Dietary Mannan Oligosaccharides Modulate Gut Inflammatory Response and Improve Duodenal Villi Height in Post-Weaning Piglets Improving Feed Efficiency

Abstract

The aim of this study was to evaluate the effects of mannan oligosaccharides (MOS) on gut health and performance in post-weaning piglets. In total, 40 piglets were divided into two experimental groups and fed a basal diet with (TRT) or without (CON) 0.2% mannan oligosaccharides for 35 days. Growth performance was determined weekly and faecal microbial composition on days 0, 14 and 35. On day 36, histometrical evaluations were performed on duodenal, jejunal, ileal, and colon samples. mRNA gene expression of inflammation-related genes was evaluated in samples of ileal Peyer’s patches (IPP). MOS administration improved feed efficiency in the last two weeks of the trial (p < 0.05), and a decreased clostridia content was found in faeces at day 14 (p = 0.05). TRT piglets showed increased duodenal villi height (p < 0.05), and reduced mRNA levels of Tumour Necrosis Factor α (p < 0.05) and Toll-Like Receptor 4 (p < 0.01) in IPP. Our results suggest beneficial effects of MOS supplementation on gut morphology and the expression of inflammation-related genes in post-weaning piglets, accompanied by increased feed efficiency.

Manno-oligosaccharide attenuates inflammation and intestinal epithelium injury in weaned pigs upon enterotoxigenicEscherichia coliK88 challenge

“…The number of pigs used in each group (n 8) meets this minimal requirement of statistic for digestible trials or mechanism studies (e.g. six replicates are acceptable) (21,22) . Pigs received the same parental nutrition and management (e.g.…”

Selected feed additives used in pig nutrition

“…Similar effects were noted for piglets when the pregnant and lactating sows received inulin (Paßlack et al, 2015;McCormack et al, 2019;Li et al, 2020). Many studies Castillo et al, 2008;Agazzi et al, 2020;Zhou et al, 2020) focused on the effect of mannooligosaccharides (MOS) as an additive to feed for piglets at weaning age.…”

Amelioration of Enterotoxigenic Escherichia coli-induced disruption of intestinal epithelium by Manno-oligosaccharide in weaned pigs

“…For neonatal mammals including the pigs, disruption of the intestinal epithelium usually leads to altering intestinal permeability, facilitating the invasion of enteric pathogenic bacteria, and triggering intestinal in ammatory response [28][29][30]. MOS is a nondigestible oligosaccharide that has been reported to improve the growth performance and intestinal health due to its prebiotic effects [18,20]. In the present study, we investigated the potential of MOS against in ammation and disruption of the intestinal epithelium in weaned pigs exposure to ETEC.…”

“…Importantly, MOS was found to enhance the growth performance and attenuate the gastrointestinal in ammatory response in weaned pigs and other animal species [17][18][19]. Although, a number of studies indicated a bene cial effect of MOS on growth performance and gut health in animals [17,20,21], the mechanisms of action of MOS is just beginning to be explored.…”