Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

Berecki, Géza; Ruijter, Hester M. den; Verkerk, Arie O.; Schumacher, Cees A.; Baartscheer, Antonius; Bakker, Diane; Boukens, Bastiaan J.; Ginneken, A. C. G. Van; Fiolet, J.W.T.; Opthof, Tobias; Coronel, Ruben

doi:10.1016/j.hrthm.2007.07.015

Cited by 33 publications

(24 citation statements)

References 30 publications

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“…Furthermore, in cardiomyopathic hamsters, n-3 PUFA administration led to a significant improvement in the peak rate of tension rise and peak rate of tension fall, which indicated a faster rate of muscle contraction and relaxation, respectively (15). These effects are likely related to the prevention of Ca 2ϩ overload and to the decrease of sarcoplasmatic reticulum Ca 2ϩ content (16). Taken together, these findings may suggest potential mechanisms underlying the amelioration of diastolic function parameters observed in our active treatment group.…”

Section: Discussionmentioning

confidence: 95%

Effects of n-3 Polyunsaturated Fatty Acids on Left Ventricular Function and Functional Capacity in Patients With Dilated Cardiomyopathy

Nodari

Triggiani

Campia

et al. 2011

Journal of the American College of Cardiology

184

133

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Section: Discussionmentioning

confidence: 95%

Effects of n-3 Polyunsaturated Fatty Acids on Left Ventricular Function and Functional Capacity in Patients With Dilated Cardiomyopathy

Nodari

Triggiani

Campia

et al. 2011

Journal of the American College of Cardiology

184

133

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“…To observe arrhythmias (ventricular extrasystoles), the cells were bathed with 10 nM isoproterenol and stimulated with a 30-s pacing period (1.0 Hz), followed by a 30-s rest period [20,26]. Confocal imaging was performed using a Zeiss LSM510 confocal microscope (Carl Zeiss, Oberkochen, Germany) equipped with an argon laser (488 nm) and a 60 Â , 1.3 NA oil immersion objective set at axial and radial resolutions of 1.0 and 0.4 mm, respectively.…”

Section: Calcium Channelingmentioning

confidence: 99%

Nonenzymatic lipid mediators, neuroprostanes, exert the antiarrhythmic properties of docosahexaenoic acid

Roy

Oger

Thireau

et al. 2015

Free Radical Biology and Medicine

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Neuroprostanes are lipid mediators produced by nonenzymatic free radical peroxidation of docosahexaenoic acid (DHA). DHA is associated with a lower atherosclerosis risk, suggesting a beneficial role in cardiovascular diseases. The aim of this study was to investigate the influence of DHA peroxidation on its potentially antiarrhythmic properties (AAP) in isolated ventricular cardiomyocytes and in vivo in post-myocardial infarcted mice. Calcium imaging and biochemical experiments indicate that cardiac arrhythmias induced by isoproterenol are associated with Ca(2+) leak from the sarcoplasmic reticulum after oxidation and phosphorylation of the type 2 ryanodine receptor (RyR2) leading to dissociation of the FKBP12.6/RyR2 complex. Both oxidized DHA and 4(RS)-4-F4t-NeuroP prevented cellular arrhythmias and posttranslational modifications of the RyR2 leading to a stabilized FKBP12.6/RyR2 complex. DHA per se did not have AAP. The AAP of 4(RS)-4-F4t-NeuroP was also observed in vivo. In this study, we challenged the paradigm that spontaneously formed oxygenated metabolites of lipids are undesirable as they are unconditionally toxic. This study reveals that the lipid mediator 4(RS)-4-F4t-neuroprostane derived from nonenzymatic peroxidation of docosahexaenoic acid can counteract such deleterious effects through cardiac antiarrhythmic properties. Our findings demonstrate 4(RS)-4-F4t-NeuroP as a mediator of the cardioprotective AAP of DHA. This discovery opens new perspectives for products of nonenzymatic oxidized ω3 polyunsaturated fatty acids as potent mediators in diseases that involve ryanodine complex destabilization such as ischemic events.

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“…Panel (B) shows the average effect of the PV spontaneous activity generated by the EPA in the presence of L-NAME (n = 6). currents and sodium-calcium exchanger activity, while EPA increased inward rectifier potassium currents, and increased slow delayed rectifier potassium currents (Berecki et al, 2007;Saravanan et al, 2010;Verkerk et al, 2006). Decreased L-type calcium currents and decreased sodium-calcium exchanger activity may reduce the PV spontaneous activity and triggered activity (Wongcharoen et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Other reports indicate that acute administration of ω3-PUFAs docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) inhibits the formation of noradrenalin-induced triggered activity and reduces the number of delayed afterdepolarizations (DADs) and calcium aftertransients (Den Ruijter et al, 2008). Fish oil creates anti-arrhythmic potentials through ionic regulation (Berecki et al, 2007;Li et al, 2009;Verkerk et al, 2006). However, the anti-AF mechanism of EPA remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Eicosapentaenoic acid reduces the pulmonary vein arrhythmias through nitric oxide

et al. 2011

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Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

Cited by 33 publications

References 30 publications

Effects of n-3 Polyunsaturated Fatty Acids on Left Ventricular Function and Functional Capacity in Patients With Dilated Cardiomyopathy

Effects of n-3 Polyunsaturated Fatty Acids on Left Ventricular Function and Functional Capacity in Patients With Dilated Cardiomyopathy

Nonenzymatic lipid mediators, neuroprostanes, exert the antiarrhythmic properties of docosahexaenoic acid

Eicosapentaenoic acid reduces the pulmonary vein arrhythmias through nitric oxide

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