Dietary histidine requirement of Nile tilapia juveniles based on growth performance, expression of muscle-growth-related genes and haematological responses

Effects of dietary L‐lysine supplementation on growth, body composition and muscle‐growth‐related gene expression with an estimation of lysine requirement of GIFT tilapia

“…Conversely, the myostatin expression was not significantly affected ( p > .05) and downregulated by the treatments. Recently, it has been reported that the dietary supplementation of histidine significantly ( p < .05) affected the mRNA expression of MyoD and myogenin in the white skeletal muscle tissue of juvenile Nile tilapia, but did not affect the mRNA expression of myostatin (Michelato et al, ), which is in agreement with the present study where significant differences were observed in the relative expression of MyoD and myogenin and no significant differences were observed in the relative expression of myostatin . Our study shows that a dietary supplementation with synthetic amino acid L‐lysine is efficiently utilized by GIFT tilapia which was evident from the improved growth performances and feed utilization.…”

“…The blood parameter values obtained from the present study were in agreement with the findings of Badawi and Said (1971) (Rescan, Jutel, & Rallière, 2001;Watabe, 2000). Correspondingly, the present study observed that the relative expression of MyoD and myogenin was higher in fish fed lysine at 19 g/kg compared to fish fed lysine at 16 g/kg, which indicates that supplying the optimum level of lysine meeting the but did not affect the mRNA expression of myostatin (Michelato et al, 2017), which is in agreement with the present study where significant differences were observed in the relative expression of MyoD and myogenin and no significant differences were observed in the relative expression of myostatin. Our study shows that a dietary supplementation with synthetic amino acid L-lysine is efficiently utilized by GIFT tilapia which was evident from the improved growth performances and feed utilization.…”

“…However, values for Hb and PVC were similar to the values obtained in Heteropneustes fossilis fed graded concentrations of L‐lysine (Khan & Abidi, ). In contrast, no significant ( p > .05 ) changes were observed in the haematological and biochemical responses of Nile tilapia fed graded levels of dietary histidine‐ (Michelato et al, ) and arginine (Yue et al, )‐supplemented diets. However, Buentello, Reyes‐Becerril, Jesús Romero‐Geraldo, and Jesús Ascencio‐Valle () found that haemoglobin, haematocrit and erythrocyte values were influenced by high dietary arginine supplementation in channel catfish.…”

“…The first‐strand complementary DNA (cDNA) was synthesized from 2 μg of total RNA using the first‐strand cDNA synthesis kit (Thermo Scientific) following the manufacturer's protocol. The protocols of Michelato et al () were followed for relative gene expression studies (Table ). The quantitative real‐time‐polymerase chain reaction (qRT‐PCR) consisted of 20 ng of cDNA template, 10 μM of each primer (forward and reverse), and 1x SYBR Green PCR Master Mix Kit (Takara Bio Inc.), in a 20 μl of total volume.…”

“…The first-strand complementary DNA (cDNA) was synthesized from 2 μg of total RNA using the first-strand cDNA synthesis kit (Thermo Scientific) following the manufacturer's protocol. The protocols of Michelato et al (2017) were followed for relative gene expression studies ( Table 3) programmed with an initial denaturation at 95°C for 10 min, followed by 40 cycles of 15 s denaturation at 95°C, annealing at 60°C for 30 s, extension at 72°C for 30 s and ended with dissolution curve. The qRT-PCR was performed in triplicate; the threshold cycle (Ct) values obtained by amplification were measured; and a relative expression level of specific gene was presented as 2 −ΔΔCt (Livak & Schmittgen, 2001).…”