Dietary fatty acids are possible key determinants of cellular retinol-binding protein II gene expression

Takase, Sachiko; Tanaka, Kimiko; Suruga, Kazuhito; Kitagawa, Masaaki; Igarashi, Miki; Goda, Toshinao

doi:10.1152/ajpgi.1998.274.4.g626

Cited by 16 publications

(16 citation statements)

References 28 publications

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“…It is speculated that retinol/␤-carotein digestion/absorption related proteins including CRBPII may address their digestion/absorption by synchronously expressing in the villus. The present study extends our previous finding which showed that dietary fat elicited or enhanced the levels of CRBPII mRNA in the rat jejunum (8,9). The high-fat diet induced an increase in CRBPII mRNA as early as 12 h after consumption (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…The high-fat diet induced an increase in CRBPII mRNA as early as 12 h after consumption (Fig. 2), while it is already confirmed that feeding a low-fat diet does not induce CRBPII gene expression in adult (8) or weaning rats (17). The cryostat slicing technique enables us to determine the precise locus of villus cells where the rapid mRNA accumulation occurred.…”

Section: Discussionmentioning

confidence: 53%

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Distribution and Dietary Induction of Cellular Retinol-Binding Protein Type II along the Villus-Crypt Axis of the Rat Jejunum

Ogura

Yasutake

Mochizuki

et al. 2008

J Nutr Sci Vitaminol

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Summary Cellular retinol-binding protein type II (CRBPII) is exclusively expressed in the small intestinal absorptive cells. We previously reported that dietary fat induces CRBPII expression within 12 h of fat intake. To examine at which locus of the villus-crypt axis this response to dietary fat occurs, 6-wk-old rats were fed a low-fat diet (7% energy) for 7 d, and then given free access to a high-fat diet (70% energy) for the subsequent 12, 24 or 48 h. Cryostat sectioning of jejunal segments followed by RNA blot hybridization of the transcripts revealed that CRBPII mRNA was expressed maximally in the lower villus, and the immunoreactive protein of CRBPII was expressed maximally in the mid-villus. Feeding the highfat diet caused a pronounced increase in CRBPII mRNA level from the lower-to middle-villus within 12 h. These results suggest that the CRBPII gene is maximally expressed in the lower villus, and that dietary fat causes an enhancement of CRBPII gene expression in the villus cells. Key Words CRBPII, high-fat diet, rats, villus, crypt Cellular retinol-binding protein type II (CRBPII) is exclusively expressed in the small intestinal villus cells and plays a pivotal role in intestinal absorption and metabolism of retinol and ␤ -carotene. CRBPII not only directs retinal reductase to convert retinal to retinol in enterocytes, but also directs lecithin:retinol acyltransferase (L-RAT) to convert retinol to retinyl esters, which leads to incorporation of vitamin A into chylomicron ( 1 ). Like many other genes involved in intestinal nutrient absorption ( 2 -5 ), the CRBPII gene is thought to be expressed at certain stages of the differentiation or maturation of intestinal absorptive cells, which are derived from intestinal stem cells in the crypt and continuously differentiate into the cells destined for nutrient absorption. We have previously demonstrated that CRBPII protein and the enzyme activities involved in vitamin A absorption, e.g. ␤ -carotene cleavage enzyme, retinal reductase and L-RAT, are coordinately expressed along the villus-crypt axis in the small intestine ( 6 , 7 ). However, the precise distribution of CRBPII mRNA along the villus-crypt axis was unclear. We previously showed that the distribution of the gene transcripts for lactasephlorizin hydrolase (LPH) is more apical and broader than that of sucrase-isomaltase (SI) along the crypt-villus axis, and that dietary sucrose specifically enhances SI mRNA level in the lower villus and LPH mRNA level in the middle and upper villi ( 5 ). These results suggest that nutrients enhance intestinal gene expression in specific phase(s) of differentiation in absorptive cells along the villus-crypt column. Our previous studies showed that dietary fat is a major regulator of CRBPII gene expression ( 8 , 9 ), and that the fat-induced increase in CRBPII mRNA occurs within 12 h of fat exposure, via one of the nuclear receptors, PPAR ␣ ( 10 , 11 ). It is most likely that expression of the CRBPII gene is regulated by dietary fat at a certain stage of maturation in t...

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 53%

Distribution and Dietary Induction of Cellular Retinol-Binding Protein Type II along the Villus-Crypt Axis of the Rat Jejunum

Ogura

Yasutake

Mochizuki

et al. 2008

J Nutr Sci Vitaminol

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“…Studies in our own and other laboratories have demonstrated that expression of the genes involved in ␤-oxidation and absorption of fatty acids and retinol in the small intestine are regulated by PPAR (19,21,30,36,37). Moreover, our previous studies suggest that dietary fat-induced alterations of small intestinal L-FABP and CRBPII gene expression may be regulated by the expression change of PPAR␣ (20,25,31,33). Recently, we have shown that the expression of small intestinal PPAR-dependent genes are up-regulated by clofibrate which is a known PPAR␣ activator (22).…”

Section: )mentioning

confidence: 84%

“…Our own and other laboratories have demonstrated that liver-type (L-FABP) and intestine-type (I-FABP) fatty acid-binding proteins, which are proteins mediating fatty acid absorption from lumen to enterocyte, are induced by dietary fat in adult rats (19,20,22,30). Additionally, previous studies have demonstrated that the gene expression of cellular retinol binding protein (CRBPII), which is a protein allowing absorption of retinol from lumen to enterocyte, is up-regulated by dietary fat (21,22,25,(31)(32)(33). Recent studies have shown that these genes, including L-FABP and CRBPII, possess a direct repeat with one space (DR-1) of AGGTCA-like motifs in their promoter regions (11, 34, …”

Section: Discussionmentioning

confidence: 99%

Possible Role of Fatty Acids in Milk as the Regulator of the Expression of Cytosolic Binding Proteins for Fatty Acids and Vitamin A through PPAR.ALPHA. in Developing Rats

Mochizuki

Kawai

et al. 2007

J Nutr Sci Vitaminol

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Summary Fatty acids in milk are thought to play an important role in intestinal maturation and gene expression in the postnatal small intestine. In this study, we determined the jejunal mRNA levels, in rats, of peroxisome proliferator-activated receptor ␣ (PPAR ␣ ) and PPAR ␦ which are nuclear receptors for fatty acids. We also measured expression of their target genes during the postnatal period, namely liver type fatty acid-binding protein (L-FABP) and cellular retinol-binding protein, type II (CRBPII). The mRNA levels of PPAR ␣ , L-FABP and CRBPII, but not PPAR ␦ , gradually increased during the suckling period and then sharply declined to a low level at the end of the weaning period. Rat pups at 17 d of age, weaned to a high-fat diet, showed significantly greater mRNA levels of PPAR ␣ , L-FABP and CRBPII than those weaned to a low-fat diet. Oral administration of PPAR ␣ ligand, WY14,643 during four consecutive days of the weanling period caused a parallel increase in the mRNA levels of PPAR ␣ , L-FABP and CRBPII genes. Furthermore, caprylic acid and oleic acid, which are major components of fatty acids in milk, induced jejunal PPAR ␣ , L-FABP and CRBPII gene expression. Our results suggest that fatty acids in milk may play a pivotal role in maintaining an enhanced level of expression of L-FABP and CRBPII genes in the small intestine, presumably by acting as inducers of PPAR ␣ gene expression.

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“…shows that genes associated with folate and clinical conditions (115). It has studied that the administration of 9-cis retinoic acid to such animals brought about no accumulation of the CRBPII mRNA (116). So, in spite of vitamin A deficiency, oral administration of corn oil, but not 9-cis retinoic acid, caused an increase in jejunal CRBPII mRNA level.…”

Section: The Interactions Between Most Fortificated and Dietary Bioactimentioning

confidence: 99%

Most fortificated nutraceutical vitamins: standpoint from biochemistry, functionality, utilization strategies and medical genomics

Tokuşoğlu

Müezzinoğlu

2015

Natural Science and Discovery

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Nutraceutical vitamins are micronutrients of foods and beverages that play an essential role in food science and technology. Most fortificated vitamin A, E, D and folic acid as antioxidative defence system components and that of with gene regulation functions have been considered. Up-to-date scientific overview of the biochemistry, functionality, utilization strategies and human genomics of above-mentioned most fortificated nutraceutical vitamins were emphasized

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Dietary fatty acids are possible key determinants of cellular retinol-binding protein II gene expression

Cited by 16 publications

References 28 publications

Distribution and Dietary Induction of Cellular Retinol-Binding Protein Type II along the Villus-Crypt Axis of the Rat Jejunum

Distribution and Dietary Induction of Cellular Retinol-Binding Protein Type II along the Villus-Crypt Axis of the Rat Jejunum

Possible Role of Fatty Acids in Milk as the Regulator of the Expression of Cytosolic Binding Proteins for Fatty Acids and Vitamin A through PPAR.ALPHA. in Developing Rats

Most fortificated nutraceutical vitamins: standpoint from biochemistry, functionality, utilization strategies and medical genomics

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