Dietary hemin promotes colonic preneoplastic lesions and DNA damage but not tumor development in a medium-term model of colon carcinogenesis in rats

Moura, Nelci Antunes de; Caetano, Brunno Felipe Ramos; Bidinotto, Lucas Tadeu; Rodrigues, Maria Aparecida Marchesan; Barbisan, Luı́s Fernando

doi:10.1016/j.mrgentox.2019.07.006

Cited by 2 publications

(1 citation statement)

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“…306-37-6) was used at a dose of 40 mg/kg of body weight (BW), diluted in aqueous solution of ethylenediamine tetra-acetic acid (EDTA—0.37 mg/mL), with pH correction to 6.5 by adding 0.1 N NAOH [ 18 ], intraperitoneally (ip), with modification by Limeiras et al [ 19 ], Navarro et al [ 14 ], Pamplona-Silva et al [ 11 ], and Pesarini et al [ 10 ]. Two doses were administered per week for two weeks [ 20 , 21 , 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

The Use of Natural Fiber-Rich Food Product Is Safe and Reduces Aberrant Crypt Foci in a Pre-Clinical Model

et al. 2021

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Background: Colorectal cancer is a highly prevalent disease, requiring effective strategies for prevention and treatment. The present research aimed to formulate a natural fiber-rich food product (NFRFP) and to evaluate its safety, toxicogenetics, and effects on aberrant crypt foci induced by 1,2-dimethyl-hydrazine in a preclinical model. Methods: A total of 78 male Wistar rats were distributed in six experimental groups: negative control, positive control (1,2-Dimethylhydrazine—40 mg/Kg), and four groups fed with 10% NFRFP: NFRFP, pre-treatment protocol, simultaneous treatment, and post-treatment protocol. Results: The NFRFP was shown to be a good source of fibers and did not change biometric, biochemical, hematological, and inflammatory parameters, and did not induce signs of toxicity and genotoxicity/carcinogenicity. NFRFP exhibited a chemopreventive effect, in all protocols, with damage reduction (% DR) of 75% in the comet test. NFRFP reduced the incidence of aberrant crypt outbreaks by 49.36% in the post-treatment protocol. Conclusions: The results suggest the applicability of NFRFP in the human diet due to potential production at an industrial scale and easy technological application in different products, since it could be incorporated in food without altering or causing small changes in final product sensory characteristics.

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