add to dashboard Cite

Background and Aims: Biliary atresia (BA) is the most common cholestatic liver disease in neonates. Although the Kasai procedure can improve temporary biliary drainage in some cases, complications and liver fibrosis still develop. Liver transplantation is the ultimate treatment. The current study aimed to investigate the effect of previous Kasai surgery on gut microbiota and bile acid in BA with end-stage liver disease.Methods: Patients with BA with end-stage liver disease were divided into two groups according to whether they had previously undergone Kasai surgery (non-Kasai: n = 8, post-Kasai: n = 8). Metagenomic sequencing and ultraperformance liquid chromatography/tandem mass spectrometry were performed to identify the gut microbiota and bile acid.Results: Previous Kasai surgery had some effects on gut microbiota and bile acid in BA with end-stage liver disease. In the gut microbiome, the differential species were mainly distributed at the species level. Veillonella atypica had a significant increase in the non-Kasai group (P < 0.05). Bacteroides spp., Prevotella spp., Barnesiella spp., Parabacteroides spp., Heliobacterium spp., Erysipelatoclostridium spp. and Diaporthe spp. were increased in the post-Kasai group (P < 0.05). Concerning functional profiles, methionine biosynthesis was enriched in the non-Kasai group, while pyridoxal biosynthesis and riboflavin biosynthesis were enriched in the post-Kasai group (linear discriminant analysis > 2, P < 0.05). In stools, 17 bile acids were distinctly elevated in the post-Kasai group, such as cholic acid, chenodeoxycholic acid, β-muricholic acid and tauro α-muricholate (P < 0.05). Spearman correlation test showed that V. atypica had an enormously positive correlation with liver enzymes. Faecalibacterium prausnitzii and Escherichia coli were associated with derivatives of the alternative pathway of bile acid metabolism.Conclusion: Previous Kasai surgery can improve the gut microbiota and bile acid in patients with BA with end-stage liver disease. This improvement contributes to maintaining the intestinal barrier.

show abstract

“…Using chronic social defeat in male mice, we showed that some of the long-lasting bacterial changes in cecum contents were linked to severity of social avoidance (a behavioral feature reflective of susceptibility to the depressive-like effects of the stressor) and to brain pro-inflammatory cytokines ( Szyszkowicz et al., 2017 ). Although these observations were correlational, considering that the genera affected by the social stressor and correlated to behavioral and brain cytokine phenotypes ( Oscillospira and Parabacteroides ) have anti-inflammatory properties ( Koh et al., 2018 ; Yang et al., 2019 ), they suggest that severity of social avoidance in chronically defeated mice could be linked to an increased inflammatory tone along the gut microbiota-immune-brain axis. We confirmed this possibility by showing that male mice exposed to chronic social defeat had long-lasting elevations of the pro-inflammatory cytokines IL-1β and TNF-α in the middle section of the small intestine (jejunum), which were accompanied by abnormal expression of tight junction proteins (reflective of increased membrane permeability) ( Santos et al., 2021 ).…”

Section: The Gut Microbiota-immune-brain Axis and Depressive Illnessesmentioning

confidence: 94%

Beyond the neuro-immune interplay in depression: Could gut microbes be the missing link?

Audet

2021

Brain, Behavior, & Immunity - Health

View full text Add to dashboard Cite

Accumulating evidence have positioned inflammatory signaling pathways as crucial routes by which microbes inhabiting the gastrointestinal tract (the gut microbiota) communicate with the host brain to influence behavior, with impacts on mental illnesses. In this short review, an overview of inflammatory and gut microbiota status in human depression and in rodent models of the illness are provided. Next, potential inflammatory pathways mediating the communications between the gut and the brain under stressful conditions are described. Finally, dietary interventions targeting the gut microbiota-immune-brain axis in the context of depression are briefly discussed.

show abstract

Dietary methionine restriction improves the gut microbiota and reduces intestinal permeability and inflammation in high-fat-fed mice

Abstract: Dietary methionine restriction improved the intestinal microbiota composition, barrier function, oxidative stress, and inflammation in high-fat-fed mice.

Cited by 74 publications

References 68 publications

The interplay between the gut microbiota and NLRP3 activation affects the severity of acute pancreatitis in mice

The interplay between the gut microbiota and NLRP3 activation affects the severity of acute pancreatitis in mice

Effects of Previous Kasai Surgery on Gut Microbiota and Bile Acid in Biliary Atresia With End-Stage Liver Disease

Beyond the neuro-immune interplay in depression: Could gut microbes be the missing link?

Contact Info

Product

Resources

About