Dietary Fish Oil and Vitamin E Reduce Cryoinjuries and Apoptosis in Vitrified Laying Hens' Ovarian Tissue

Nateghi, Reihaneh; Masouleh, AliReza Alizadeh Moghadam; Ahangari, Y Jafari; Fathi, Rouhollah; Akhlaghi, A.

doi:10.1089/bio.2018.0101

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…Additionally, spermatozoa from asthenozoospermic patients have lower membrane DHA compared to normozoospermic individuals (Safarinejad, ) whereas sole FO enriched serum has no effective roles in cryopreserved human sperm parameters. More recently, this hypothesis was supported in laying hens, which noted that dietary fish oil in the presence of antioxidants improved the vitrified ovarian gene expression and histological parameters (Nateghi, Alizadeh Moghadam Masouleh, Jafari Ahangari, Fathi, & Akhlaghi, ).…”

Section: Discussionmentioning

confidence: 88%

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

et al. 2020

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The objective was to evaluate the effect of inclusion of 2.5% and 5% ovine serum, enriched with vitamin E (Vit E) and fish oil (FO), in human sperm freezing medium. Serum samples were prepared from sixteen rams (n = 4) feeding on a without supplemented diet, and diets supplemented with Vit E, FO and Vit E + FO. Semen samples, from 60 normozoospermic men, were frozen in: (I) a commercial freezing medium (SpermFreeze™; control medium), (II) the commercial freezing medium containing foetal bovine serum, (III) the commercial freezing medium + nonenriched serum (serum group), (IV) the commercial freezing medium + Vit E enriched serum (Vit E group), (V) the commercial freezing medium + FO enriched serum (FO group) and (VI) the commercial freezing medium + Vit E + FO enriched serum (Vit E + FO group). Sperm total and progressive motility, morphology, viability and plasma membrane integrity were significantly higher (p ≤ .05) in Vit E and Vit E + FO groups compared with the control group. Mitochondrial membrane potential did not differ between treatments (p > .05). It was concluded that ovine serum enriched with vitamin E and vitamin E + FO improved the quality of human spermatozoa but enriched serum containing FO could not improve the sperm cryo‐injuries.

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Section: Discussionmentioning

confidence: 88%

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

et al. 2020

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“…Our result is consistent with other vitrification studies in hen ovary [ 6 ], which successfully maintained follicle morphology with similar vitrification solutions except for the presence of chick serum in our study. The same group also reported the further improvement in follicle viability of hen ovarian tissues by supplementation of dietary polyunsaturated fatty acid and vitamin E as additional cryoprotectants [ 7 ]. A study in Japanese quail by another group showed that cell viability (but not follicle viability) in vitrified ovarian tissues were evaluated by trypan blue [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…The chicken has a single ovary with different-sized follicles, which enclose primary oocytes, including a primordial follicle, primary follicles, and a pre-hierarchical follicle stage. While cryopreservation of ovarian tissue has been studied in mammals as a potential method to preserve female fertility, including in women threatened by cancer therapies [ 5 ], it has been also attempted in hens [ 6 , 7 ] and Japanese quail [ 8 , 9 ]. Moreover, some studies have revealed that ovarian tissue cryopreservation can be of use for transplantation, resulting in successful birth, for genetic preservation purposes [ 8 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation Competence of Chicken Oocytes as a Model of Endangered Wild Birds: Effects of Storage Time and Temperature on the Ovarian Follicle Survival

Fujihara

Shiraishi

Onuma

et al. 2022

Animals

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For the conservation of endangered avian species, developing gamete preservation technologies is essential. However, studies in oocytes have not been widely conducted. In this study, assuming that the ovaries are transported to a research facility after death, we investigated the effect of ovary storage on oocytes for the purpose of cryopreserving avian female gametes by using a chicken as a model of endangered avian species. After excision, the ovaries were stored at either a low temperature (4 °C) or room temperature for 1–3 days. Ovarian follicles stored under different conditions for each period were examined by neutral red staining, histology, and gene and protein expression analysis. In addition, the pH of the storage medium after preserving the ovaries was measured. Then, ovarian tissues were vitrified to determine the cryopreservation competence. Storing the ovarian tissues at 4 °C kept the follicles viable and morphologically normal for 3 days with slow decline. In contrast, although different storage temperature did not influence follicle viability and morphology after only 1 day of storage, ovarian tissues stored at room temperature rapidly declined in structurally normal follicles, and viable follicles were rarely seen after 3 days of storage. Gene and protein expression analysis showed that apoptosis had already started on the first day, as shown by the higher expression of CASP9 under room temperature conditions. Furthermore, high expression of SOD1 and a rapid decline of pH in the storage medium under room temperature storage suggested the influence of oxidative stress associated with low pH in this condition on the follicle survivability in hen ovarian tissues. Our cryopreservation study also showed that ovarian tissues stored at 4 °C could recover after cryopreservation even after 3 days of storage. The described storage conditions and cryopreservation methods, which preserve chicken follicle survival, will lay the foundation of ovarian tissue preservation to preserve the fertility of wild female birds.

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“…Details of the primer design were presented by previous study (Nateghi et al, 2019) at Royan Institute, Tehran, Iran. Specific primers design for Bcl2 antagonist/killer (Bak), Bcl2 apoptosis regulator (Bcl2), caspase 3 (Cas3), caspase 8 (Cas8), caspase 9 (Cas9), fatty acid elongase 2 (Elovl2), fatty acid elongase 4 (Elovl4), fatty acid elongase 5 (Elovl5), fatty acid desaturase 1 (Fads1), fatty acid desaturase 2 (Fads2) and Stearoyl-COA desaturase (Scd), were done using the software Perlprimer.…”

Section: Sample Collection and Quantitative Real-time Pcr Proceduresmentioning

confidence: 99%

“…At 24 weeks of age, dietary LC supplementation markedly induced Bcl2, The characteristics of primers used for real-time PCR(Nateghi et al, 2019) Bak/Bcl2 ratio and Cas8 (p < .05).The expression of Bak and Bak/ Bcl2 ratio was linearly decreased by dietary LC (p < .05). Moreover, expression of Bcl2 and cas8 had quadratic responses to LC levels.…”

mentioning

confidence: 98%

Dietary L‐carnitine affects the expression of genes involved in apoptosis and fatty acid metabolism in rooster testes

et al. 2020

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Thirty‐six 12‐week‐old breeder roosters (Ross 308) were randomly allocated into three groups to receive L‐carnitine (LC): LC‐0, LC‐250 or LC‐500 mg/kg of diet to evaluate the effects of dietary LC on the expression of apoptotic‐related genes and desaturases and elongase mRNA transcript levels, in the cockerel testicles. Alteration of Bak (Bcl2 antagonist/killer), Bcl2, Cas3, Cas8, Cas9, Elovl2, Elovl4, Elovl5, Fads1, Fads2 and Scd expression at 24 and 34 weeks of age was compared by real‐time quantitative PCR. The expression of Bcl2 and Elovl5 was significantly up‐regulated (p < .05), while Cas8 expression (p < .05) and Bak/Bcl2 ratio were reduced (p < .02) in the cockerel testicles at 24 weeks of age. Although Bak mRNA abundance decreased by dietary LC, Bak/Bcl2 ratio was not affected by the treatments at 34 weeks of age. The expression of Cas3 was down‐regulated, while Fads2 was up‐regulated in the cockerel testicles by dietary LC at 34 weeks of age (p < .05). The results demonstrate the beneficial effects of LC supplementation in suppression of the Bak/Bcl2 ratio by altering Bak and Bcl2 mRNA abundance and, ultimately, prevention of apoptosis. Furthermore, LC increased the expression of Elovl5 and Fads2 genes which are involved in the metabolism of long chain fatty acids.

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Dietary Fish Oil and Vitamin E Reduce Cryoinjuries and Apoptosis in Vitrified Laying Hens' Ovarian Tissue

Cited by 6 publications

References 38 publications

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

Cryopreservation Competence of Chicken Oocytes as a Model of Endangered Wild Birds: Effects of Storage Time and Temperature on the Ovarian Follicle Survival

Dietary L‐carnitine affects the expression of genes involved in apoptosis and fatty acid metabolism in rooster testes

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