Dietary Fatty Acids Differentially Regulate Secretion of Adiponectin and Interleukin‐6 in Primary Canine Adipose Tissue Culture

Mazaki‐Tovi, Michal; Bolin, Steven R.; Schenck, Patricia A.

doi:10.1002/lipd.12021

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…This pattern of IL6 response to FA in feline adipose tissue may be viewed as consistent with the role of ARA in enhancing immune function [50]. These results in cats are different from our recent findings in canine adipose tissue SVC culture, where IL6 secretion was increased by PAM and decreased by EPA, while no effect of ARA was demonstrated [51], as well as results of studies in macrophages [52–54], adipocytes [55, 56], and endothelial cells [57].…”

Section: Discussionsupporting

confidence: 52%

Adipokines secretion in feline primary adipose tissue culture in response to dietary fatty acids

2019

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Background Obesity in cats has been associated with alterations in adipokines including: adiponectin, interleukin-6 (IL6), and tumor necrosis factor-α (TNFα). Omega-3 polyunsaturated fatty acids have multiple beneficial effects on obesity-associated disorders, and therefore may alleviate these alterations. This study aimed to determine the effects of body condition, fat depot, troglitazone, and different fatty acids on secretion of adiponectin, IL6 and TNFα from adipose tissue of healthy cats. Subcutaneous and visceral adipose tissue samples were collected from 18 healthy intact female cats, and body condition score (Range 3–7/9) was determined. Concentrations of adiponectin were measured in mature adipocytes cultures and concentrations of IL6 and TNFα were measured in stromovascular cells cultures following treatment with control medium, troglitazone at 10 μM, eicosapentaenoic acid, arachidonic acid, or palmitic acid, at 25, 50, or 100 μM. Results Stromovascular cells of visceral origin secreted higher concentrations of IL6 than corresponding cells of subcutaneous origin ( P = 0.003). Arachidonic acid treatment at 25, 50, and 100 μM increased IL6 secretion in subcutaneous ( P = 0.045, P = 0.002, and P < 0.001, respectively) and visceral ( P = 0.034, P = 0.001, and P < 0.001, respectively) stromovascular cells. Eicosapentaenoic acid treatment increased TNFα secretion in subcutaneous stromovascular cells at 25, 50, and 100 μM ( P = 0.002, P = 0.001, and P = 0.015, respectively) and in visceral stromovascular cells at 50 μM ( P < 0.001). No significant effect on medium adiponectin concentration was observed following troglitazone treatment ( P = 0.4) or fatty acids treatments at 25 ( P = 0.2), 50 ( P = 0.8), or 100 ( P = 0.7) μM. Body condition score did not have significant effects on medium concentrations of adiponectin ( P = 0.4), IL6 ( P = 0.1), or TNFα ( P = 0.8). Conclusions This study demonstrated higher basal secretion of IL6 from visceral compared to subcutaneous adipose tissue, a stimulatory effect of arachidonic acid on secretion of IL6 and a stimulatory effect of eicosapentaenoic acid on TNFα from feline adipose tissue.

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Section: Discussionsupporting

confidence: 52%

Adipokines secretion in feline primary adipose tissue culture in response to dietary fatty acids

2019

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Inflammatory crosstalk between saturated fatty acids and gut microbiota–white adipose tissue axis

Jamar

Pisani

2022

Eur J Nutr

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Effects of Omega-3 Polyunsaturated Fatty Acids on the Formation of Adipokines, Cytokines, and Oxylipins in Retroperitoneal Adi-Pose Tissue of Mice

Wenderoth,

Feldotto,

Hernandez

et al. 2024

IJMS

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Oxylipins and specialized pro-resolving lipid mediators (SPMs) derived from polyunsaturated fatty acids (PUFAs) are mediators that coordinate an active process of inflammation resolution. While these mediators have potential as circulating biomarkers for several disease states with inflammatory components, the source of plasma oxylipins/SPMs remains a matter of debate but may involve white adipose tissue (WAT). Here, we aimed to investigate to what extent high or low omega (n)-3 PUFA enrichment affects the production of cytokines and adipokines (RT-PCR), as well as oxylipins/SPMs (liquid chromatography–tandem mass spectrometry) in the WAT of mice during lipopolysaccharide (LPS)-induced systemic inflammation (intraperitoneal injection, 2.5 mg/kg, 24 h). For this purpose, n-3 PUFA genetically enriched mice (FAT-1), which endogenously synthesize n-3 PUFAs, were compared to wild-type mice (WT) and combined with n-3 PUFA-sufficient or deficient diets. LPS-induced systemic inflammation resulted in the decreased expression of most adipokines and interleukin-6 in WAT, whereas the n-3-sufficient diet increased them compared to the deficient diet. The n-6 PUFA arachidonic acid was decreased in WAT of FAT-1 mice, while n-3 derived PUFAs (eicosapentaenoic acid, docosahexaenoic acid) and their metabolites (oxylipins/SPMs) were increased in WAT by genetic and nutritional n-3 enrichment. Several oxylipins/SPMs were increased by LPS treatment in WAT compared to PBS-treated controls in genetically n-3 enriched FAT-1 mice. Overall, we show that WAT may significantly contribute to circulating oxylipin production. Moreover, n-3-sufficient or n-3-deficient diets alter adipokine production. The precise interplay between cytokines, adipokines, and oxylipins remains to be further investigated.

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Dietary Fatty Acids Differentially Regulate Secretion of Adiponectin and Interleukin‐6 in Primary Canine Adipose Tissue Culture

Cited by 3 publications

References 69 publications

Adipokines secretion in feline primary adipose tissue culture in response to dietary fatty acids

Adipokines secretion in feline primary adipose tissue culture in response to dietary fatty acids

Inflammatory crosstalk between saturated fatty acids and gut microbiota–white adipose tissue axis

Effects of Omega-3 Polyunsaturated Fatty Acids on the Formation of Adipokines, Cytokines, and Oxylipins in Retroperitoneal Adi-Pose Tissue of Mice

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