Dietary fiber extracted from pomelo fruitlets promotes intestinal functions, both in vitro and in vivo

Liu, Huifan; Zeng, Xinyue; JiaYong, Huang; Yuan, Xianhe; Wang, Qin; Ma, Lukai

doi:10.1016/j.carbpol.2020.117186

Cited by 58 publications

(45 citation statements)

References 39 publications

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“…Thereby, adding the dietary fiber into the chow increased the reproduction rates of well adapted bacterial taxa that can utilize dietary fiber, resulting in the variation in composition and structure of the gut bacteria in our pigs. Our results confirm this explanation, as the relative abundance of bacterial phyla (such as Spirochaetae and Fibrobacteres ) and bacterial genera (such as Turicibacter and unidentified Prevotellaceae ) which are able to degrade dietary fibers ( Mao et al, 2012 ; Le Sciellour et al, 2018 ; Calusinska et al, 2020 ; Liu et al, 2021 ) increased either in jejunum or cecum with the increase in dietary fiber ( Figures 1C,D ). Secondly, a high proportion of dietary fiber in chow negatively impacted the host digestibility of nutrients and energy ( Urriola and Stein, 2010 ), and was reported to induce an increase in bacteria (e.g., Bacteroides ), which can promote the utilization of more host polysaccharides and fat ( Sonnenburg et al, 2005 ; Wu et al, 2011 ), to balance energy expenditure.…”

Section: Discussionsupporting

confidence: 83%

Dietary Fiber Influences Bacterial Community Assembly Processes in the Gut Microbiota of Durco × Bamei Crossbred Pig

et al. 2021

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Several studies have shown that dietary fiber can significantly alter the composition and structure of the gut bacterial community in humans and mammals. However, few researches have been conducted on the dynamics of the bacterial community assembly across different graded levels of dietary fiber in different gut regions. To address this, 24 Durco × Bamei crossbred pigs were randomly assigned to four experimental chows comprising graded levels of dietary fiber. Results showed that the α-and β-diversity of the bacterial community was significantly different between the cecum and the jejunum. Adding fiber to the chow significantly increased the α-diversity of the bacterial community in the jejunum and cecum, while the β-diversity decreased. The complexity of the bacterial network increased with the increase of dietary fiber in jejunal content samples, while it decreased in cecal content samples. Furthermore, we found that stochastic processes governed the bacterial community assembly of low and medium dietary fiber groups of jejunal content samples, while deterministic processes dominated the high fiber group. In addition, deterministic processes dominated all cecal content samples. Taken together, the variation of gut community composition and structure in response to dietary fiber was distinct in different gut regions, and the dynamics of bacterial community assembly across the graded levels of dietary fiber in different gut regions was also distinct. These findings enhanced our knowledge on the bacterial community assembly processes in gut ecosystems of livestock.

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Section: Discussionsupporting

confidence: 83%

Dietary Fiber Influences Bacterial Community Assembly Processes in the Gut Microbiota of Durco × Bamei Crossbred Pig

et al. 2021

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“…It is believed that mitochondrial dysfunction of neurons can activate the mitochondrial unfolded protein response of intestine and promote the secretion of metabolic signals in response to misfolded protein stress [48]. We found in the present study that the signi cantly increased content of GABA in cortex and hippocampus of the diabetic mice were strongly related to the abundance of Bacteroides and Parabacteroides within gut lumen (Figure 3E and 7J), which is consistent with previous report [49,50]. On the other hand, TFA administration reduced expression of GABA in hippocampus, re-balanced composition of gut microbiota, promoted BBB integrity, reduced levels of in ammatory cytokines and AGEs, and preserved homeostasis of internal environment in the brain.…”

Section: Discussionsupporting

confidence: 93%

Intake of Flavonoids from Astragalus Membranaceus Ameliorated Brain Impairment in Diabetic Mice Via Modulating Brain-Gut Axis

Wang

et al. 2021

Preprint

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Background: Brain impairment is one of a major complication of diabetes. Dietary flavonoids have been recommended to prevent brain damage. Astragalus membranaceus is a herbal medicine commonly used to relieve the complications of diabetes. Flavonoids is one of the major ingredients of Astragalus membranaceus, but its function and mechanism on diabetic encepholopathy is still unknown.Methods: Type 2 diabetes mellitus (T2DM) model was induced by high fat diet and STZ in C57BL/6J mice, and BEnd.3 and HT22 cell lines were applied in the in vitro study. Quality of flavonoids was evaluated by LC-MS/MS. Differential expressed proteins in the hippocampus were evaluated by proteomics; influence of the flavonoids on composition of gut microbiota was analyzed by metagenomics. Mechanism of the flavonoids on diabetic encepholopathy was analyzed by Q-PCR, Western Blot, and multi-immunological methods et al. Results: We found that flavonoids from Astragalus membranaceus (TFA) significantly ameliorated brain damage by modulating gut-microbiota-brain axis: TFA oral administration decreased fasting blood glucose and food intake, repaired blood brain barrier, protected hippocampus synaptic function; improved hippocampus mitochondrial biosynthesis and energy metabolism; and enriched the intestinal microbiome in high fat diet/STZ-induced diabetic mice. In the in vitro study, we found TFA increased viability of HT22 cells and preserved gut barrier integrity in CaCO2 monocellular layer, and PGC1α/AMPK pathway participated in this process. Conclusion: Our findings demonstrated that flavonoids from Astragalus Membranaceus ameliorated brain impairment via gut-brain axis. Our present study provided an alternative solution on preventing and treating diabetic cognition impairment.

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“…During the period of inducing hyperglycemia on tested mice, the body weight of mice was found to decrease to 20.94 ± 2.92% and started continuously dropping to 47.23 ± 3.81% on day 21. A similar trend was observed in the study of Liu et al (2021). Glucophage-treated mice was found to have the return in the body weight after 21 days, from -18.85 ± 5.08% to 6.21 ± 5.33% (p < 0.05).…”

Section: Anti-hyperglycemic Effect Of Ppe In Vivosupporting

confidence: 86%

“…The body weight loss of Long Co Co albedo PPE-treated mice was attenuated to 4.56 ± 2.54%, whereas the Thanh Kieu albedo PPE-treated mice were found to have a weight gain of 1.21% on day 21. The increment of body weight of treated mice was observed after the therapy as the severity of hyperglycemia was attenuated (Liu et al, 2021).…”

Section: Anti-hyperglycemic Effect Of Ppe In Vivomentioning

confidence: 99%

“…Each 7-day interval, the body weight of model mice was recorded. After fasting for 18 h, blood samples from the orbital veins were collected and centrifuged at 1500×g for 20 min at 4 o C. The serum was stored at -20 °C, and blood glucose levels were analyzed using a Glucose Assay Kit (Merck KGaA, Darmstadt, Germany) (Liu et al, 2021).…”

Section: Anti-hyperglycemic Testmentioning

confidence: 99%

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Acute toxicity and anti-hyperglycemic activities of pomelo (Citrus grandis (L.) Osbeck) peel extracts in the Mekong Delta of Vietnam

et al. 2022

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Dietary fiber extracted from pomelo fruitlets promotes intestinal functions, both in vitro and in vivo

Cited by 58 publications

References 39 publications

Dietary Fiber Influences Bacterial Community Assembly Processes in the Gut Microbiota of Durco × Bamei Crossbred Pig

Dietary Fiber Influences Bacterial Community Assembly Processes in the Gut Microbiota of Durco × Bamei Crossbred Pig

Intake of Flavonoids from Astragalus Membranaceus Ameliorated Brain Impairment in Diabetic Mice Via Modulating Brain-Gut Axis

Acute toxicity and anti-hyperglycemic activities of pomelo (Citrus grandis (L.) Osbeck) peel extracts in the Mekong Delta of Vietnam

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