Dietary influence on modulation of Helicoverpa armigera oral secretion composition leading to differential regulation of tomato plant defense

Kallure, Gopal S.; Shinde, Balkrishna A.; Barvkar, Vitthal T.; Kumari, Archana; Giri, Ashok P.

doi:10.1016/j.plantsci.2021.111120

Cited by 7 publications

(2 citation statements)

References 73 publications

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“…For ionization, dual electrospray ionization source was used. LC‐based metabolite separation, MS/MS fragmentation data acquisition, and metabolite peak area were calculated as per the method described (Kallure et al., 2022). Briefly, a Hypersil GOLD C18 column (2.1 × 150 mm, 1.9 μm particle size; Thermo Fisher Scientific, USA) at 40°C with a flow rate of 0.3 ml min −1 was used for LC‐based metabolite separation.…”

Section: Methodsmentioning

confidence: 99%

Ocimum kilimandscharicum4CL11 negatively regulates adventitious root development via accumulation of flavonoid glycosides

Lavhale,

Kondhare,

Sinthadurai

et al. 2024

The Plant Journal

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SUMMARY4‐Coumarate‐CoA Ligase (4CL) is an important enzyme in the phenylpropanoid biosynthesis pathway. Multiple 4CLs are identified in Ocimum species; however, their in planta functions remain enigmatic. In this study, we independently overexpressed three Ok4CL isoforms from Ocimum kilimandscharicum (Ok4CL7, ‐11, and ‐15) in Nicotiana benthamiana. Interestingly, Ok4CL11 overexpression (OE) caused a rootless or reduced root growth phenotype, whereas overexpression of Ok4CL15 produced normal adventitious root (AR) growth. Ok4CL11 overexpression in N. benthamiana resulted in upregulation of genes involved in flavonoid biosynthesis and associated glycosyltransferases accompanied by accumulation of specific flavonoid‐glycosides (kaempferol‐3‐rhamnoside, kaempferol‐3,7‐O‐bis‐alpha‐l‐rhamnoside [K3,7R], and quercetin‐3‐O‐rutinoside) that possibly reduced auxin levels in plants, and such effects were not seen for Ok4CL7 and ‐15. Docking analysis suggested that auxin transporters (PINs/LAXs) have higher binding affinity to these specific flavonoid‐glycosides, and thus could disrupt auxin transport/signaling, which cumulatively resulted in a rootless phenotype. Reduced auxin levels, increased K3,7R in the middle and basal stem sections, and grafting experiments (intra and inter‐species) indicated a disruption of auxin transport by K3,7R and its negative effect on AR development. Supplementation of flavonoids and the specific glycosides accumulated by Ok4CL11‐OE to the wild‐type N. benthamiana explants delayed the AR emergence and also inhibited AR growth. While overexpression of all three Ok4CLs increased lignin accumulation, flavonoids, and their specific glycosides were accumulated only in Ok4CL11‐OE lines. In summary, our study reveals unique indirect function of Ok4CL11 to increase specific flavonoids and their glycosides, which are negative regulators of root growth, likely involved in inhibition of auxin transport and signaling.

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Section: Methodsmentioning

confidence: 99%

Ocimum kilimandscharicum4CL11 negatively regulates adventitious root development via accumulation of flavonoid glycosides

Lavhale,

Kondhare,

Sinthadurai

et al. 2024

The Plant Journal

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show abstract

“…For ionization, dual electrospray ionization (ESI) source was used. LC-based metabolite separation, MS/MS fragmentation data acquisition, and metabolite peak area were calculated as per the method described 88 . The relative fold change of each metabolite was calculated by dividing the mass feature peak area of sample values against control values.…”

Section: Metabolite Profiling Of Wild-type and Transgenic Lines Of N ...mentioning

confidence: 99%

Ocimum kilimandscharicum 4CL11 negatively regulates root formation by inhibiting polar auxin transport via flavonoids accumulation

Lavhale

Kondhare

Sinthadurai

et al. 2023

Preprint

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4-Coumarate-CoA Ligase (4CL) is an important enzyme in the phenylpropanoid biosynthesis pathway. Multiple 4CLs are identified in Ocimum species; however, their function remains enigmatic. In this study, we independently overexpressed three Ok4CL isoforms from Ocimum kilimandscharicum (Ok4CL7, -11 and -15) in the plant species Nicotiana benthamiana, Solanum tuberosum and Arabidopsis thaliana for functional characterization. Interestingly, Ok4CL11 overexpression causes a rootless or reduced root growth phenotype, whereas overexpression of other two isoforms (Ok4CL7 and -15) resulted in normal adventitious root growth. Overexpression of Ok4CL11 in N. benthamiana leads to abundance of flavonoid-glycosides (i.e. kaempferol-3,7-O-bis-alpha-L-rhamnoside [K3,7R], quercetin-3-O-rutinoside [Q3R]), reduces auxin levels, and disrupts auxin transport and signaling, which cumulatively results in a rootless phenotype. In silico studies show that auxin transporters (PINs/LAXs) have higher binding affinity to K3,7R than to auxins, suggesting a potential disruption of auxin transport by the flavonoid-glycosides accumulated in Ok4CL11-OE lines. Our study reveals a unique indirect function of Ok4CL11 as a negative regulator of root growth, likely involving inhibition of auxin transport. As Ok4CL11 strongly affects root development, it is imperative to understand tissue-specific regulation of its gene expression and could be utilized for agricultural benefits.

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Induced defense responses in cultivated and wild chickpea genotypes against Helicoverpa armigera infestation

et al. 2023

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Dietary influence on modulation of Helicoverpa armigera oral secretion composition leading to differential regulation of tomato plant defense

Cited by 7 publications

References 73 publications

Ocimum kilimandscharicum4CL11 negatively regulates adventitious root development via accumulation of flavonoid glycosides

Ocimum kilimandscharicum4CL11 negatively regulates adventitious root development via accumulation of flavonoid glycosides

Ocimum kilimandscharicum 4CL11 negatively regulates root formation by inhibiting polar auxin transport via flavonoids accumulation

Induced defense responses in cultivated and wild chickpea genotypes against Helicoverpa armigera infestation

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