“…However, the lower weight gain rate in the un‐supplemented group demonstrated the importance of dietary FA, in agreement with the results in hybrid tilapia (Shiau & Huang, ), grass carp fingerling, Ctenopharyngodon idella (Zhao et al., ) and grouper (Lin et al., ). Based on the weight gain rate, the optimum amount of FA supplementation for GIFT was 0.4 mg/kg, which is similar to the requirement of young rainbow trout (0.3–0.6 mg/kg) but less than that reported for juvenile grouper (0.8 mg/kg), grass shrimp P. monodon (1.9–2.1 mg/kg; Shiau & Huang, ) and juvenile abalone Haliotis discus hannai Ino (2.62–5.29 mg/kg; Miao, Zhang, Xu, & Mai, ); these findings showed that dietary FA requirements differed among the different species. However, when tilapia was the research objective, our results revealed that the dietary FA requirement of GIFT was 0.4–0.7 mg/kg based on the weight gain rate and liver FA concentration, 0.82 mg/kg for hybrid tilapia based on weight gain rate (Shiau & Huang, ), 0.5–1.0 mg/kg for Nile tilapia based on growth response and hematology (Lim & Klesius, ), the results on dietary FA requirements of tilapia differed.…”