Dietary factors affecting uterine weights of immature CD-1 mice used in uterotrophic bioassays

Tools to evaluate estrogenic potency of dietary phytoestrogens:A consensus paper from the EU Thematic Network “Phytohealth” (QLKI-2002-2453)

“…These diets are composed of refined, invariant, and restricted set of ingredients, which offer less variable and more easily controlled experimental conditions. Additionally, the use of purified or synthetic diets instead of natural ingredient chows, gives more precise control over the metabolizable energy, dietary composition of nutrient, and provides better repeatable experimental conditions (Ritskes-Hoitinga, 2001;Thigpen et al, 2002;Odum et al, 2004). However, it is still an open question if the experimental diet should be a standard for the chosen species or should mimic human Western diet with regard to fat, fibre, and calcium content.…”

Apoptosis of human primary gastric carcinoma cells induced by genistein

“…5 /well in a 96-well plate after incubation for 24 h were treated with different concentrations of genistein (5,10,20, 40 µmol/ L) for 24, 48 and 72 h respectively. A 10 µL of 5 g/L of MTT was added to the medium triplicate at each dose and incubated for 4 h at 37 .…”

“…It has estrogenic properties in receptor binding assays [1,2] , cell culture [3,4] , and uterine weight assays [5][6][7] . Genistein inhibits topoisomerase II [8] , platelet-activating factor-and epidermal growth factor-induced expression of c-fos [9] , diacylglycerol synthesis [10] , and tyrosine kinases [11] .…”

Anticancer activity of genistein on implanted tumor of human SG7901 cells in nude mice

“…Genistein is a planar molecule with an aromatic A-ring, has a second oxygen atom from that in the A ring, and has a molecular mass similar to those of the steroidal estrogens. It has estrogenic properties in receptor binding assays [15,16] , cell culture [17,18] , and uterine weight assays [19][20][21] . Genistein inhibits topoisomerase Ⅱ [33] , platelet-activating factor-and epidermal growth factorinduced expression of c-fos [34] , diacylglycerol synthesis [35] , and tyrosine kinases [36] .…”

“…Genistein has estrogenic properties in receptor binding assays [15,16] , cell culture [17,18] , and uterine weight assays [19][20][21] . Genistein inhibits microsomal lipid peroxidation [22] and angiogenesis [23] .…”