Dietary Grape Seed Meal Bioactive Compounds Alleviate Epithelial Dysfunctions and Attenuates Inflammation in Colon of DSS-Treated Piglets

Pistol, Gina Cecilia; Bulgaru, Cristina Valeria; Marin, Daniela Eliza; Oancea, Alexandra; Ţăranu, Ionelia

doi:10.3390/foods10030530

Cited by 17 publications

(23 citation statements)

References 78 publications

(68 reference statements)

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“…The effects produced by the experimental diets on genes encoding for tight junction markers Claudin 4 (CLDN4), Zonula1 (ZO1), and Occludin (OCCLDN) were determined by qPCR. Methods cited in Pistol et al, 2021 were used to extract total ARN, the transcription into complementary DNA (cDNA) and the quantitative PCR [ 84 ]. The Excel-based NormFinder software was utilised to normalise qPCR data and reference genes selected from 6 housekeeping genes analysed.…”

Section: Methodsmentioning

confidence: 99%

“…A Pierce BCA Protein Assay Kit, Thermo Fischer Scientific, USA, was used to quantify the protein content within the obtained lysates. A 10% SDS-PAGE electrophoresis was performed, and 30 µg of proteins were moved to the nitrocellulose membrane, blocked and incubated with specific primary and secondary antibodies as described by Pistol et al, 2021 [ 84 ]. The MicroChemi Imager (DNR Bio-Imaging Systems LTD, Neve Yamin, Israel) was used to develop the immunoblotting images, and the GelQuant software (DNR Bio-Imaging Systems LTD, Neve Yamin, Israel) was utilised to assay the protein expression level.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Grosu

Bulgaru

Pistol

et al. 2023

Toxins

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Pigs are the most sensitive animal to zearalenone (ZEN) contamination, especially after weaning, with acute deleterious effects on different health parameters. Although recommendations not to exceed 100 µg/kg in piglets feed exists (2006/576/EC), there are no clear regulations concerning the maximum limit in feed for piglets, which means that more investigations are necessary to establish a guidance value. Due to these reasons, the present study aims to investigate if ZEN, at a concentration lower than the EC recommendation for piglets, might affect the microbiota or induce changes in SCFA synthesis and can trigger modifications of nutritional, physiological, and immunological markers in the colon (intestinal integrity through junction protein analysis and local immunity through IgA production). Consequently, the effect of two concentrations of zearalenone were tested, one below the limit recommended by the EC (75 µg/kg) and a higher one (290 µg/kg) for comparison reasons. Although exposure to contaminated feed with 75 µg ZEN/kg feed did not significantly affect the observed parameters, the 290 µg/kg feed altered several microbiota population abundances and the secretory IgA levels. The obtained results contribute to a better understanding of the adverse effects that ZEN can have in the colon of young pigs in a dose-dependent manner.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Grosu

Bulgaru

Pistol

et al. 2023

Toxins

Self Cite

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“…Since 2016 ( 1 ), a limited number of new molecules have been tested in animal model studies of IBD ( Table 2 ). These include betulinic acid ( 57 ), goat whey ( 58 ), polyphenols ( 59 ), eriocitrin ( 60 ), proglitazone ( 61 ), dietary products from grape seeds ( 62 , 67 ) and lupin extracts ( 63 ) and traditional Chinese herbal preparations ( 64 ). In clinical studies, a cannabinoid receptor 2 agonist was found to improve mucosal healing ( 65 ).…”

Section: Therapeutic Implicationsmentioning

confidence: 99%

How to place the duality of specific MMP-9 inhibition for treatment of inflammatory bowel diseases into clinical opportunities?

2022

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Crohn’s disease (CD) and ulcerative colitis (UC) are inflammatory bowel diseases (IBD) with the involvement of immune cells and molecules, including cytokines, chemokines and proteases. A previous extensive review about the molecular biology of matrix metalloproteases (MMPs) and tissue inhibitors of metalloproteases (TIMPs), related to intestinal barrier destruction and restoration functions in IBD, is here complemented with the literature from the last five years. We also compare IBD as a prototypic mucosal inflammation of an epithelial barrier against microorganisms with inflammatory retinopathy as a disease with a barrier dysfunction at the level of blood vessels. Multiple reasons are at the basis of halting clinical trials with monoclonal antibodies against MMP-9 for IBD treatment. These include (i) the absence of a causative role of MMP-9 in the pathology in animal models of IBD, (ii) the fact that endotoxins, crossing the intestinal barrier, induce massive local release of both neutrophil collagenase (MMP-8) and gelatinase B (MMP-9), (iii) insufficient recognition that MMPs modify the activities of cytokines, chemokines and their receptors, (iv) ignorance that MMPs exist as mixtures of proteoforms with different posttranslational modifications and with different specific activities and (v) the fact that MMPs and TIMPs act in an interactive network, possibly having also beneficial effects on IBD evolution. Nevertheless, inhibition of MMPs may be a useful therapeutic approach during specific IBD disease phases or in specific sub-phenotypes. This temporary “window of opportunity” for MMP-9 inhibition may be complemented by a locoregional one, provided that the pharmacological agents are targeted in time to affected tissues, as is achieved in ophthalmological inflammation. Thus, in order to discover spatial and temporal windows of opportunity for MMP inhibition as treatment of IBD, more preclinical work including well controlled animal studies will be further needed. In this respect, MMP-9/NGAL complex analysis in various body compartments is helpful for better stratification of IBD patients who may benefit from anti-MMP-9.

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“…In this sense, natural bioactive substances such as plant polyphenols are emerging as sports nutrition supplements for GIS prevention ( 22 , 23 ). Research studies have recently indicated that a Mediterranean diet containing a high proportion of polyphenolic compounds ( 24 ) could significantly prevent intestinal barrier dysfunction ( 25 ). The phenolic compounds such as pterostilbene (PTE) are widely reported and rooted in blueberries and grapes ( 26 ).…”

Section: Introductionmentioning

confidence: 99%

Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise

et al. 2022

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Mounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE’s ability to prevent the loss of intestinal epithelial barrier in high loading intensity of exercise. The exercise model was established by the forced running of mice. An effective HPLC-UV method was developed to quantify PTE concentration in intestinal content. The mRNA changes were detected by quantitative polymerase chain reaction (qPCR). The structure of intestinal flora was analyzed by 16S rRNA sequencing. The PTE (100 mg/kg/d) could significantly attenuate exercise-induced intestinal epithelial barrier loss. Moreover, the HPLC-UV assay showed that the PTE concentration of intestinal content could last 12 h. Furthermore, the exercise increased the abundance of Alistipes, which was related to lipopolysaccharide (LPS) production but could not be reversed by PTE intervention. Besides, cell experiments showed that PTE could promote the expression of intestinal epithelial tight junction (TJ) molecules in vitro. In conclusion, PTE has a significant interest in preventing exercise-induced intestinal damage.

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Dietary Grape Seed Meal Bioactive Compounds Alleviate Epithelial Dysfunctions and Attenuates Inflammation in Colon of DSS-Treated Piglets

Cited by 17 publications

References 78 publications

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

Effects of Exposure to Low Zearalenone Concentrations Close to the EU Recommended Value on Weaned Piglets’ Colon

How to place the duality of specific MMP-9 inhibition for treatment of inflammatory bowel diseases into clinical opportunities?

Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise

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